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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(6): 49-57


Cell proliferation and death ofliver mononuclear cells in immunehepatitis induced by concanavalin aand anti-liver antibodies in mice

N.V. Makogon, S.I.Pavlovich, Т.M. Bryzgina,L.I.Alexyuk, V.S. Sukhina, N.G. Grushka, І.M.Аlexeyeva.

    O.O.Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv


Abstract

Two types of experimental liver failure in mice were investigated to study the immune mechanisms of liver disease: 1) T-cell- mediated injury induced by administration of concanavalin A (ConA) and 2) antibody-mediated injury induced by administra- tion of anti-liver antibodies (ALA, ?-globulin fraction of sera from rabbits immunized with liver tissue). It was established, that both types of liver injury were accompanied by the activa- tion of immune processes in the liver, as shown by the increase of liver mononuclear cell proliferation, estimated using IPO-38 monoclonal antibodies. In contrast to ConA treatment, the im- mune activation under ALA-treatment was also associated with the increase in the percentage of plasma cells and small lymphocytes in liver mononuclear cells. At the same time, an increase in apoptotic and necrotic mononuclear cell death was more pronounced under ConA-treatment. This was accompa- nied by enhanced Fas receptor expression in these cells. Thus, it was shown that in case of T-cell mediated liver injury, the balance between cell proliferation and cell death in mononuclear liver cells was shifted toward the significant increase of apoptotic and necrotic cell death, particularly Fas-mediated apoptosis, while immune processes activation and cell proliferation were more pronounced in the case of antibodies-mediated injury.

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