Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(3): 9-15

Nuclear ion channels of the granulеcells from the dentate gyrus

O.A. Fedorenko, D.E. Duzhyy , S.M. Marchenko.

    Bogomoletz Institute of Physiology, National Academy ofScience of Ukraine, Kyiv



Abstract

The nuclear envelope is a part of the endoplasmic reticulum which surrounds the genetic apparatus of the cell. Using the patch-clamp technique we have investigated ion channels in the membranes of nuclei isolated from the neurons of the dentate gyrus. Our research has shown that there is anionic (329 рS) on the outer and anionic (157 pS) and cationic (179 рS) channels on the inner nuclear membrane. Ion channels in the nuclear envelope of the neurons of the dentate gyrus differ much from ones of the neurons of the cerebellum and CA1 region of the hippocampus. This fact proves that different types of neurons express different sets of ion channels in the nuclear membranes. The physiological role of the ion nuclear channels in the granule cells is not clear but they may be important for the ion balance between the cytoplasm and the lumen of the nuclear envelope as well as endoplasmic reticulum of the cell if the latter represents an adequate model of granule of the some parts of ER.

Full text: (PDF, in Ukrainian)

References

  1. Дужий Д.Є., Федоренко О.А., Марченко С.М. Дослідження іоних каналів внутрішньої ядерної мембрани пірамідних нейронів гіпокампа // Фізіол. журн. – 2006. – 52, №2. – С. 18.
  2. Федоренко О.А., Волкова Т.М., Марченко С.М., Новий катіонний канал ядерної мембрани Т- лімфоцитів // Там само. – №1. – С.17–21.
  3. Assandri R, Mazzanti M. Ionic permeability on iso- lated mouse liver nuclei: influence of ATP and Ca2+ // J. Membr. Biol. – 1997. – 157, №3. – Р.301–309.
  4. Debska G., Kicinska A., Skalska J., Szewezyk A. In- tracellular potassium and chloride channels: an update // Acta вiochimica polonica. – 2001. – 48, №1. – P.137–144.
  5. Draguhn A., Borner G., Beckmann R. et al. Large- conductance cation channels in the envelope of nuclei from rat cerebral cortex // J. Membr. Biol. – 1997. –158, №2. – Р.159–166.
  6. Fedorenko O.A., Dujyy D.E., Marchenko S.M. Ca2+ channels in the nuclear membrane of hippocampal py- ramidal neurons // Acta Рhysiol. – 2006. – 186. – Р.183.
  7. Franco-Obergon A., Wang H., Clapham D.E. Distinct ion channel classes are expressed on the outer nuclear envelope of T- and B-lymphocyte cell lines // Biophys.J. – 2000. – 79. – P.202–214.
  8. Gerasimenko O.V., Gerasimenko J.V., Tepikin A.V., Petersen O.H. ATP-dependent accumulation and ino- sitol trisphosphate- or cyclic ADP-ribose-mediated release of Ca2+ from the nuclear envelope // Cell. – 1995. –80, №3. – Р.439–444.
  9. 9. Guihard G., Proteau S., Payet M.D. et al. Patch-clamp study of liver nuclear ionic channels reconstituted into giant proteoliposomes // FEBS Lettr. – 2000. – 476. – Р.234–241.
  10. 10. Humbert J.P., Matter N., Artault J.C. et al. Inositol 1,4,5-trisphosphate receptor is located to the innernuclear membrane vindicating regulation of nuclearcalcium signaling by inositol 1,4,5-trisphosphate // J.Biol. Chem. – 1996. – 271, № 1. – Р.478–485.
  11. Jentsch T.J., Stein V., Weinreich F., Zdebik A.A. Mo- lecular structure and physiological function of cloride channels // Physiol. Rev. – 2001. – 82. – P.503–568.
  12. Kourie J.I. pH-modulation of chloride channels from the sarcoplasmic reticulum of skeletal muscle // J. Membr. Biol. – 1999. – 167, №1. – Р.73–83.
  13. Marchenko S.M., Yarotskyy V.V., Kovalenko T.N. et al. Spontaneously active and InsP3-activated ion channels in cell nuclei from rat cerebellar Purkinje and granuleneurones // J. Physiol. – 2005. – 565, №15. – Р. 897–910.
  14. Marchenko S.M., Thomas R.C. Nuclear Ca2+ signalling in cerebellar Purkinje neurons //Cerebellum. – 2006. – 5, №1. – Р.36–42.
  15. Matzke M., Aufsatz W., Gregor W. et al. Ion transport- ers in the nucleus? // Plant Physiol. – 2001. – 127. – Р.10–13.
  16. Mazzanti M., DeFelice L.J., Cohn J., Malter H. Ion channels in the nuclear envelope // Nature. – 1990. – 22, № 343(6260). – Р.764–767.
  17. Minn A.J., Velez P., Schendel S.L. et al. Bcl-x(L) forms an ion channel in synthetic lipid membranes // Ibid. – 1997. – 23. – №385(6614). – P.353–357.
  18. Nagasawa M., Kanzaki M., Lino Y. et al. Identifica- tion of a noval chloride channel expressed in the endo- plasmic reticulum, Golgi apparatus and nucleus. //J.Biol.Chem. – 2001. – 276, №23. – Р.20413–20418.
  19. 19. Rousseau E., Michaud C., Lefebvre D. et al. Recon- stitution of ionic channels from inner and outer mem- branes of mammalian cardiac nuclei // Biophys. J. – 1996. –70, №2. – P.703–714.
  20. 20. Tonini R., Ferroni A., Valenzuela S.M. et al. Func- tional characterization of the NCC27 nuclear protein in stable transfected CHO-K1 cells // FASEB J. – 2000. –14, №9. – Р.1171–1178
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.