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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(1): 24-32


Transfer factor of immune reactivityto diphtheria-tetanus anatoxin modu-lates the action of neurotransmittersin intestinal smooth muscle

N.V.Melenevska, M.S.Miroshnichenko,I.B.Phylippov, L.S.Kholodna, M.F.Shuba.

    National University named after T.Shevchenko, Kyiv, UkraineO.O.Bogomolets Physiology Institute of NAS, Kyiv, Ukraine


Abstract

Transfer factor (TF) of immune reactivity (10-5–10-3mg/ml) to diphtheria-tetanus anatoxin modulates slow waves and spon- taneous contractile activity of non-atropinized smooth muscle stripes (SMS) of guinea-pig taenia coli. TF (10-4mg/ml) trans- forms slow waves into stable depolarization and tonic con- traction. After SMS atropinization, the substance acts in the same way. In the presence of methylene blue (10-5M), a guanylatecyclase blocker, FT induces transitory increase of SMS muscle tone, which is followed by their stable relaxation. ATP and UTP, purinoceptors agonists, evoke substantial hy- perpolarization of smooth muscle cells membrane and their relaxation. FT enhances post-inhibitory excitation in SMS. In the presence of acetylcholine (10-5M) FT (10-4mg/ml) trans- forms the inhibitory ATP action on tonic contraction into excitative. This substance (10-5, 10-4 mg/ml) enhances Ca2+ mobilization from ryanodine-sensitive calcium store, inhibits the release of these cations from IP3-sensitive calcium store of sarcoplasmic reticulum. TF demolishes the inhibitory actions of sodium nitroprusside (nitric oxide donor), and noradrenaline in taenia coli smooth muscles.

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