Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(1): 49-56


A.I. Bondarenko

    O.O. Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv, Ukraine


The influence of pinacidil, an activator of ATP-sensitive K+ channels, on the membrane potential of endothelial cells from intact rat aorta and cultured endothelial cells was investigated. Pinacidil evoked a slowly developing sustained hyperpolariza-tion of endothelial cells from isolated artery with the amplitude of 15±4 mV from the resting membrane potential of –4Ш мВ. In contrast, in cultured endothelial cells pinacidil was without response. Diazoxide, another activator of ATP-sensitive K+ channels, in half of the cultured cells tested, evoked a slowly developing sustained hyperpolarization with the amplitude of 3 mV. The rest of the cells studied did not respond by membrane potential changes to diazoxide. It was suggested that high sen­sitivity of the membrane potential of in situ endothelial cells to potassium channels openers may represent a potent signaling mechanism influencing endothelial cell function upon stimula­tion of vascular KATP channels.

Keywords: pinacidil


  1. Bondarenko AI, Sagach VF Modulation of the membrane potential of cells of the intact endothelium of the guinea pig aorta . Neurophysiology. 1996. 28, N 6. S.260-266. CrossRef
  2. Bondarenko OI, Prisyazhna OD, Sagach VF Electrical reactions of intact rat aortic endothelium in experimental diabetes . Fiziol Zh. 2004. 50, N 6. C 3-8.
  3. Bondarenko OI, Sagach VF Electrical reactions of the aortic endothelium of rats with spontaneous hypertension . Fiziol Zh. 2002. 48, N 4. P.75-79.
  4. Nagibin B.C., Dosenko B.C., Brewer SM Fluorinated diazoxide analogue prevents apoptosis of neonatal cardiomyocytes during anoxia-reoxygenation. Fiziol Zh. 2004. 50, N 3. P.3-8.
  5. Brewer SM., Strutynsky RB, Yagupolsky LM, Moybenko OO Investigation of the mechanisms of action of new fluorine-containing analogues of diaoxide on vascular tone . Fiziol Zh. 2004. 50, N 2. P.27-33.
  6. Chekman IS, Tarasova KV, Shevchuk VG Physiological properties and prospects of correction of the function of adeno-zintriphosphate potassium channels . Fiziol Zh. 2008. 54, N 1. S94-107.
  7. Yarotsky VV, Tkachenko MN Sagach VF Electrical reactions of rat aortic endothelium under the action of acetylcholine and ATP under aging conditions . Bull. experiment. biology and medicine. 2003. 135, N 3. C257-260. CrossRef PubMed
  8. Aguilar-Bryan L., Clement J.P., Gonzalez G. et al. To­ward understanding the assembly and structure of KATP channels . Physiol Rev. 1998. 78. P.227-245 CrossRef PubMed
  9. Bondarenko A. Sodium-calcium exchanger contributes to membrane hyperpolarization of intact endothelial cells from rat aorta during acetylcholine stimulation . Brit. J.Pharmacol. 2004. 143. P.9-18. CrossRef PubMed PubMedCentral
  10. Brayden J.E. Functional roles of KATP channels in vascular smooth muscle . Clin. Exp. Pharmacol. Physiol. 2002. 29. P.312-316. CrossRef PubMed
  11. Bychkov R., Gollasch M., Ried C. et al. Effects of pinacidil on K+ channels in human coronary artery vas­cular smooth muscle cells . Amer. J. Physiol. 1997. 273. C161-C171. CrossRef PubMed
  12. DrieuLaRochelle C, Richard V., Dubois-Rande J.L. et al. Potassium channel openers dilate large epicardial coronary arteries in conscious dogs by an indirect, en-dothelium-dependent mechanism . J. Pharmacol. Exp. Therap . 1992. 263. P.1091-1096.
  13. Engler M.B., Engler M.M. Docosahexaenoic acid-in­duced vasorelaxation in hypertensive rats: mechanisms of action . Biol. Res. Nurs. 2000. 2. P.85-95. CrossRef PubMed
  14. Feleder E.C, Adler-Graschinsky E. Endothelium-mediated and N omega-nitro-L-arginine methyl ester-sensitive responses to cromakalim and diazoxide in the rat mesenteric bed. Eur. J. Pharmacol. 1997. 319. P.229-238. CrossRef
  15. Gasser R., Klein W., Kickenweiz E. Vasodilative response to hypoxia and simulated ischemia is mediated by ATP-sensitive K+ channels in guinea pig thoracic aorta . Angiology. 1993. 44. P.228-243. CrossRef PubMed
  16. Ghosh M., Hanna ST., Wang R., McNeill J.R. Altered vascular reactivity and KATP channel currents in vas­cular smooth muscle cells from deoxycorticosterone acetate (DOCA)-salt hypertensive rats . J. Cardiovas­cular. Pharmacol. 2004. 44. P.525-531. CrossRef PubMed
  17. Janigro D., Nguyen T.S., Meno J. et al. Endothelium-dependent regulation of cerebrovascular tone by ex­tracellular and intracellular ATP . Amer. J. Physiol. 1997. 273. H878-H885. CrossRef PubMed
  18. Janigro D., West G.A., Gordon E.L., Winn H.R. ATP-sensitive K+ channels in rat aorta and brain microvascu­lar endothelial cells . Ibid. 1993. 265. C812-C821. CrossRef PubMed
  19. Kalsner S. Hypoxic relaxation in functionally intact cattle coronary artery segments involves K+ ATP channels . J. Pharmacol. Exp. Therap . 1995. 275. C.1219-1226.
  20. Katnik C, Adams D.J. Characterization of ATP-sensi­tive potassium channels in freshly dissociated rabbit aortic endothelial cells . Amer. J. Physiol. 1997. 272. H2507-H2511. CrossRef PubMed
  21. Lahaye P., Fouassier L., Tazi K.A. et al. Endothelium-dependent blunted membrane potential responses to ATP-sensitive K+ channel modulators in aortae from rats withcirrhosis . J. Hepatol. 1999. 30. P.107-114. CrossRef
  22. Langheinrich U., Schnitzler M., Daut J. Ca2+-transients induced by K+ channel openers in isolated coronary capillaries . Pflug. Arch. 1998. 435. P.435-438. CrossRef PubMed
  23. Marchenko S.M., Sage S.O. Electrical properties of resting and acetylcholine-stimulated endothelium in in­tact rat aorta . J.Physiol. 1993. 462. P.735-751. CrossRef PubMed PubMedCentral
  24. Mehrke G., Pohl U., Daut J. Effects of vasoactive ago­nists on the membrane potential of cultured bovine aortic and guinea-pig coronary endothelium . J. Physiol. 1991. 439. P. 277-299. CrossRef PubMed PubMedCentral
  25. Minamino T., Hori M. Protecting endothelial function: A novel therapeutic target of ATP-sensitive potassium channel openers . Cardiovascular. Res. 2007. 73. P.448-449. CrossRef PubMed
  26. Murai T., Muraki K., Imaizumi Y, Watanabe M. Levcro-makalim causes indirect endothelial hyperpolarization via a myo-endothelial pathway . Br. J. Pharmacol. 1999. 128. P.1491-1496. CrossRef PubMed PubMedCentral
  27. Olanrewaju H.A., Gafurov B.S., Lieberman E.M. Involvement of K+ channels in adenosine A2A and A2B receptor-mediated hyperpolarization of porcine coronary artery endothelial cells . J. Cardiovascular. Pharmacol. 2002. 40. P.43-49. CrossRef PubMed
  28. Quast U., Guillon J.M., Cavero I. Cellular pharmacol­ogy of potassium channel openers in vascular smooth muscle . Cardiovascular. Res. 1994. 28. P.805-810. CrossRef PubMed
  29. Quayle J.M., Nelson M.T., Standen N.B. ATP-sensitive and inwardly rectifying potassium channels in smooth muscle . Physiol. Rev. 1997. 77. P.1165-1232. CrossRef PubMed
  30. Sampson L.J., Hayabuchi Y., Standen N.B., Dart C. Caveolae localize protein kinase A signaling to arterial ATP-sensitive potassium channels . Circulat. Res. 2004. 95. P.1012-1018. CrossRef PubMed
  31. Simard J.M., Tsymbalyuk O., Ivanov A. et al. Endothe­lial sulfonylurea receptor 1-regulated NC Ca-ATP channels mediate progressive hemorrhagic necrosis fol­lowing spinal cord injury . J. Clin. Invest. 2007. 117. P.2105-2113. CrossRef PubMed PubMedCentral
  32. Taguchi H., Faraci F.M., Kitazono T., Heistad D.D. Relaxation of the carotid artery to hypoxia is impaired in Watanabe heritable hyperlipidemic rabbits . Arterioscler. Thromb. Vasc. Biol. 1995. 15. P.1641-1645. CrossRef PubMed
  33. Taguchi H., Faraci F.M., Kitazono T., Heistad D.D. Rela­xation of the aorta during hypoxia is impaired in chronically hypertensive rats . Hypertension. 1995. 25. P.735-738. CrossRef PubMed
  34. Usachev Y.M., Marchenko S.M., Sage S.O. Cytosolic calcium concentration in resting and stimulated endot­helium of excised intact rat aorta . J. Physiol. 1995. 489 (Pt 2). P.309-317. CrossRef PubMed PubMedCentral
  35. White R., Hiley C.R. Hyperpolarisation of rat mesenteric endothelial cells by ATP-sensitive K(+) channel open­ers . Eur. J. Pharmacol. 2000. 397. P.279-290. CrossRef
  36. White R., Hiley C.R. Endothelium and cannabinoid receptor involvement in levcromakalim vasorelaxation . Eur. J. Pharmacol. 1997. 339. P.157-160. CrossRef
  37. Yang B.C., Mehta J.L. Critical role of endothelium in sustained arterial contraction during prolonged hypoxia . Amer. J. Physiol. 1995. 268. H1015-H1020. CrossRef PubMed
  38. Zharikov S.I., Herrera H., Block E.R. Role of membrane potential in hypoxic inhibition of L-arginine uptake by lung endothelial cells . Ibid. 1997. 272. L78-L84. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.