Nadph-diaphorase activityand neurovascular coupling in the rat cerebral cortex
O.V. Vlasenko, V.A. Maisky, A.V. Maznychenko, A.I. Pilyavskii.
Він. нац. мед. ун-т ім. М.І. Пирогова;Ін-т фізіології ім. О.О. Богомольця НАН України, Київ
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Abstract
The distribution of NADPH-diaphorase-reactive (NADPH-
dr) neurons and neuronal processes in the cerebral cortex and
basal forebrain and their association with parenchymal vessels
were studied in normal adult rats using NADPH-d histochemical
protocol. The intensely stained cortical interneurons and
reactive subcortically originating afferents, and stained
microvessels were examined through a light microscope at law
(x250) and high (x630) magnifications. NADPH-dr interneu-
rons were concentrated in layers 2–6 of the M1 and M2 areas.
However, clear predominance in their concentration (14 ± 0.8
P<0.05 per section) was found in layer 6. A mean number of
labeled neurons in auditory (AuV), granular and agranular (GI,
AIP) areas of the insular cortex was calculated to reach
12.3±0.7, 18.5 ±1.0 and 23.3±1.7 units per section, respectively
(P<0,05). The distinct apposition of labelled neurons to
intracortical vessels was found in the M1, M2. The order of
frequency of neurovascular coupling in different zones of the
cerebral cortex was as following sequence: AuV (31.2 %,
n=1040) > GI (18.0 %, n=640) > S1 (13.3 %, n=720) > M1 (6.3
%, n=1360). A large number of structural associations between
labeled cells and vessels in the temporal and insular cortex indi-
cate that NADPH-d-reactive interneurons can contribute to
regulation of the cerebral regional blood flow in these areas.
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