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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(1): 55-61

Characteristics of passiveса2+-transport from myometriumsarcoplasmic reticulum.

Iu.V. Danylovych.

    O.V.Palladin Biochemical Institute NAS of Ukraine, Kyiv



Abstract

With use of model of permeabilizated myocytes from rat uterus and 45Са2+ it is shown, that passive Ca2+ transport from sarco- plasmic reticulum (cation has been preliminary accumulated in ATP-dependent process) is sensitive to 2 mM caffeine, 0,1 mM lidocaine, and also to exogenous Са2+ (it was activated at in- crease in concentration Са2+ from 10-7 up to 10-4 M and was suppressed at 10-3) and рН level (was stimulated by alkalization of medium), along with significant suppression of Mg2+ (0,2 - 4 mM). The analysis of the results received allows us to assume that the model used by us is adequate for studying processes of Са2+ release from sarcoplasmic reticulum, mediated by ryanod- ine receptor channels.

Full text: (PDF, in Ukrainian)

References

  1. Бабич Л.Г., Шлыков С.Г., Борисова Л.А. Энергозави-симый транспорт Са2+ во внутриклеточных структу-рах гладкой мышцы // Биохимия. – 1994. – 69, вып.8. – С. 1218–1229.
  2. Вадзюк О.Б., Костерин С.О. Вплив кофеїну наазидчутливе Mg2+, АТФ – залежне збільшенняфлуоресцентної відповіді тетрацикліну при моделю-ванні акумуляцій іонів Са в МХ // Укр. біохім. журн. –2003. – 75, №6. – С.
  3. Костерин С.А. Транспорт кальция в гладких мыш-цах. – К.: Наук. думка, 1990. – 216 с.
  4. Рубцов А.М., Батрукова М.А. Кальциевые каналы(рианодиновые рецепторы) саркоплазматическогоретикулума: структура и свойства // Биохимия. –1997. – 62, вып. 9. – С. 1091–1105.
  5. Awad S.S., Lamb H.K., Morgan J.M. Differential ex-pression of ryanodine receptor RyR2 mRNA in thenon-pregnant and pregnant human myometrium //Biochem. J. – 1997. – 322. – P. 777–783.
  6. Babich L.G., Burdyga Th.V., Shlykov S.G. Evidencefor the intracellular nonmitochondrial calcium store inuterine smooth muscle cells // Укр. биохим. журн. –1997. – 69, №2. – С. 19–29.
  7. Barata H., Thompson M., Zielinska W. The role ofcyclic-ADP-ribose-signaling pathway in oxytocin-in-duced Ca2+-transients in human myometrium cells //Endocrin. – 2004. – 145, №2. – P. 881–889.
  8. Burgardt R.C., Barhoumi R., Sanborn B.M. Oxytocin-induced Ca2+ responses in human myometrial cells //Biol. Reproduct. – 1999. – 60. – P. 777–782.
  9. 9. Chandhary J., Walia M., Matharu J. Caloxin: a novelplasma membrane Ca2+ pump inhibitor // Amer. J.Physiol. – 2001. – 280. – P.C. 1027–1030.
  10. 10. Danylovych Yu. V., Tugay V.A. Research of processesof the passive Ca2+ transport on a model of intact andpermeabilization smooth muscle cells of the uterus //Укр. біохім. журн. – 2005. – 77, №2. – С. 51.
  11. Izumi H., Garfield R.E., Morishita F. Some mechanicalproperties of skinned fibers of pregnant human myo-metrium // Eur. J. Obstet. Gynecol. Reprod. Biol. –1994. – 56, №1. – P. 55–62.
  12. Kargacin M.E., Kargacin G.J. Direct measurment ofCa2+ uptake and release by the sarcoplasmic reticulumof saponin permeabilized isolated smooth muscle cells// J. Gen. Physiol. – 1995. – 106. – P. 467–484.
  13. Krall J.F., Morin A. The role of cyclic GMP in cellswith the properties of smooth muscle cultured from the rat myometrium // J. Cell Physiol. – 1986. – 129. –P. 250–256.
  14. Laporte R., Hui A., Laher J. Pharmacological modula-tion of sarcoplasmic reticulum in smooth muscle //Pharmacol. Rev. – 2004. – 46, №4. – P. 439–513.
  15. Martin C., Chapman K.E., Thornton S. Changes in theexpression of myometrial ryanodine receptor mRNAsduring human pregnancy // Biochem. and Biophys.Acta. – 1999. – 1451. – P. 343–352.
  16. Martin C., Hyvelin J.-M., Chapman K.E. Pregnant ratmyometrial cells show heterogeneous ryanodine- andcaffeine-sensitive calcium stores // Amer. J. Physiol. –1999. – 277, №46. – P.C. 243–252.
  17. Matthew A., Shmygol A., Wray S. Ca2+ entry, effluxand release in smoth muscle // Biol. Res. – 2004. – 37,№4. – P. 617–624.
  18. Mironneau J., Macrez N., Morel J.L. Identificationand function of ryanodine receptor subtype 3 in non-pregnant mouse myometrial cells // J. Physiol. – 2002. –538, №3. – P. 707–716.
  19. 19. Mollard P., Mironneau J., Amedee T. Electrophysi-ological characterization of single pregnant rat myo-metrial cells in short-term primary culture // Amer. J.Physiol. – 1986. – 19, №1. – P.C. 47–54.
  20. 20. Ross R., Klebanoff S.J. The smooth muscle cell. Invivo synthesis of connective tissue proteins // J. CellBiol. – 1971. – 50. – P. 159–171.
  21. Sanders K.M. Mechanisms of calcium handling insmooth muscles // J. Appl. Physiol. – 2001. – 91. –P. 1438–1449.
  22. Uterine contractility. Mechanisms of control / Ed. R.E.Garfield.: Serano Symposia, USA. – 1990. – 388 p.
  23. Wray S., Kupittayanant S., Smygol A. The physiologi-cal basis of uterine contractility: a short review // Exp.Physiol. – 2001. – 86, №2. – P. 239–246.
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