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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(6): 58-65


Functional activity of hippocampalneurons after short-term oxygen-glucose deprivation in vitro

I.V.Lushnikova.

    A.A.Bogomoletz Institute of Physiology NASU,Kiev, Ukraine


Abstract

The deficiency of oxygen and glucose induces neuronal cell damage and death through multiple cellular and molecular mechanisms. The degree of the damage is thought to depend on the intensity and duration of pathological event. In vivo and in vitro experimental investigations have shown that short-term oxygen-glucose deprivation (OGD) results in the delayed cell destruction. It allows studying of the neurodegenerative mecha- nisms induced by OGD for possible corrections. The aim of the present study was to evaluate the morpho-functional changes in cultured hippocampal neurons after short-term OGD followed by reoxygenation during 1, 4 or 24 h. It is revealed that 20 min OGD has no influence on the cell viability, but metabolic activ- ity, synaptic quantity and activity are decreased at 1 h at 4 h after OGD. These functional characteristics are normalized at 24h. The results expand a concept about different aspects of hippocampal neurons injury under deficiency of oxygen and glu- cose. A suggested experimental model could be used for testing different neuroprotective tools.

References

  1. Гусев Е.И., Скворцова В.И. Ишемия головногомозга. – М.: Медицина, 2001. – 328 с.
  2. Винничук С.М., Черенько Т.М. Ишемическийинсульт: эволюция взглядов на стратегию лечения –К.: ООО Комполис, 2003. – 120 с.
  3. Bolay H., Gursoy-Ozdemir Y., Sara Y. et al. Persistentdefect in transmitter release and synapsin phosphoryla-tion in cerebral cortex after transient moderate ischemicinjury // Stroke. – 2002. – 33, № 5. – Р.1369–1375.
  4. Castillo P.E., Schoch S., Schmitz F. et al. RIM1alpha isrequired for presynaptic long-term potentiation // Na-ture. – 2002. – 415, № 6869. – Р.327–330.
  5. Genoud S., Pralong W., Riederer B.M. et al. Activity-dependent phosphorylation of SNAP-25 in hippocampalorganotypic cultures // J.Neurochem. – 1999. – 72,№ 4. – Р.1699–1706.
  6. Harata N., Ryan T., Smith S.et al. Visualizing recyclingsynaptic vesicles in hippocampal neurons by FM 1-43photoconversion // Proc. Natl. Acad. Sci. USA. – 2001. –98, № 22. – P.12748–12753.
  7. Janz R., Sьdhof T.C., Hammer R.E. et al. Essential rolesin synaptic plasticity for synaptogyrin I and synapto-physin I // Neuron. – 1999. – 24, № 3. – Р.687–700.
  8. Jourdain P., Nikonenko I., Alberi S. et al. Remodelingof hippocampal synaptic networks by a brief anoxia-hypoglycemia // J.Neurosci. – 2002. – 22, № 8. –Р.3108–3116.
  9. 9. Jung Y.J., Park S.J., Park J.S. et al. Glucose/oxygendeprivation induces the alteration of synapsin I andphosphosynapsin // Brain Res. – 2004. – 996, № 1. Р.47–54.
  10. 10. Kovalenko T., Osadchenko I., Nikonenko A. et al. Is-chemia-induced modifications in hippocampal CA1 stra-tum radiatum excitatory synapses // Hippocampus. –2006. – 16, № 10. – Р.814–825.
  11. Lipton P. Ischemic cell death in brain neurons // Physiol.Rev. – 1999. – 79, № 4. – P.1431–1568.
  12. Lukyanetz E.A., Shkryl V.M., Kravchuk O.V., KostyukP.G. Effect of hypoxia on calcium channels depends onextracellular calcium in CA1 hippocampal neurons //Brain Res. – 2003. – 980, № 1. – Р. 128–134.
  13. Lushnikova I.V., Voronin K., Malyarevskyy P.Y. et al.Morphological and functional changes in rat hippocam-pal slice cultures after short-term oxygen-glucose depri-vation // J.Cell Mol.Med. – 2004. – 8, № 2. – Р.241–248.
  14. Maar T., Rшnn L., Bock E. et al. Characterization ofmicrowell cultures of dissociated brain tissue for stud-ies of cell-cell interaction // J. Neurosci. Res. – 1997. –47, № 2. – P.163–172.
  15. Misonou H., Mohapatra D.P., Menegola M., TrimmerJ.S. Calcium- and metabolic state-dependent modula-tion of the voltage-dependent Kv2.1 channel regulatesneuronal excitability in response to ischemia // J. Neu-rosci. – 2005. – 25, № 48. –P.11184–11193.
  16. Nishimura H., Matsuyama T., Obata K. et al. Changesin mint1, a novel synaptic protein, after transient globalischemia in mouse hippocampus // J.Cereb.BloodFlow.Metab. – 2000. – 20, № 10. – Р.1437–1445.
  17. Ring G., Mezza R., Schwob J. Immunohistochemicalidentification of discrete subsets of rat olfactory neuronsand the glomeruli that they innervate // J. Comp. Neurol. –1997. – 388, № 3. – Р.415–434.
  18. Pozzo-Miller L.D., Gottschalk W., Zhang L. et al.Impairments in high-frequency transmission, synapticvesicle docking, and synaptic protein distribution in thehippocampus of BDNF knockout mice // J.Neurosci. –1999. – 19, № 12. – Р.4972–4983.
  19. 19. Priller C., Bauer T., Mitteregger G., Krebs B.,Kretzschmar H., Herms J. Synapse formation and func-tion is modulated by the amyloid precursor protein //Ibid. – 2006. – 26, № 27. – P.7212–7221.
  20. 20. Rizzoli S.O., Betz W.J. The structural organization ofthe readily releasable pool of synaptic vesicles // Sci-ence. – 2004. – 303, № 5666. – Р.2037–2039.
  21. Sudhof T.C. The synaptic vesicle cycle: a cascade ofprotein-protein interactions // Nature. – 1995. – 375,№ 6533. – Р.645–653.
  22. Wei L., Yu S.P., Gottron F. et al. Potassium channelblockers attenuate hypoxia- and ischemia-induced neu-ronal death in vitro and in vivo // Stroke.– 2003. – 34,№ 5. – Р.1281– 1286.

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