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Functional activity of hippocampalneurons after short-term oxygen-glucose deprivation in vitro
I.V.Lushnikova.
A.A.Bogomoletz Institute of Physiology NASU,Kiev, Ukraine
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Abstract
The deficiency of oxygen and glucose induces neuronal cell
damage and death through multiple cellular and molecular
mechanisms. The degree of the damage is thought to depend
on the intensity and duration of pathological event. In vivo and
in vitro experimental investigations have shown that short-term
oxygen-glucose deprivation (OGD) results in the delayed cell
destruction. It allows studying of the neurodegenerative mecha-
nisms induced by OGD for possible corrections. The aim of the
present study was to evaluate the morpho-functional changes
in cultured hippocampal neurons after short-term OGD followed
by reoxygenation during 1, 4 or 24 h. It is revealed that 20 min
OGD has no influence on the cell viability, but metabolic activ-
ity, synaptic quantity and activity are decreased at 1 h at 4 h
after OGD. These functional characteristics are normalized at
24h. The results expand a concept about different aspects of
hippocampal neurons injury under deficiency of oxygen and glu-
cose. A suggested experimental model could be used for testing
different neuroprotective tools.
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