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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(6): 30-37


Effect of ionising irradiationon outward k+ current in rat aortasmooth muscle cells: the roleof protein kinase c

Kizub I.V., Ivanova I.V., Pavlova O.O., Soloviev A.I.

    Institute of Pharmacology and Toxicology of Academy ofMedical Sciences of Ukraine, Kyiv, Ukraine


Abstract

It is known that ?-irradiation leads to vascular hyperfunction and hypertension development. In this study we tested the hy- pothesis that ionizing irradiation directly affects vascular smooth muscle cells due to damage in outward K+ channel function. The goal of this study was to evaluate the influence of whole- body ionizing irradiation (6 Gy dose) on Ca2+- dependent K+ channels and to clarify a possible involvement of protein kinase C in this process. Experiments were conducted on isolated rat aorta smooth muscle cells using whole-cell patch clamp tech- nique. It has been shown that the basic component of outward K+ current in rat aortic smooth muscle cells is a large conductance Ca2+-activated K+ current (BKCa,). BKCa currents in smooth muscle cells obtained from irradiated animals on the 9th and 30th days post-irradiation demonstrated a significant de- crease of K+-current density amplitudes. Protein kinase C in- hibitor, chelerythrine, effectively restored BKCa current reduced by ionizing irradiation. In conclusion, the results suggest that ?-irradiation suppressed BKCa current in vascular smooth muscle cells, and this effect is mainly due to activation of protein kinase C.

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