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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(1): 57-60


Mechanism of antiseizure effect of isoflurane in the immature rat hippocampus

E.V. Isaeva

    O.O.Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz55.01.057

Abstract

The volatile anesthetic isoflurane is often used in children in the management of refractory status epilepticus. However the mechanism of anticonvulsant action of isoflurane during early brain development is not clear. In this study we explore the role of excitatory and inhibitory conductances in antiseizure effect of isoflurane using combination of whole-cell patch-clamp and extracellular field potential recording techniques on two models of epilepsy in a hippocampal slice preparation from immature rat. Our data demonstrated that decreasing of excitatory sy-naptic transmission does not account for antiseizure effect of this volatile anesthetic agent. Isoflurane decreases the synchro­nization of neuronal activity mainly through the enhancing of GABAergic inhibition by influencing both phasic and tonic chloride conductances.

Keywords: isoflurane

References

  1. Antkowiak B. Different actions of general anesthetics on the firing patterns of neocortical neurons mediated by the GABA(A) receptor . Anesthesiol. 1999. 91. P. 500-511. CrossRef PubMed
  2. Banks M.I., Pearce R.A. Dual actions of volatile anesthetics on GABA(A) IPSCs: dissociation of block­ing and prolonging effects . Anesthesiol. 1999. 90. P.120-134. CrossRef PubMed
  3. Hilz M.J., Bauer J., Claus D. et al. Isoflurane anaesthesia in the treatment of convulsive status epilepticus. Case report . J. Neurol. 1992. 239. P.135-137. CrossRef PubMed
  4. Isaeva E. V. Effects of isoflurane on hippocampal sei­zures at immature rats in vivo. Fiziol.J. 2008. 5. P.
  5. Isaeva E., Isaev D., Holmes G. L. Anesthetic and postanes-thetic effects of isoflurane on neuronal activity in the rat hippocampus. Neurophysiology. 2007. 39. P.325-326. CrossRef
  6. Kofke W.A., Young R.S.K., Davis P. et al. Isoflurane for refractory status epilepticus: A clinical series . Anesthesiol. 1989. 71. P.653-659. CrossRef PubMed
  7. Maclver M.B., Mikulec A.A., Amagasu S.M., Monroe F.A. Volatile anesthetics depress glutamate transmis­sion via presynaptic actions . Anesthesiol. 1996. 85. P.823-834. CrossRef PubMed
  8. Prenosil G.A., Schneider Gasser E.M., Rudolph U. et al. Specific subtypes of GABAA receptors mediate phasic and tonic forms of inhibition in hippocampal pyramidal neurons . J. Neurophysiol. 2006. 96. P.846-857. CrossRef PubMed
  9. Sahin M., Menache C.C., Holmes G.L., Riviello J.J. Outcome of severe refractory status epilepticus in children . Epilepsia. 2001. 42. P.1461-1467. CrossRef PubMed
  10. Sakaki T., Abe K., Hoshida T. et al. Isoflurane in the manage­ment of status epilepticus after surgery for lesion around the motor area . Acta Neurchir. 1992. 116. P.38-43. CrossRef PubMed
  11. Stell B.M., Mody I. Receptors with different affinities mediate phasic and tonic GABA(A) conductances in hip-pocampal neurons . J. Neurosci. 2002. 15. P.1-5.

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