The gaseous exchange,thermoregulation and antioxidant enzyme activities in aging c57bl/6 mice
N.A. Utko, I.N.pishel, V.V. Bezrukov, KH.K. MuradiaN.
Ін-т геронтології АМН України
Abstract
The distribution type and correlative links between physi-
ological and biochemical indices characterizing functional con-
dition of the systems of gaseous exchange (Vo2 and Vco2),
thermoregulation (body temperature and coefficient of
thermoconductivity) and antioxidant defense have been stud-
ied in 62 young (3-5mo.) and 58 old (23-26 mo.) male C57Bl/
6 mice. The coefficients of variation differed significantly de-
pending on the variable but not the age-group. Mean values of
Vo 2 and Vco2, body temperature and thermoconductivity, but
not activities of the antioxidant enzymes, declined in aging.
Moreover, the activities of catalase, glutathione-peroxidase
and glutathionereductase, i.e. enzymes involved in regulation
of hydrogen peroxide level, increased in aging. The correlations
between Vo2 and Vco2, Vo2 and body temperature or Vo2 and
the liver pH, as well as between the antioxidant enzyme activi-
ties exhibited little age-changes. However, three-dimensional
non-linear models revealed significant age-changes in relations
between the studied variables
References
- СПИСОК ЛІТЕРАТУРИ
- Боровиков В. STATISTICA. Искусство анализа данных на компьютере. Для профессионалов. – СПб: Швидкість газообміну, терморегуляція та активність антиоксидантних ферментів
- ISSN 0201-8489 Фізіол. журн., 2008, Т. 54, № 2 81 Питер, 2003. – 688 с
- Мурадян Х.К., Утко Н.А., Мозжухіна Т.Г. та ін. Коре- ляційна залежність між газообміном, температурою тіла та вмістом мітохондріального білка печінки мишей // Фізіол. журн. – 2002. – 48, № 4. – C. 14–18.
- Фролькис В.В. Регулирование, приспособление и старение. – Л.: Наука, 1970. – 432 с.
- Фролькис В.В., Мурадян Х.К. Старение, эволюция и продление жизни. – К.: Наук. думка, 1992. – 238 c.
- Aebi H. Catalase in vitro // Meth. Enzymol. – 1984. – – P. 121–126.
- Balmagiya T., Rozovsky S.J. Age-related changes in thermoregulation in male albino rats // Exp. Gerontol. – 1983. – 18, №3. – P.199–210.
- Brand M.D. Uncoupling to survive? The role of mito- chondrial inefficiency in ageing // Exp. Gerontol. – 2000. – 35, № 6–7. – P. 811–820.
- Brand M.D., Esteves T.C. Physiological functions of the mitochondrial uncoupling proteins UCP2 and UCP3 Cell Metab. – 2005. – 2, № 2. – P. 85–93.
- 9. Frolkis V.V., Muradian Kh.K. Life span prolongation // Boca Raton: CRC Press, 1991. – 425 p.
- 10. Habig W.H., Pabst M.J., Jakoby W. Glutathione S- transferases //J. Biol. Chem. – 1974. – 249, № 22. – P. 7130–7139.
- Hermes-Lima M., Zenteno-Savin T. Animal response to drastic changes in oxygen availability and physi- ological oxidative stress // Comp. Biochem. Physiol. Toxicol. Pharmacol. – 2002. – 133, №4. – P. 537–556.
- McCord J.M., Fridovich I. Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein) // J. Biol. Chem. – 1969. – 244, № 22. – P. 6049–6055.
- Nubel T., Ricquier D. Respiration under control of uncoupling proteins: Clinical perspective // Horm. Res. – 2006. – 65, № 6. – P. 300–310.
- Paglia D.E., Valentine W.N. Studies on the quantitative and qualitative characterization of erythrocyte glu- tathione peroxidase // J. Clin. Med. – 1967. – 70. – P. 158–169.
- Roos D., Weening R.S., Voetman A.A. et al. Protection of phagocytic leukocytes by endogenous glutathione: Studies in family with glutathione reductase deficiency //Blood. – 1979. – 53. – P.851–866.
- Waud W.R., Rajagopalan K.V. Purification and proper- ties of the NAD+-dependent (type D) and O 2 -· -depen- dent (type O) forms of rat liver xanthine dehydroge- nase // Arch. Biochem. and Biophys. – 1976. – 172, № 2. – P. 354–364
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