Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(6): 34-39

Involvement of voltgedependent potassium channels in rabbit corpus cavernosun responces to oxidative stress

Bouryi V.A., Gourkovska A.V., Sukhanova K.Yu., Sagach V.F.




Abstract

Previously we have demonstrated that oxidative stress produces a complex response of the rabbit corporal smooth muscle cells consisting of transient relaxation followed by contraction. We used 4-AP and TEA, selective blockers for voltage- and Ca2+- dependent K+ channels to investigate possible contribution of these channels in maintaining of basal cavernosal tone as well as in mediating of contractile response caused by oxidative stress. TEA in concentration of 1 mmol/l caused contraction of corporal smooth muscle. Application of hydrogen peroxide (H2O2) in the presence of TEA caused contraction similar to that in control conditions. This argues against involvement of Ca2+-dependent K+ channels in contractile response caused by oxidative stress. On the other hand, contractile response on inhibition of Ca2+- dependent K+ channels suggests their contribution in maintaining of corporal tone. 4-AP in concentration of 5 mmol/l caused con- traction resembling that, evoked by TEA. Thus, voltage-depen- dent similar to Ca2+-dependent K+ channels contribute to corporal tone. In the presence of 4-AP H2O2 induced contraction was essentially decreased. The most probable explanation of this result is that population of channels modulated by 4-AP and H2O2 is common for both factors. We previously reported that H2O2 induced contraction could be inhibited by indometacine. Together these results suggest, that H2O2 induced contraction could be a result of inhibition of 4-AP-sensitive voltage-depen- dent K+ channels that is mediated by metabolite products of arachinoide acid via cyclooxigenase pathway

Full text: (PDF, in Ukrainian)

References

  1. Гурковская А.В., Суханова К.Ю., Бурый В.А., Сагач В.Ф. Реакция гладких мышц пещеристого тела кролика на действие активных форм кислорода. // Тавр. медико-биол. вестн. – 2004 – 7, № 1. – C. 51–54.
  2. Гурковська А.В., Суханова К.Ю., Бурий В.О., Сагач В.Ф. Роль ендотелію в реакціях печеристих тіл, які викликані перекисом водню //Фізіол. журн. –2004. – 50, № 6. – C. 27–31
  3. Barnett A.L. Nitric oxide in the penis: physiology and pathology // J. Urol. – 1997. – 157. – P. 320–324.
  4. Bennani S., Benjelloun S. The endothelium of corpus cavernosum. // J. Gynecol. Obstet. Biol. Reprod. – 1994. – 23, № 7. – P. 757–761.
  5. Fujiwara M., Usui H., Kurahashi K. еt al. Endothelium dependent contraction produced by acetylcholine and relaxation produced by histamine in monkey basilar arteries // J.Cardiovasc.Pharm. –1992. – № 20, (Suppl. 12). – S. 114–116.
  6. Hayabuchi Y., Nakaya Y., Matsuoka S. et al. Hydrogen peroxide-induced relaxation in porcine coronary arteries is mediated by Ca2+-activated K+ channels // Heart Vessels. – 1998. – 13, № 1. – P 9–17.
  7. Ito T., Kato T., Iwama Y. et al. Prostaglandin H2 as an endothelium derived contracting factor and its interaction with endothelium derived nitric oxide // J. Hyperten. – 1991, № 9. – P. 729–736.
  8. Karlsson K. Pharmacokinetics of extracellular– super- oxide dismutase in the vascular system //Free Radic.Biol Med. – 1993. – 14, № 2. – P. 185–190.
  9. 9. Matoba T., Shimokawa H., Nakashima M. et al. Hy- drogen peroxide is an endothelium-derived hyperpo- larizing factor in mice // J.Clin. Invest. – 2000. – 106, № 12. – P. 1521–1530.
  10. 10.Shen J.Z., Zheng X.F., Kwan C.Y. Differential contractile actions of reactive oxygen species on rat aorta: selective activation of ATP receptor by Н2О2 // Life Sci. – 2000. – 66, № 21. – P.291–296.
  11. Sotnikova R. Investigation of the mechanisms under- lying H 2 O 2 -evoked contraction in the isolated rat aorta // Gen.Pharmacology. – 1998– 31, №1.– P. 115–119.
  12. Yang Z.W., Zheng T., Wang J. et al. Hydrogen peroxide induces contraction and raises [Ca2+] i in canine cerebral arterial smooth muscle: participation of cellular sig- nalling pathways // Naunyn Schmiedebergs Arch Pharmacol. – 1999. – 360, №6. – P. 646–653.
  13. Yang Z.W., Zheng T., Zhang A. et al. Mechanisms of hydrogen peroxide-induced contraction of rat aorta // Eur.J.Pharmacol. – 1998– 344, №2–3.– P.169–181.
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.