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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(5): 34-40

Morphological featuresof apoptosis in normal and injured cultured rat hepatocytes under thromboxaneВ treatment

N.G. Grushka, G.M. Korniychuk, N.V. Makogon, I.M. Alexeyeva

    O.O. Bogomoletz Institute of Physiology National Academyof Sciences of Ukraine, Kiev



Abstract

Тhromboxanes (TX) are known to have damaging effect on a liver but their influence on the cell death, in particular on hepatocyte apoptosis and its morphological features is not investigated enough. Cell death of the rat hepatocytes was investigated in primary culture by double vital staining with fluorescent nuclear stains Hoechst 33342 and propidium io- dide and by electron microscopy. It was established that exog- enous Tx B2 increases the amount of hepatocytes with early stages of apoptosis - the condensed chromatin and nucleus and cell size reduction. The changes in a percentage of hepato- cytes with morphological features of the late stages of apoptosis - fragmented nuclei and division on apoptotic bodies were not revealed. Tx B2. intensified the carbon tetrachloride action on hepatocyte apoptotic death and increased chromatin condensation. Тх В2 application to hepatocytes injured by chenodeoxycholic acid significantly increased the amount of cells with a final stage of apoptosis.

Full text: (PDF, in Ukrainian)

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