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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(6): 58-65

Functional activity of hippocampalneurons after short-term oxygen-glucose deprivation in vitro

I.V.Lushnikova.

    A.A.Bogomoletz Institute of Physiology NASU,Kiev, Ukraine



Abstract

The deficiency of oxygen and glucose induces neuronal cell damage and death through multiple cellular and molecular mechanisms. The degree of the damage is thought to depend on the intensity and duration of pathological event. In vivo and in vitro experimental investigations have shown that short-term oxygen-glucose deprivation (OGD) results in the delayed cell destruction. It allows studying of the neurodegenerative mecha- nisms induced by OGD for possible corrections. The aim of the present study was to evaluate the morpho-functional changes in cultured hippocampal neurons after short-term OGD followed by reoxygenation during 1, 4 or 24 h. It is revealed that 20 min OGD has no influence on the cell viability, but metabolic activ- ity, synaptic quantity and activity are decreased at 1 h at 4 h after OGD. These functional characteristics are normalized at 24h. The results expand a concept about different aspects of hippocampal neurons injury under deficiency of oxygen and glu- cose. A suggested experimental model could be used for testing different neuroprotective tools.

Full text: (PDF, in Ukrainian)

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