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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(3): 52-56

Remodeling of ammon’shorn during the first two weeks of experimental diabetes development

Y.V. Lebed, M.A. Orlovsky, O.M. Tsupikov, T.A. Pivneva, G.G. Skibo.

    O. O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv;



Abstract

It is known that long-term diabetes mellitus causes hippocampal dysfunction, however, early events leading to diabetes-related impairments of hippocampal tissue remain obscure. The present study was performed to examine temporal and spatial patterns of neuronal damage and astrogliosis in hippocampal CA1-C3 areas during the early stage of streptozotocin-induced diabetes in rats. NeuN and GFAP immunohistochemistry was used to visualize neurons and glial cells. Immunopositive cells were counted in hippocampal CA1-CA3 areas at days 3, 7 and 14 of diabetes development using confocal Olympus FV1000 microscope. Significant decrease in the number of neurons in CA2 area was observed in diabetic rats at day 3. In contrast, in CA1 and CA3 areas NeuN-positive cell count started to decrease later being at day 7, correspondingly, by 7 and 9 % lower than that in the control. This trend developed further till day 14, when the number of neurons in CA1 and CA3 areas was, respectively, 20.3 and 18.1 % smaller as compared with the control. These changes were accompanied by astrogliosis: the number of astrocytes in pyramidal cell layer was increased significantly in all examined time-points. Thus, our study demonstrates that streptozotocin-induced diabetes is associated with early neurodegeneration in Ammon’s horn. It suggests that clinically relevant cognitive deficits development in diabetic patients starting from the early stage of the disease.

Full text: (PDF, in Ukrainian)

References

  1. Amrani A., Durant S., Throsby M. et al. Glucose homeostasis in the nonobese diabetic mouse at the prediabetic stage // Endocrinology. – 1998. – 139, №3. – P.1115–1124.
  2. Anders J.J., Hurlock J.A. Transplanted glial scar im- pedes olfactory bulb reinnervation // Exp. Neurol. –1996. – 142, №1. – P.144–150.
  3. Artola A., Kamal A., Ramakers G.M. et al. Diabetes mellitus concomitantly facilitates the induction of long- term depression and inhibits that of long-term poten- tiation in hippocampus // Eur.J.Neurosci. – 2005. –22, №1. – P.169–178. {02.05.01}
  4. Biessels G.J., van der Heide L.P., Kamal A. et al. Age-ing and diabetes: implications for brain function // Eur. J. Pharmacol. – 2002. – 441, №1–2. – P.1–14.
  5. Collombet J.M., Masqueliez C., Four E. et al. Early reduction of NeuN antigenicity induced by soman poisoning in mice can be used to predict delayed neuronal degeneration in the hippo- campus // Neurosci. Lett. – 2006. – 398, №3. – P.337–342.
  6. Fawcett J.W., Asher R.A. The glial scar and central nervous system repair // Brain Res. Bull. – 1999. – 49, №6. – P.377–391.
  7. Fidler P.S., Schuette K., Asher R.A. et al. Comparingastrocytic cell lines that are inhibitory or permissive for axon growth: the major axon-inhibitory proteoglycan is NG2 // J. Neurosci. – 1999. – 19, №20. – P.8778–8788.
  8. Fuchs E., Weber K. Intermediate filaments: structure, dynamics, function, and disease // Annu. Rev. Biochem. – 1994. – 63. – P.345–382.
  9. 9. Gomes-Leal W., Corkill D.J., Freire M.A. et al. Astrocytosis, microglia activation, oligodendrocyte degeneration, and pyknosis following acute spinal cord injury // Exp. Neurol. – 2004. – 190, №2. – P.456–467.
  10. 10. Kamal A., Biessels G.J., Ramakers G.M. et al. The effect of short duration streptozotocin-induced diabetes mellitus on the late phase and threshold of long-term potentiation induction in the rat // Brain Res. – 2005. – 1053, №1–2. – P.126–130.
  11. Lambert K.G., Gerecke K.M., Quadros P.S. et al. Ac- tivity-stress increases density of GFAP-immuno- reactive astrocytes in the rat hippocampus // Stress. –2000. – 3, №4. – P.275–284.
  12. Lopez-Figueroa M.O., Itoi K., Watson S.J. Regulation of nitric oxide synthase messenger RNA expression in the rat hippocampus by glucocorticoids // Neuro- science. – 1998. – 87, №2. – P.439–446.
  13. Magarinos A.M., McEwen B.S. Experimental diabetes in rats causes hippocampal dendritic and synaptic reorganiza- tion and increased glucocorticoid reactivity to stress // Proc. Natl. Acad. Sci. U. S. A. – 2000. – 97, №20. – P.11056–11061.
  14. McKeon R.J., Jurynec M.J., Buck C.R. The chondroitin sulfate proteoglycans neurocan and phosphacan are ex- pressed by reactive astrocytes in the chronic CNS glial scar // J. Neurosci. – 1999. – 19, №24. – P.10778–10788.
  15. Mullen R.J., Buck C.R.,Smith A.M. NeuN, a neuronal specific nuclear protein in vertebrates // Development. – 1992. – 116, №1. – P.201–211.
  16. Prikryl R. [Cognitive functions impairment in diabetes mellitus patient] // Cas. Lek. Cesk. – 2007. – 146, №5. – P.434–437.
  17. Revsin Y., Saravia F., Roig P. et al. Neuronal and astroglial alterations in the hippocampus of a mouse model for type 1 diabetes // Brain Res. – 2005. – 1038, №1. – P.22–31.
  18. Saravia F.E., Revsin Y., Gonzalez Deniselle M.C. et al. Increased astrocyte reactivity in the hippocampus of murine models of type 1 diabetes: the nonobese dia- betic (NOD) and streptozotocin-treated mice // Ibid. –2002. – 957, №2. – P.345–353.
  19. 19. Silver J., Miller J.H. Regeneration beyond the glial scar / / Nat. Rev. Neurosci. – 2004. – 5, №2. – P.146–156.
  20. 20. Swaab D.F., Bao A.M., Lucassen P.J. The stress systemin the human brain in depression and neurodegeneration // Ageing Res. Rev. – 2005. – 4, №2. – P.141–151.
  21. Trudeau F., Gagnon S. ,Massicotte G. Hippocampal synaptic plasticity and glutamate receptor regulation: influences of diabetes mellitus // Eur.J.Pharmacol. –2004. – 490, №1–3. – P.177–186.
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