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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(1): 3-9


Expression of trpv1 and trpv2receptors mrna in the cultured hippocampal neurons

I.I. Drebot , O.I. Boldyryev , V. E. Dosenko, P.G. Kostyuk.

    Ін-т фізіології ім. О.О. Богомольця НАН України,Київ


Abstract

Expression of mRNA of 1 and 2 type (TRPV1 and TRPV2) vanilloid receptors in the cultured hippocampal neurons was determined. With the use of reverse transcription polymerase chain reaction (RT-PCR) of single neurons (single-cell RT-PCR), was shown the TRPV1 and TRPV2 receptors expression in both neurons and glia cell. With use of real - time (real-time PCR) we determined that the level of these genes expression in different neurons is identical. It was also shown that the level of mTRPV1 in GABAergic neurons was considerably higher, than the level of mTRPV2, while expression of genes in the glutamatergic neurons did not differ. Findings about expression of TRPV1 and TRPV2 allow to suppose that vanilloid receptors can play an important functional role in the hippocampal neurons.

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