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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(1): 43-48


A.V. Kubyshkin, I.I. Fomochkina

    The Crimea State Medical University, Simferopol, Ukraine


We studied the influence of a way of introduction proteinase inhibitors on efficiency of suppression of proteolysis activation during pneumonia. Comparative study of efficiency of proteases inhibition in experimental pneumonia has shown higher efficacy of local introduction of drugs. Intravenous and intraperitoneal introduction of proteinase inhibitors exhibited inhibitory effect of a smaller degree on local and systemic proteases activation, did not decrease an acute phase of response of a-1-protease inhibitor in comparison with endotracheal instillation of Contrycal and Ingiprol. The study has established that endotracheal intro­duction of proteinase inhibitors is the most effective for correction of the proteinase-inhibitor balance. It also helps to promote the activity proteinase-inhibitor, suppresses elastolytic activity, de­creases cellular infiltration, reduces the concentration of pro­teins in bronco-alveolar lavage fluid that is connected with ad­dress delivery of drugs to the target organ creating a maximal concentration of drugs in affected area.

Keywords: proteinase inhibitors


  1. Zakharievskaya MA, Anichkov NN On changes in lung tissue with the introduction of a foreign body into the bronchi . Bull. experiment. biology and medicine. 1952. N 6. P. 62-67.
  2. Krinskaya AV, Paskhina TS Quantitative determination of kallikrein and kallikreinogen in human blood serum (plasma) . Sovr. methods of biochemistry. M .: Medicine, 1977. P. 163-170.
  3. Kubyshkin AV, Donetsk IA, Balabushevich NG, Makarevich IA Comparative study of a new pancreatic proteinase inhibitor in the initiation of inflammation in the lungs . Chem. Pharm. magazine. 1993. N 3. p. 17-19. CrossRef
  4. Nartykova VF, Pashina TS Unified method for determining the activity of a1-antitrypsin and a-macroglobulin in human serum (plasma) . Vopr. honey. chemistry. 1979. N 4. P. 494-499.
  5. Ogloblina OG, Platonova LV, Myasnikova LV et al. The activity of granulocyte proteinases and the level of acid-stable protease inhibitors in the broncho-alveolar secretion of children with bronchopathy of various etiologies . Ibid. 1980. N 3. p. 387-392.
  6. Semenkova GG, Silvestrov VP, Provotorov VM Immediate and long-term results of treatment of patients with non-specific lung diseases through local application of contraceptives and antibiotics . Therapy. archive. 1992. 64, N 3. p. 66-71.
  7. Tinney D. Introduction to the theory of experimental planning. M .: Nauka, 1970. p. 33.
  8. Shulpekova Yu.O. Aprotinin an important component of the therapy of critical conditions . Rus. honey. magazine. 2000. 8, N 7. p. 296-299.
  9. Douvas S., Kolokotronis A., Stefanopoulos P. Anti-Inflammatory and antimicrobial roles of secretory leukocyte protease inhibitor . Inf. and Immunol. 2005. 73, N 3. P. 1271-1274. CrossRef PubMed PubMedCentral
  10. Griese M., Latzin P., Kappler M. et al. Alpha1-Antit-rypsin inhalation reduces airway inflammation in cystic fibrosis patients . Eur. Respir. J. 2007. 29, N 2. P. 240-250. CrossRef PubMed
  11. Hook V. Y, Hwang S.R. Novel secretory vesicle serpins, endopin 1 and endopin 2: endogenous protease inhibi­tors with distinct target protease specificities . Biol. Chem. 2002. 383, N 7-8. P. 1067-1074. CrossRef PubMed
  12. Lowry O., Rosenbrough U., Farr A., Roundal R. Pro­tein measurement with the Folin phenol reagent . J. Biol. Chem. 1951. 193, N 1. P. 265-275. CrossRef
  13. Okumura Y, Ogawa K., Uchiya K. Characterization and primary structure of elastase inhibitor, AFLEI, from Aspergillus flavus . Nippon. Ishinkin. Gakkai. Zasshi. 2007. 48, N 1. P. 13-18. CrossRef PubMed
  14. Ovcharenko A.V., Zhirnov O.P. Aprotinin aerosol treat­ment of influenza and paramyxovirus bronchopneumo­nia of mice . Antiviral. Res. 1994. 23, N 2. P. 107-118. CrossRef
  15. Vender R.L. Therapeutic potential of neutrophil-elastase inhibition in pulmonary disease . J. Invest. Med. 1996. 44. P. 531-539.
  16. Wei S., Chen Y, Chung L. et al. Protein engineering of the tissue inhibitor of metalloproteinase 1 (TIMP-1) in­hibitory domain. In search of selective matrix metalloproteinase inhibitors . J. Biol. Chem. 2003. 278, N 11. P. 9831-9834. CrossRef PubMed
  17. Zheng C.J., Han L.Y, Yap C.W. et al. Therapeutic tar­gets: progress of their exploration and investigation of their characteristics . Pharmacol. Rev. 2006. 58, N 2. P. 259-279. CrossRef PubMed

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