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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(6): 34-39

Involvement of voltgedependent potassium channels in rabbit corpus cavernosun responces to oxidative stress

Bouryi V.A., Gourkovska A.V., Sukhanova K.Yu., Sagach V.F.


Previously we have demonstrated that oxidative stress produces a complex response of the rabbit corporal smooth muscle cells consisting of transient relaxation followed by contraction. We used 4-AP and TEA, selective blockers for voltage- and Ca2+- dependent K+ channels to investigate possible contribution of these channels in maintaining of basal cavernosal tone as well as in mediating of contractile response caused by oxidative stress. TEA in concentration of 1 mmol/l caused contraction of corporal smooth muscle. Application of hydrogen peroxide (H2O2) in the presence of TEA caused contraction similar to that in control conditions. This argues against involvement of Ca2+-dependent K+ channels in contractile response caused by oxidative stress. On the other hand, contractile response on inhibition of Ca2+- dependent K+ channels suggests their contribution in maintaining of corporal tone. 4-AP in concentration of 5 mmol/l caused con- traction resembling that, evoked by TEA. Thus, voltage-depen- dent similar to Ca2+-dependent K+ channels contribute to corporal tone. In the presence of 4-AP H2O2 induced contraction was essentially decreased. The most probable explanation of this result is that population of channels modulated by 4-AP and H2O2 is common for both factors. We previously reported that H2O2 induced contraction could be inhibited by indometacine. Together these results suggest, that H2O2 induced contraction could be a result of inhibition of 4-AP-sensitive voltage-depen- dent K+ channels that is mediated by metabolite products of arachinoide acid via cyclooxigenase pathway


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