Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(6): 34-39


Involvement of voltgedependent potassium channels in rabbit corpus cavernosun responces to oxidative stress

Bouryi V.A., Gourkovska A.V., Sukhanova K.Yu., Sagach V.F.



Abstract

Previously we have demonstrated that oxidative stress produces a complex response of the rabbit corporal smooth muscle cells consisting of transient relaxation followed by contraction. We used 4-AP and TEA, selective blockers for voltage- and Ca2+- dependent K+ channels to investigate possible contribution of these channels in maintaining of basal cavernosal tone as well as in mediating of contractile response caused by oxidative stress. TEA in concentration of 1 mmol/l caused contraction of corporal smooth muscle. Application of hydrogen peroxide (H2O2) in the presence of TEA caused contraction similar to that in control conditions. This argues against involvement of Ca2+-dependent K+ channels in contractile response caused by oxidative stress. On the other hand, contractile response on inhibition of Ca2+- dependent K+ channels suggests their contribution in maintaining of corporal tone. 4-AP in concentration of 5 mmol/l caused con- traction resembling that, evoked by TEA. Thus, voltage-depen- dent similar to Ca2+-dependent K+ channels contribute to corporal tone. In the presence of 4-AP H2O2 induced contraction was essentially decreased. The most probable explanation of this result is that population of channels modulated by 4-AP and H2O2 is common for both factors. We previously reported that H2O2 induced contraction could be inhibited by indometacine. Together these results suggest, that H2O2 induced contraction could be a result of inhibition of 4-AP-sensitive voltage-depen- dent K+ channels that is mediated by metabolite products of arachinoide acid via cyclooxigenase pathway

References

  1. Гурковская А.В., Суханова К.Ю., Бурый В.А., Сагач В.Ф. Реакция гладких мышц пещеристого тела кролика на действие активных форм кислорода. // Тавр. медико-биол. вестн. – 2004 – 7, № 1. – C. 51–54.
  2. Гурковська А.В., Суханова К.Ю., Бурий В.О., Сагач В.Ф. Роль ендотелію в реакціях печеристих тіл, які викликані перекисом водню //Фізіол. журн. –2004. – 50, № 6. – C. 27–31
  3. Barnett A.L. Nitric oxide in the penis: physiology and pathology // J. Urol. – 1997. – 157. – P. 320–324.
  4. Bennani S., Benjelloun S. The endothelium of corpus cavernosum. // J. Gynecol. Obstet. Biol. Reprod. – 1994. – 23, № 7. – P. 757–761.
  5. Fujiwara M., Usui H., Kurahashi K. еt al. Endothelium dependent contraction produced by acetylcholine and relaxation produced by histamine in monkey basilar arteries // J.Cardiovasc.Pharm. –1992. – № 20, (Suppl. 12). – S. 114–116.
  6. Hayabuchi Y., Nakaya Y., Matsuoka S. et al. Hydrogen peroxide-induced relaxation in porcine coronary arteries is mediated by Ca2+-activated K+ channels // Heart Vessels. – 1998. – 13, № 1. – P 9–17.
  7. Ito T., Kato T., Iwama Y. et al. Prostaglandin H2 as an endothelium derived contracting factor and its interaction with endothelium derived nitric oxide // J. Hyperten. – 1991, № 9. – P. 729–736.
  8. Karlsson K. Pharmacokinetics of extracellular– super- oxide dismutase in the vascular system //Free Radic.Biol Med. – 1993. – 14, № 2. – P. 185–190.
  9. 9. Matoba T., Shimokawa H., Nakashima M. et al. Hy- drogen peroxide is an endothelium-derived hyperpo- larizing factor in mice // J.Clin. Invest. – 2000. – 106, № 12. – P. 1521–1530.
  10. 10.Shen J.Z., Zheng X.F., Kwan C.Y. Differential contractile actions of reactive oxygen species on rat aorta: selective activation of ATP receptor by Н2О2 // Life Sci. – 2000. – 66, № 21. – P.291–296.
  11. Sotnikova R. Investigation of the mechanisms under- lying H 2 O 2 -evoked contraction in the isolated rat aorta // Gen.Pharmacology. – 1998– 31, №1.– P. 115–119.
  12. Yang Z.W., Zheng T., Wang J. et al. Hydrogen peroxide induces contraction and raises [Ca2+] i in canine cerebral arterial smooth muscle: participation of cellular sig- nalling pathways // Naunyn Schmiedebergs Arch Pharmacol. – 1999. – 360, №6. – P. 646–653.
  13. Yang Z.W., Zheng T., Zhang A. et al. Mechanisms of hydrogen peroxide-induced contraction of rat aorta // Eur.J.Pharmacol. – 1998– 344, №2–3.– P.169–181.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.