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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(5): 28-39

Prenatal dexamethasoneprevents early and long-lasting neuroendocrine and behavioral effects ofmaternal stress on male offspring

A.G. Reznikov, N.D. Nosenko, L.V. Tarasenko, P.V. Sinitsyn, A.A. Lymareva.

    V.P.Komisarenko Institute of Endocrinology and Metabolism, Kyiv



Abstract

The hypothesis on the mediating role of hypothalamic-pitu- itary-adrenocortical (HPA) hormone secretion in neuroendo- crine, neurochemical and behavioral alterations generated by prenatal stress in male rat offspring was tested in this study with dexamethasone (Dex) used for suppression of HPA stress responses. Pregnant dams were being restrained daily for 1 h over the last week of gestation. In male offspring this resulted in attenuation of sex-specific pattern of the protein fractions (on the 5th postnatal day), steroid aromatase activity (on the 10th postnatal day) in the brain preoptic area, and in a decrease of male copulatory behavior, hypothalamic noradrenaline and plasma corticosterone responses to an acute stress, an increase in HPA responses to noradrenergic stimulation and other ef- fects in adulthood. All those changes were prevented with prenatal Dex in a dose of 0.1 mg/kg b.w. injected 30 min prior to restraining pregnant dams. As such, HPA hormone secre- tion mediates alterations of programming of brain develop- ment induced by prenatal stress.

Full text: (PDF, in Ukrainian)

References

  1. Almeida O.F.X., Canoine V., Ali S. et al. Activational effects of gonadal steroids on hypothalamo-pituitary- adrenal regulation in the rat disclosed by response todexamethasone suppression // J. Neuroendocrinol. – 1997. – 9. – P. 129-134.
  2. Antunes-Rodrigues J., McCann S.M. Chemical stimu- lation of water, sodium chloride and food intake by injection of cholinergic and adrenergic drugs into the third brain ventricle // Proc. Soc. Exp. Biol. Med. – 1970. – 133. – P. 1464-1470.
  3. Balashov Yu. G. Fluorimetric micromethod for corticosteroid determination: comparison with other methods // Fiziol. Zh. SSSR Im. M. Sechenova. – 1990. – 76. – P. 280-283. (Rus).
  4. Dorner G., Gotz F., Docke W.D. Prevention of demas- culinization and feminization of the brain in prenatally stressed male rats by prenatal androgen treatment //Exp. Clin. Endocrinol. – 1983. – 81. – P. 88-90.
  5. Douglas A.J. Central noradrenergic mechanisms under- lying acute stress responses of the hypothalamo-pitu- itary-adrenal axis: adaptation through pregnancy and A.G. Reznikov, N.D. Nosenko, L.V. Tarasenko, P.V. Sinitsyn, A.A. Lymareva
  6. 83ISSN 0201-8489 Фізіол. журн., 2008, Т. 54, № 5 Prenatal dexamethasone prevents lactation // Stress. - 2005. – 8. – P. 5-18.
  7. Dygalo N.N., Kalinina T.S., Naumenko Ye.V. Rats fe- tuses brain catecholaminergic system under the corticosteroid balance disturbances // Bull. Exp. Biol. Med. – 1991. –112. – P. 353-355 (Rus.).
  8. Felszeghy K., Bagdy G., Nyakas C. Blunted pituitary- adrenocortical stress response in adult rats following neonatal dexamethasone treatment // J. Neuroendo- crinol. - 2000. – 12. – P. 1014-1021.
  9. Gerardin D.C.C., Pereira O.C.M., Kempinas W.G. et al. Sexual behavior, neuroendocrine, and neurochemical aspects in male rats exposed prenatally to stress // Physiol. Behav. – 2005. – 84. – P. 97-104.
  10. 9. Harms P., Ojeda S. A rapid and simple procedure for chronic cannulation of the rat jugular vein // J. Appl. Physiol. – 1974. – 36. – P. 391-392.
  11. 10. Holson R.R., Gough B., Sillivan P. et al. Prenatal dex- amethasone or stress but not ACTH or corticosterone alter sexual behavior in male rats // Neurotoxicol. Teratol. – 1995. – 17. – P. 393-401.
  12. Jacobowitz D., Richardson J. Method for the rapid determination of norepinephrine, dopamine and sero- tonin in the same brain region // Pharmac. Biochem. Behav.- 1978. – 8. – P. 515-519.
  13. Kapoor A., Dunn E., Kostaki A. et al. Fetal program- ming of hypothalamo-pituitary-adrenal function: pre- natal stress and glucocorticoids // J. Physiol. – 2006. – 572 (Pt. 1). – P. 31-44.
  14. Keshet G.I., Weinstock M. Maternal naltrexone pre- vents morphological and behavioral alterations induced in rats by prenatal stress // Pharmacol. Biochem. Behav. – 1995. – 50. – P. 413-419.
  15. King S., Laplante D.P. The effects of prenatal mater- nal stress on children’s cognitive development: Project Ice Storm // Stress. - 2005. – 8. – P. 35-45.
  16. Koehl M., Darnaudery M., Dulluc J. et al. Prenatal stress alters circadian activity of hypothalamo-pitu- itary-adrenal axis and hippocampal corticosteroid receptors in adult rats of both gender // J. Neurobiol. – 1999. – 40. – P. 302-315.
  17. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage t // Nature (London). – 1970. – 227. – P. 680-685.
  18. Lowry J.H., Rosebrough N.J., Farr A.L. et al. Protein measurement with the folin phenol reagent // J. Biol. Chem. – 1951. – 193. – P. 263-275.
  19. MacLusky N.D., Philip A., Hulburt C. et al. Estro- gen formation in the developing rat brain: sex differ- ences in aromatase activity during early postnatal life // Psychoneuroendocrinology. – 1985. – 77. – P. 355-361.
  20. 19. Nosenko N.D., Mishunina T.M. Glucocorticoid-in- duced prenatal modification of GABA-ergic regulation of the responsiveness of the hypothalamo-hypophy- seal-adrenocortical system to stress in rats // Neurophysiology. – 2005. – 37. – P. 213-218.
  21. 20. Reznikov A.G., Nosenko N.D. Prenatal stress and sexual differentiation of monoaminergic brain systems // Neurophysiology. – 2001. – 33. – P. 197-206.
  22. Reznikov A.G., Tarasenko L.V. Hormonal protection of gender-related peculiarities of testosterone metabo- lism in the brain of prenatally stressed rats // Neuroendocrinol. Ltts.- 2007. – 28. – P. 671-674.
  23. Reznikov A.G., Nosenko N.D., Tarasenko L.V. Prena- tal stress and glucocorticoid effects on the developing gender-related brain // J. Steroid Biochem. Molec. Biol. – 1999a. – 69. – P. 109-115.
  24. Reznikov A.G., Nosenko N.D., Tarasenko L.V. Early postnatal effects of prenatal exposure to glucocorti-coids on testosterone metabolism and biogenic monoamines in discrete neuroendocrine regions of the rat brain // Compar. Biochem. Physiol. – 2004. – Part C. – 138. – P. 169-175.
  25. Reznikov A.G., Nosenko N.D., Tarasenko L.V. Opio- ids are responsible for neurochemical feminization of the brain in prenatally stressed male rats // Neuroendo- crinol. Ltts. – 2005. – 26. – P. 35-38.
  26. Reznikov A.G., Sinitsyn P.V., Tarasenko L.V. Respon- ses of the Hypothalamic-Pituitary-Adrenal Axis to Noradrenergic and Hormonal Stimulation in Prenatally Stressed Rats // Neurophysiology. – 1999b. – 31. – P. 112-114.
  27. 26 Roseboom T., de Rooij S., Painter R. The Dutch fam- ine and its long-term consequences for adult health // Early Hum. Dev. – 2006. – 82. – P. 485-491.
  28. Roselli C.E., Resko J.A. Androgens regulate brain aromatase activity in adult male rats through a receptor mechanism // Endocrinology. – 1984. – 114. – P. 2183-2189.
  29. Roselli C.E., Cross E., Poonyagariyagorn H.K. et al. Role of aromatization in anticipatory and consumma- tory aspects of sexual behavior in male rats // Horm. Behav. – 2003. – 44. – P. 146-151.
  30. 29. Seckl J.R. Prenatal glucocorticoid and long-term pro- gramming // Eur. J. Endocrinol. – 2004. – 151. – P. U49-U62.
  31. 30. Sinitsyn P.V., Tarasenko L.V., Reznikov A.G. Modifi- cations of reactions of the hypothalamo-hypophyseal- adrenocortical system to noradrenergic and cortico- tropic stimulations in rats subjected to prenatal hy- drocortisone treatment // Neurophysiology. – 2005. – 37. – P. 19-23.
  32. Takahashi L.K., Turner J.G, Kalin N.H. Prolonged stress-induced elevation in plasma corticosterone dur- ing pregnancy in the rat: implications for prenatal stress studies // Psychoneuroendocrinology. – 1998. – 23. – P. 571-581.
  33. Wang C.T., Shui H.A., Huang R.L. et al. Sexual moti- vation is demasculinized, but not feminized, in prena- tally stressed male rats // Neuroscience. – 2006. – 138. – P. 357-364.
  34. Ward O.B., Monaghan E.P., Ward I.L. Naltrexone blocks the effects of prenatal stress on sexual behavior differ- entiation in male rats // Pharmacol. Biochem. Behav. –
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