Individual features of human adapta-tion to intermittent hypoxia: pos-sible role of hif gene polymorphisms
T.V. Serebrovska, O.V. Korkushko, V.B. Shatilo,E.O. Asanov, V.A. Ischuk, E.V. Moiseenko,T.I. Drevytska, I.M. Mankovska
O.O. Bogomolets Institute of Physiology, NationalAcademy of Sciences of Ukraine, Kyiv;Institute of Gerontology, AMS of Ukraine, Kyiv
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Abstract
The majority of the adaptation processes to hypoxia is based
on transcriptional regulation by hypoxia-inducible factors – HIFs.
Recently the allele polymorphism of oxygen-dependent deg-
radation domain of HIF-1? has been described. It consists in
the replacement of cytosine for thymine in 1772 location (С 1772 >Т).
The physiological significance of such replacement is obscure.
In the investigation of 26 healthy elderly subjects (58.5 ± 0.7
yr) we tried to verify whether HIF-1? polymorphism in exon
12 may identify individual features of adaptation to intermit-
tent hypoxia training (IHT) (isocapnic hypoxic rebreathing
technique, 5-min sessions with 5 min rest intervals, 3 times
daily during 10 days). The distribution of HIF-1? genotypes
for С 1772 >Т were studied by using the polymerase chain
reaction and restriction analysis. We detected that all subjects
from the group had С/С genotype. Meanwhile, the broad spec-
trum of adaptive reactions to IHT was observed, from the best
adaptation up to disadaptation. IHT enhanced HVRs in the
range of 167% - 5%, blood malon dialdehyde content varied
from a decrease by 46% up to an increase by 88%, the changes
of superoxide dismutase activity varied from +64% to –56%
etc. These results suggest that the С 1772 >Т polymorphism in
HIF-1? does not contribute to individual peculiarities of ad-
aptation to IHT. Because the activity of HIF-1? is regulated
by multiple steps including the transcriptional level, the effect
of the polymorphism in enother exons on the adaptive reactions
remains to be elucidated.
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