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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(1): 24-32

Transfer factor of immune reactivityto diphtheria-tetanus anatoxin modu-lates the action of neurotransmittersin intestinal smooth muscle

N.V.Melenevska, M.S.Miroshnichenko,I.B.Phylippov, L.S.Kholodna, M.F.Shuba.

    National University named after T.Shevchenko, Kyiv, UkraineO.O.Bogomolets Physiology Institute of NAS, Kyiv, Ukraine



Abstract

Transfer factor (TF) of immune reactivity (10-5–10-3mg/ml) to diphtheria-tetanus anatoxin modulates slow waves and spon- taneous contractile activity of non-atropinized smooth muscle stripes (SMS) of guinea-pig taenia coli. TF (10-4mg/ml) trans- forms slow waves into stable depolarization and tonic con- traction. After SMS atropinization, the substance acts in the same way. In the presence of methylene blue (10-5M), a guanylatecyclase blocker, FT induces transitory increase of SMS muscle tone, which is followed by their stable relaxation. ATP and UTP, purinoceptors agonists, evoke substantial hy- perpolarization of smooth muscle cells membrane and their relaxation. FT enhances post-inhibitory excitation in SMS. In the presence of acetylcholine (10-5M) FT (10-4mg/ml) trans- forms the inhibitory ATP action on tonic contraction into excitative. This substance (10-5, 10-4 mg/ml) enhances Ca2+ mobilization from ryanodine-sensitive calcium store, inhibits the release of these cations from IP3-sensitive calcium store of sarcoplasmic reticulum. TF demolishes the inhibitory actions of sodium nitroprusside (nitric oxide donor), and noradrenaline in taenia coli smooth muscles.

Full text: (PDF, in Ukrainian)

References

  1. Артеменко Д.П., Бурый В.А., Владимирова И.А.,Шуба М.Ф. и др. Модификация метода одинарногосахарозного мостика//Физиол. журн. – 1982. – 14,№ 3. – С.374–380.
  2. Зима А., Белевич А., Цицюра Я., Шуба М. Действиеокиси азота на Са2+- и Са2+-активируемые К+ каналыв гладкомышечных клетках taenia coli морскойсвинки//Физика живого. – 1996. – 4, № 1. – С.67–72.
  3. Лоуренс Х., Эль-Аскари С. Приготовление иочищение фактора переноса// Методы изучения invitro клеточного иммунитета/ Под.ред. Б.Блума,Ф.Глейда. – М., Медицина, 1974. – С.256–272.
  4. Любченко Т.А., Голева О.Г., Холодна Л.С. та ін.Біологічна активність фактора переносу, індуко-ваного бактеріальними антигенами// Мікробіол.журн. – 1997. – 59, № 5. – C.83–100.
  5. Любченко Т.А., Голева О.Г., Холодна Л.С. Факторпереносу як модулятор клітинної відповіді//Биополимеры и клетка. – 1997. – 13, № 5. – 345 c.
  6. Любченко Т.А. Імунологічна активність факторапереносу імунної реактивності індукованогобактеріальними антгенами: Автореф. дис. … канд.біол. наук. – К.,1999. – 15 с.
  7. Позднеев О.К. Медицинская микробиология// Подред. Покровского В.И. – М.: Медицина, 2001. – 324 с.
  8. Позур В.К., Колибо Д.В., Борисов В.А. та ін.Структура і біологічна активність бактеріальнихбіополімерів. – К.: Вид-во Київ. ун-ту, 2003. – 304 с.
  9. 9. Хухо Ф. Нейрофизиология. Основы и принципы. –М.: Мир, 1990. – 283 с.
  10. 10. Aminova G.G. The caecum lymphoid structure inproterm and full term newborns//Morfology. – 2000. –118, № 5. – P.42–45.
  11. Bolton T.B., Prestvich S.A., Zholos A.V. et аl. Excita-tion – contraction coupling in gastrointestinal and othersmooth muscle//Annu. Rev. Physiol. – 1999. – 61. –P.85–115.
  12. Bolton T.B., Gordienko D.V., Pucovsky V. et аl. Calciumevents in smooth muscles and their associated cells//Neurophysiology. – 2003. – 35, № 3/4. – P.183–188.
  13. Comile F., Martin L., Lenoir C. et al. Cooperative exosite –dependent cleavage of synaptobrevin by tetanus toxinlight-chain//J. Biol. Chem. – 1997. – 272, № 6. –P.3459–3464.
  14. Finkenstein A., Oh K.J., Senzel L. The diphtheria toxinchannel-forming T-domain translocates its own NH2-terminal region and the catalityc domain across planarphospholipids blayers// Int. J. Med. Microbiol. – 2000. –290, № 4–6. – P.435–440.
  15. Lammers W.J.E.P., Donck L.V., Schuurkes J.A.J et. allLongitudinal and circumferential spike patches in the caninesmall intestine in vivo //Amer. J. Physiol. Gastrointest.Liver Physiol. – 2003. – 285, № 5. – P. G1014–G1027.
  16. Mitzuta Y., Isomoto H., Takahashi T. Impaired nitrergicinnervation in rat colitis induced by dextran sulfate so-dium// Gastroenterology. – 2000. – 118, № 4. – P.714–723.
  17. Ralevic V., Burnstock G. Receptor for purines andpyrimidines//Pharmacol. Rev. – 1998. – 50, № 3. –P.413–492.
  18. Roland Seiler B.A., Andreas Rickenbacher B.A., SidneyShaw, Bruno M. Balsiger. ?- and ?-adrenergic receptormechanisms in spontaneous contractile activity of ratileal longitudinal smooth muscle// J.Gastroint. Surgery. –2005. – 9, № 2. – Р. 227–235.
  19. 19. Sanders K.M., Ward S.M. Nitric oxide as a mediator ofnonadrenergic noncholinergic neurotransmission//Amer. J.Physiol. (Gastrointest. Liver.Physiol. 25). –1992. – 262, № 3, Pt 1. – P. G379–G329.
  20. 20. Shuba M.P., Vladimirova I.A., Philippov I.B. Mecha-nism of inhibitory action of neurotransmitters onsmooth muscle//Neurophysiology. – 2003. – 35, № 3/4. –P.252–261.
  21. Zholos A.V., Tsytsyura Y.D., Gordienko D.V. et al.Phospholipase C, but not Ins3 or DAG, -dependentactivation of the muscarinic receptor-operated cationcurrent in guinea-pig ileal smooth muscle cells//Brit.J.Pharmacol. – 2004. – 141. – P.23–36.
  22. Zholos A.V., Zholos A.A., Bolton T.B. G-protein-gatedTRP – like cationic channel activated by muscarinicreceptors effect of potential on single-channel gating//J. Gen. Physiol. – 2004. – 123, № 5. – P.581–598.
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