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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(4): 30-36


Immunoregulatory role of mesenchymal stem cells in bone reparationprocesses

D.A. Zubov.

    Institute of Genetic and Regenerative Medicine, Kiev


Abstract

Bone marrow contains mesenchymal stem cells (MSC) includ- ing osteoblast progenitor cells. When cultured under conditions promoting an osteoblastic phenotype, MSC proliferate to form colonies that produce alkaline phosphatase and, subsequently, a mature osteoblastic phenotype. Transplantation of cultured au- tologous MSC to patients with non-healing bone fractures gives a good result leading to complete bone fracture consolidation. The aim of the study is to determine a quantitative production of IL-1?, IL-2, IL-4, IL-6, IL-8 and TNF-? by cultured uncom- mitted and committed osteogenic MSC. The results showed that the cytokine profile consisting of IL-1?, IL-2, IL-4, IL-6, IL-8 and TNF-? is secreted by cultured MSC. The secretion of IL- 1? and IL-2 by cultured MSC together with hyper production of IL-6 (up to 276,5 pg/ml, р<0,05) and IL-8 (up to 106,6 ng/ml, р<0,05) by osteoinducted MSC are firstly shown. The immunoregulatory role of transplanted autologous cells in in- flammation and own bone reparation processes during post- traumatic bone fracture healing is highlighted. In conclusion, the data obtained allow examining of cultured autologous MSC as effective activators of bone resorption, inflammation and some immunological reactions in the process of altered osteoreparation.

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