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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(5): 23-27


Potentiation of c-fosexpression in the spinal cord initiated by the signals from fatigue skeletal muscles

O.I. Pilyavskii, A.V. Maznychenko, V.O. Maisky, O.I. Kostyukov.

    Ін-т фізіології ім. О.О. Богомольця НАН України, Київ


Abstract

Immunohistochemical studies of с-fos expression in the lumbar and neck segments of spinal cord after fatiguing stimulation of the mm. gastrocnemius-soleus or m. trapezius and m. splenius, were carried out on the anaesthetized (chloral-hydrate 400 mg/ kg, i.p.) rats. The patterns of the Fos-immunoreactive neurons distribution in the grey matter of spinal cord in the L4 and L5 segments, as well as the C2 and C3 were similar. The highest number of marked cells was registered in the dorsal horn in the first, fourth and fifth layers of grey matter, i.e. within the areas of termination of high-threshold muscle afferents of group ІІІ and ІV. It is assumed, that the types of afferents could be acti- vated by muscle fatigue-induced metabolites. The signals in- coming to the spinal cord could be involved into the presynaptic inhibition of muscle spindle volleys than resulted in the impairment of motor output performance. This mechanism is substantial for limitation of the force of muscles contraction and prevention the injury of the muscles under the excessive physical loading.

References

  1. СПИСОК ЛІТЕРАТУРИ
  2. Серков Ф.Н. Утомление изолированного мы- шечного волокна // Физиол. журн. СССР – 1949. – XXXV, №1. – С. 53–63.
  3. Сеченов И.М. К вопросу о влиянии раздражения чувствующих нервов на мышечную работу чело- века. – В кн. Сеченов И.М. Избр. Сочинения. – 1. – М., 1953.
  4. Abrahams V.C. Group III and IV receptors of skeletal muscle // Can. J. Physiol. Pharmacol. – 1986. – 64. – P. 509–514.
  5. Alberts B., Bray D., Lewis J. et al. (eds). Molecular biology of the cell, 3rd edn. – New York: Garland, 1994.
  6. Coggeshall R.E. Fos, nociception and the dorsal horn // Prog. Neurobiol. – 2005. – 77. – P. 299–352.
  7. Gandevia S.C. Spinal and supraspinal factors in hu- man muscle fatigue // Physiol. Rev. – 2001. – 81. – P. 1725–1789.
  8. Herdegen T., Leah J.D. Inducible and constitutive tran- scription factors in the mammalian nervous system: control of gene expression by Jun, Fos and Krox, and CREB/ATF proteins // Brain Res. Brain Res. Rev. – 1998. – 28. – P. 370–490.
  9. Hoheisel U., Mense S. Long-term changes in discharge behaviour of cat dorsal horn neurones following nox- ious stimulation of deep tissues // Pain. – 1989. – 36. – P. 239–247.
  10. 9. Hoheisel U., Mense S. Response behaviour of cat dor- sal horn neurones receiving input from skeletal muscle and other deep somatic tissues // J. Physiol. – 1990. – 426. – P. 265–280.
  11. 10. Hokfelt T., Rehfeld J.F., Skirboll L. et al. Evidence for coexistence of dopamine and CCK in meso-limbic neurones // Nature. – 1980. – 285. – P. 476–478.
  12. Hsu S.-M., Raine L., Fanger H. Use of avidin-biotin- peroxidase complex (ABC) in immunoperoxidase tech-
  13. ISSN 0201-8489 Фізіол. журн., 2008, Т. 54, № 5 27 niques: a comparison between ABC and unlabelled anti- body (PAP) procedures // J. Histochem. Cytochem. – 1981. – 29. – P. 577–580.
  14. Kalezic I., Bugaychenko L.A., Kostyukov A.I. et al. Fatigue-related depression of the feline monosynaptic gastrocnemius-soleus reflex // J. Physiol. (Lond.). – 2004. – 556. – P. 283–296.
  15. Kostyukov A.I., Bugaychenko L.A., Kalezic I. et al. Effects in feline gastrocnemius-soleus motoneurones induced by muscle fatigue // Exp. Brain Res. – 2005. – 163. – P. 284–294.
  16. Kostyukov A.I., Kalezic I., Serenko S.G., et al. Spread- ing of fatigue-related effects from active to inactiveparts in the medial gastrocnemius muscle of the cat // Eur. J. Appl. Physiol. – 2002. – 86. – P. 295–307.
  17. Lanteri-Minet M., de Pommery J., Herdegen T. et al. Differential time course and spatial expression of Fos, Jun, and Krox-24 proteins in spinal cord of rats under- going subacute or chronic somatic inflammation // J. Comp. Neurol. – 1993. – 333. – P. 223–235.
  18. Marsden C.D., Meadows J.C., Merton P.A. “Muscular wisdom” that minimizes fatigue during prolonged effort in man: peak rates of motoneuron discharge and slow- ing of discharge during fatigue // Adv. Neurol. – 1983. – 39. – P. 169–211.
  19. Mense S. Nociception from skeletal muscle in relation to clinical muscle pain // Pain. – 1993. – 54. – P. 241–289.
  20. Paxinos G., Watson C. The Rat Brain in Stereotaxic Coordinates. – San Diego: Acad. Press, 1997.
  21. 19. Pilyavskii A.I., Maisky V.A., Kalezic I. et al. c-fos expression and NADPH-diaphorase reactivity in spi- nal neurons after fatiguing stimulation of hindlimb muscles in the rat // Brain Res. – 2001. – 923. – P. 91–102.
  22. 20. Pilyavskii A.I., Maznychenko A.V., Maisky V.A. et al. Capsaicin-induced effects on c-fos expression and NADPH-diaphorase activity in the feline spinal cord // Eur. J. Pharmacol. – 2005. – 521. – P. 70–78.
  23. Williams C.A., Loyd S.D., Hampton T.A., Hoover D.B. Expression of c-fos-like immunoreactivity in the fe-line brainstem in response to isometric muscle con- traction and baroreceptor reflex changes in arterial pres- sure // Brain Res. – 2000. – 852. – P. 424–435.

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