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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(5): 46-54


The inhibition of oxidative andnitrosative stresses by ecdysterone as the mechanisms of its cardio- and vasoprotectiveaction at type i diabetes

V.F. Sagach, YU.P. Korkach, A.V. Kotsuruba, O.D. Prysyazhna.

    Ін-т фізіології ім. О.О. Богомольця НАН України;Ін-т біохімії ім. О.В. Палладіна НАН України, Київ


Abstract

The effects of whole body ?-irradiation on large conductance Ca2+ - activated K+ channels (BKCa) function and mRNA ex- pression in rat thoracic aorta smooth muscle cells (SMCs) were studied using combined patch-clamp technique in whole- cell modification and RT-PCR analysis. The stimulation of control SMCs by increasingly depolarized voltage steps showed clearly expressed outward K+ currents in control SMCs. Outward currents in SMCs obtained from irradiated animals on the 9th and 30th days post-irradiation demon- strated a significant decrease of K+ current density ampli- tudes. Paxillin was without effect on irradiated cells on 30th day post-irradiation indicating the absence of conductance through BKCa channels. The results of RT-PCR analysis showed that expression both ?-subunit and ?1-subunit of BKCa channels appears to be considerably diminished on 30th day post-irradiation. It is likely that radiation-induced malfunction functional activity of channels is related with insufficient ex- pression of BKCa structural elements in SMCs. In conclusion, the data obtained clearly demonstrate that decreased of the BKCa channels ?- and ?1-subunit expression in SMCs is a key factor of abnormality in BKCa channels activity. This abnormal- ity may contribute to vasorelaxing force depression following non-fatal whole-body ?-irradiation.

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© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.