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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(6): 46-51

Influence of mitogen-activated protein kinases and transcriptional factor c-Fos on estradiol signal transduction in the rat adrenocorticocytes

O.I. Kovzun.




Abstract

Signal transduction mechanisms mediating estradiol regulatory signals in the adrenal cortex were studied in rats. The effect of estradiol benzoate treatment on corticosteroids secretion, levels of ERK1/2, JNK, p38 mitogen-activated protein kinases, tran- scription factors с-Jun and с-Fos in adrenal cortex were investigated. The level of ERK1/2 was increased 1.7-fold in adrenocortical tissue after injections (3 days, 100 mkg per rat) of estradiol. However, the level of p38 kinase and protein kinase JNK was not changed in these conditions. The tran- scription factor AP-1, which includes c-Fos and c-Jun factors, is involved in realization of estradiol signal transduction. The level of c-Fos protein raised 1.8-fold after estradiol treatment, c-Jun protein was not increased. We conclude that ERK1/2 kinase and transcriptional factor c-Fos mediate fast estrogen signal transduction in adrenocorticocytes

Full text: (PDF, in Ukrainian)

References

  1. Балашов Ю.Г. Флюориметрический микрометод определения кортикостероидов: сравнение с други- ми методами // Физиол. журн. СССР. – 1990. – 76, № 2. – С. 280–283.
  2. Ковзун О.І. Шляхи внутрішньоклітинного перене- сення регуляторних сигналів естрадіолу в корі надниркових залоз людини // Доп. НАН України. – 2006. – № 8. – С. 187–191.
  3. Микоша А.С., Ковзун Е.И., Гринченко Е.Н. Эстрадиол-17? активирует образование кортикосте- роидов и тормозит пролиферацию культивируемых клеток надпочечников свиней // Укр. бiохiм. журн. – 2003. – 75, № 1. – С. 29–32.
  4. Юдаев Н.А., Микоша А.С. Влияние эстрона на биосин- тез гидрокортизона надпочечниками морской свинки in vitro // Биохимия. – 1963. – 28, № 3. – С. 462–466.
  5. Akingbemi B.T. Estrogen regulation of testicular func- tion // Reprod. Biol. Endocrinol. – 2005. – 3:51.
  6. Albanito L., Madeo A., Lappano R. et al. G protein- coupled receptor 30 (GPR30) mediates gene expression changes and growth response to 17beta-estradiol and selective GPR30 ligand G-1 in ovarian cancer cells // Cancer Res. – 2007. – 67, № 4. – Р. 1859–1866.
  7. Aronica S.M., Kraus W.L., Katzenellenbogen B.S. Es- trogen action via the cAMP signalling pathway: Stimu- lation of adenylate cyclase and cAMP-regulated gene transcription // Proc. Natl. Acad. Sci. USA. – 1994. – 91. – P. 8517–8521.
  8. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utiliz- ing the principle of protein-dye binding // Anal. Biochem. – 1976. – 72, № 1–2. – P. 248–254.
  9. 9. Clarke I.J., Tobin V.A., Pompolo S., Pereira A. Effects of changing gonadotropin-releasing hormone pulse fre- quency and estrogen treatment on levels of estradiol receptor-alpha and induction of Fos and phospho- rylated cyclic adenosine monophosphate response element binding protein in pituitary gonadotropes: studies in hypothalamo-pituitary disconnected ewes // Endocrinology. – 2005. – 146, № 3. – Р. 1128–1137.
  10. 10. Cutler G.B.Jr., Barnes K.M., Sauer M.A., Loriaux D.L. Estrogen receptor in rat adrenal gland // Ibid. – 1978. – 102, № 1. – Р. 252–257.
  11. Edwards D.P. Regulation of signal transduction path- ways by estrogen and progesterone // Annu. Rev. Physiol. – 2005. – 67. – Р. 335–376.
  12. Gutzman J.H., Nikolai S.E., Rugowski D.E. et al. Pro- lactin and estrogen enhance the activity of activating protein 1 in breast cancer cells: role of extracellularly regulated kinase 1/2-mediated signals to c-fos // Mol. Endocrinol. – 2005. – 19, № 7. – Р. 1765–1778.
  13. Han H.J., Heo J.S., Lee Y.J. Estradiol-17beta stimu- lates proliferation of mouse embryonic stem cells: involvement of MAPKs and CDKs as well as protooncogenes // Amer. J. Physiol. Cell. Physiol. – 2006. – 290, № 4. – C1067–C1075.
  14. Hennessy B.A., Harvey B.J., Healy V. 17beta-Estra- diol rapidly stimulates c-fos expression via the MAPK pathway in T84 cells // Mol. Cell. Endocrinol. – 2005. – 229, № 1–2. – Р. 39–47.
  15. Kanda N., Watanabe S. 17beta-estradiol enhances the production of granulocyte-macrophage colony- stimulating factor in human keratinocytes // J. Invest. Dermatol. – 2004. – 123, № 2. – Р. 329–337.
  16. Karp G., Maissel A., Livneh E. Hormonal regulation of PKC: estrogen up-regulates PKCeta expression in estrogen-responsive breast cancer cells // Cancer Lett. – 2007. – 246, № 1–2. – Р. 173–181.
  17. Lee S.J., McEwen B.S. Neurotrophic and neuro- protective actions of estrogens and their therapeutic implications // Annu. Rev. Pharmacol. Toxicol. – 2001. – 41. – P. 569–591.
  18. Lepique A.P., Moraes M.S., Rocha K.M. et al. c-Myc protein is stabilized by fibroblast growth factor 2 and destabilized by ACTH to control cell cycle in mouse Y1 adrenocortical cells // J. Mol. Endocrinol. – 2004. – 33, № 3. – P. 623–638.
  19. 19. Lesniewska B., Nowak M., Malendowicz L.K. Sex differences in adrenocortical structure and function. XXVIII. ACTH and corticosterone in intact, gonadec- tomized and gonadal hormone replaced rats // Horm. Metab. Res. – 1990. – 22, № 7. – Р. 378–381.
  20. 20. Lobenhofer E.K., Huper G., Iglehart J.D., Marks J.R. Inhibition of mitogen-activated protein kinase and phosphatidylinositol 3-kinase activity in MCF-7 cells prevents estrogen-induced mitogenesis // Cell Growth Differ. – 2000. – 11. – Р. 99–110.
  21. Lopez A., Torres N., Ortiz V. et al. Characterization and regulation of the gene expression of amino acid transport system A (SNAT2) in rat mammary gland // Amer. J. Physiol. Endocrinol. Metab. – 2006. – 291, № 5. – E1059–Е1066.
  22. Nakao M., Sato B., Koga M. et al. Biochemical and immunological characterization of estrogen binding components in human neoplastic adrenocortical tissues // J. Steroid Biochem. – 1986. – 25, № 1. – Р. 1–9.
  23. Pelletier G. Localization of androgen and estrogen receptors in rat and primate tissues // Histol. istopathol. – 2000. – 15, № 4. – Р. 1261–1270.
  24. Seval Y., Cakmak H., Kayisli U.A., Arici A. Estrogen- mediated regulation of p38 mitogen-activated protein kinase in human endometrium // J. Clin. Endocrinol. Metab. – 2006. – 91, № 6. – Р. 2349–2357.
  25. Vivacqua A., Bonofiglio D., Albanito L. et al. 17beta- estradiol, genistein, and 4-hydroxytamoxifen induce the proliferation of thyroid cancer cells through the g protein-coupled receptor GPR30 // Mol. Pharmacol. – 2006. – 70, № 4. – Р. 1414–1423.
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