Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(2): 82-89


The stress generatedpotential in rats. femur at the different modes of mechanical loading

O.M. Levashov, B.A. Berezovskii, M.I. Levashov, S.L. Saphonov.

    Ин-т физиологии им. А.А. Богомольца НАН Украины, Киев


Abstract

It was studied the stress generated potential (SGP) of moist femur bones of 24 Vistar rats-males in normo- and hypokine- sia conditions. The mechanical loading on bone was evened 30-50 and 100% from animal body mass. The mechanical pres- sure was carried out in axial direction. The rats stay in hard hypokinesia for 28 days. In all experiments Ag-AgCl with a salt bridge from 0,9% NaCl in 2% agar were utilized (Cоchran G.V. et al., 1989). It was set that amplitude of electric poten- tial which arises up in bones at the mechanical loading depends on the level of this loading. However there is a certain opti- mum of the mechanical loading which provides the maximal increase of SGP. This optimum is in the range of physiology level of loadings. At development of hypokinetic osteopenia SGP diminished most substantially in the range of physiology level loadings also. It may be one of reasons of low efficiency of the physical loadings at development age-old osteopenia and slow renewal of bone tissue after hypokinesia.

References

  1. СПИСОК ЛИТЕРАТУРЫ
  2. Beck D.R., Qin Y.X., NcLeod K.J., Otter M.W. On relationship between streaming potential and strain in an in vivo bone preparation//Calcif. Tissue Int. –2002. – 71(4). – P.335–343.
  3. Chilberg P.D., Sale D.G., Webber C.E. Exercise and bone mineral density//Sports Med. – 1995. – 19(2). – P.103–122.
  4. Cochran G.V.B., Dell D.G., Palmiery V.R. et al. An improved desing of ekectrodes for measurement of streaming potentials on wet bone in vitro and in vivo//J. Biomechanics. – 1989. – 22(6/7). – P.745–750.
  5. Cochran G.V., Wu D.D., Lee B.Y. et al. Streaming po- Потенциал нагрузки бедренной кости
  6. ISSN 0201-8489 Фізіол. журн., 2008, Т. 54, № 2 89 tentials in gap osteotomy callus and adjacent cortex. A pilot study//Clin. Orthop. Relat. Res. – 1997. – 337. – P.291–301.
  7. Fukuda S., Iida H. Age related changes in bone mineral cross-sectional area and the strength of long bones in the hind limbs and first lumbar vertebra in female Wistar rats//J. Vet. Med. Sci. – 2004. – 66(7). – P.755–760.
  8. Goodship A.E., Cunningham J.L., Kenwright J. Straine rate and timing of stimulation in mechanical modula- tion of fracture healing//Clin. Orthop. Relat. Res. – 1998. – 355, Suppl. – P.105–115.
  9. Gu W.Y. Mao X.G., Rawlins B.A. et al. Streaming po- tential of human lumbar annulus fibrosus is anisotropic and affected by disc degeneration //J. Biomech. –1999. – 32(11). – P.1177–1182.
  10. Guzelsu N., Walsh W.R. Streaming potential of intact wet bone//J. Biomech. –1990. – 23(7). – P.673–685.
  11. 9. Hastings G.W., Mahmud F.A. Electrical effects in bone/ //J. Biomach. Eng. –1998. – 10(6). – P.515–521.
  12. 10. Legare A., Garon M., Guardo R. et al. Detection and analysis of cartilage degeneration by spatially resolved streaming potential//J. Orthop. Res. – 2002. – 20(4). – P.819–826.
  13. Li D., Chen H., Wang Z. Mechanical response numerical analysis of bone tissue based on liquid saturated biphasic porous medium model //Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. – 2004. – 21(3). –P.381–386.
  14. MacGinitie L.A., Seioz K.G., Otter M.V., Cochran G.V. Streaming potential measurements at low ionic concentrations reflect bone microstructure//J. Biomech. – 1994. – 27(7). – P.969–978.
  15. Mak A.F., Zhang J.D. Numerical simulation of stream- ing potentials due to deformation-induced hierarchical flows in cortical bone//J. Bimech. Eng. – 2001. –123(1). – P. 66–70.
  16. McDonald F. Electrical effects at the bone surface// Europ. J. Orthods. –1993. – 15. – P.175–183.
  17. Nanaka S.V., Sun H.B., Yokota H. Bone formation induced by novel form of mechanical loading on joint tissue//Biol. Sci. Space. – 2004. – 18(2). – P.41–44.
  18. Otter M., Shoenung J., Williams W.S. Evidence for different sources of stress-generated potentials in wet and dry bone// J. Orthop. Res. – 1985. – 3(3). – P.321–324.
  19. Otter M., Goheen S., Williams W.S. Streaming poten- tials in chemically modified bone//J. Orthop. Res. – 1988. – 6(3). – P.346–359.
  20. Otter M.W., Palmieri V.R., Cochran G.V. Transcorti- cal streming potentials are generated by circulatory pressure gradients in living canine tibia//Ibid. – 1990. – 8(1). – P.119–126.
  21. 19. Otter M.W., Palmieri V.R., Wu D.D. et al. A comparative analysis of streaming potentials in vivo and in vitro//Ibid. – 1992. – 10(7). – P.710–719.
  22. 20. Pollack S.R., Salzstein R., Pienkowski D. The electric double layer in bone and its influence on stress-gener- ated potentials//Calcif. Tissue Int. – 1984. – 36, Suppl 1. – P.77–81.
  23. Qin Y.X., Lin W., Rubin C. The pathway of bone fluid as defined by in vivo intramedullary pressure and streaming potential measurements//Ann. Biomed. Eng. – 2002. – 30(5). – P.693–702.
  24. Walsh W.R., Guzelsu N. Ion concentration effects on bone streaming potentials and zeta potentials// Biomaterials. – 1993. – 14(5). – P.331–336.
  25. Walsh W.R., Guzelsu N. Electrokinetic behavior of intact wet bone: compartmental model//J. Orthop. Res. – 1991. – 9(5). – P.683–692.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.