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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(5): 46-54

The inhibition of oxidative andnitrosative stresses by ecdysterone as the mechanisms of its cardio- and vasoprotectiveaction at type i diabetes

V.F. Sagach, YU.P. Korkach, A.V. Kotsuruba, O.D. Prysyazhna.

    Ін-т фізіології ім. О.О. Богомольця НАН України;Ін-т біохімії ім. О.В. Палладіна НАН України, Київ



Abstract

The effects of whole body ?-irradiation on large conductance Ca2+ - activated K+ channels (BKCa) function and mRNA ex- pression in rat thoracic aorta smooth muscle cells (SMCs) were studied using combined patch-clamp technique in whole- cell modification and RT-PCR analysis. The stimulation of control SMCs by increasingly depolarized voltage steps showed clearly expressed outward K+ currents in control SMCs. Outward currents in SMCs obtained from irradiated animals on the 9th and 30th days post-irradiation demon- strated a significant decrease of K+ current density ampli- tudes. Paxillin was without effect on irradiated cells on 30th day post-irradiation indicating the absence of conductance through BKCa channels. The results of RT-PCR analysis showed that expression both ?-subunit and ?1-subunit of BKCa channels appears to be considerably diminished on 30th day post-irradiation. It is likely that radiation-induced malfunction functional activity of channels is related with insufficient ex- pression of BKCa structural elements in SMCs. In conclusion, the data obtained clearly demonstrate that decreased of the BKCa channels ?- and ?1-subunit expression in SMCs is a key factor of abnormality in BKCa channels activity. This abnormal- ity may contribute to vasorelaxing force depression following non-fatal whole-body ?-irradiation.

Full text: (PDF, in Ukrainian)

References

  1. СПИСОК ЛІТЕРАТУРИ
  2. Стан здоров’я потерпілого населення України через 20 років після Чорнобильської катастрофи. Статистично-аналітичний довідник у 2-х частинах. – Ч. ІІ. – К.: НДВП «Техмедекол», 2007. – 254 с.
  3. Amberg G.C., Santana L.F. Downregulation of the BK channel ?1-subunit in genetic hypertension // Circulat. Res. – 2003. – 93. – P. 965–971.
  4. Chung S.H., Andersen O.S., Krishnamurthy V.V. Bio- logical membrane ion channels dynamics, structure, and applications series: Biological and medical physics, biomedical engineering. – NY.: Springer Scien- ce+BusinessMedia, 2006. – 658 p.
  5. Ledoux J., Werner M.E., Brayden J.E. et al. Calcium- activated potassium channels and the regulation of vas- cular tone // Physiology. – 2006. – 21. – P. 69–78.
  6. Lu R., Alioua A., Kumar Y. et al. Maxi K channel partners: physiological impact // J. Physiol. – 2006. – 570. – P. 65–72.
  7. Orio P., Rojas P., Ferreira G. et al. New disguises for an old channel: MaxiK channel ?-subunit // News Phisiol. Sci. – 2002. – 17. – P.151–161.
  8. Soloviev A., Tishkin S., Zelensky S. et al. Ionizing alters myofilament calcium sensitivity in vas- cular smooth muscle: potential role of protein kinase C // Amer. J. Regul. Integr. Comp. Physiol. – 2005. – 289. – P. R755–R762.
  9. Soloviev A.I., Stefanov A.V., Tishkin S.M. et al. Saline containing phosphatidilcholine liposomes possess the ability to restore endothelial function damaged resulting from ?-irradiation // J. Phisiol. and Pharm. – 2002. – 53, №. 4. – P. 701–702.
  10. 9. Soloviev A.I., Tishkin S.M., Parshikov A.V. et al. Mechanisms of endothelial dysfunction after ionized radiation: selective impairment of the nitric oxide component of endothelium-dependent vasodilatation // Brit. J. Pharmacol. – 2003. – 138, № 8. – P. 837–844.
  11. 10. Tishkin S.M., Recalov V.V., Ivanova I.V. et al. Ionizing non-fatal whole-body irradiation inhibits Ca2+-depen- dent K+ channels in endothelial cells of rat coronary artery: Possible contribution to depression of endot- helium-dependent vascular relaxation // Int. J. Radiat. Biol. – 2007. – 83, № 3. – P. 161–169.
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