Morphological featuresof apoptosis in normal and injured cultured rat hepatocytes under thromboxaneВ treatment
N.G. Grushka, G.M. Korniychuk, N.V. Makogon, I.M. Alexeyeva
O.O. Bogomoletz Institute of Physiology National Academyof Sciences of Ukraine, Kiev

Abstract
Тhromboxanes (TX) are known to have damaging effect on a
liver but their influence on the cell death, in particular on
hepatocyte apoptosis and its morphological features is not
investigated enough. Cell death of the rat hepatocytes was
investigated in primary culture by double vital staining with
fluorescent nuclear stains Hoechst 33342 and propidium io-
dide and by electron microscopy. It was established that exog-
enous Tx B 2 increases the amount of hepatocytes with early
stages of apoptosis - the condensed chromatin and nucleus
and cell size reduction. The changes in a percentage of hepato-
cytes with morphological features of the late stages of apoptosis
- fragmented nuclei and division on apoptotic bodies were not
revealed. Tx B 2. intensified the carbon tetrachloride action on
hepatocyte apoptotic death and increased chromatin
condensation. Тх В2 application to hepatocytes injured by
chenodeoxycholic acid significantly increased the amount of
cells with a final stage of apoptosis.
References
- Бабак О.Я. Хронические гепатиты. – К.: Блиц- Информ, 1999. – 208 с.
- Буко В.У. Простагландины при алкогольном пораже- нии печени. – Минск: Навука і тэхніка, 1991. – 152 c.
- Фильченков А.А. Современные технологии коли- чественной оценки апоптоза и их применение в экспериментальной и клинической онкологии. – К.: ДИА , 2003. – 76 с.
- Хабатюк Н.Г., Макогон Н.В., Корнійчук Г.М., Алексєєва І.М. Участь циклооксигеназного шляху метаболізму арахідонової кислоти в модуляції апоптозу культивованих гепатоцитів щурів: Зб. наук. праць співробітників КМАПО ім. Н.А. Шупика, – К., 2004. – С.236–240.
- Beauchamp M.H., Martinez-Bermudez A.K., Gobeil F.J еt al. Role of thromboxane in retinal microvascular degeneration in oxygen-induced retinopathy // J. Appl. Physiol. – 2001. – 90, №6. – Р.2279–2288.
- Cai Y., Gong L., Qi X. еt al. Apoptosis initiated by carbon tetrachloride in mitochondria of rat primary cultured hepatocytes // Acta Pharmacologica Sinica. – 2005. – 26, №8. – Р.969–975.
- Horton A.A., Wood J.M. Prevention of thromboxane B2- induced hepatocyte plasma membrane bleb formation by certain prostaglandins and a proteinase inhibitor // Biochim. Biophys. Acta. – 1990. – 1022, № 3. – Р.319–324.
- Lemasters J. Mechanisms of hepatic toxicity. V. Necroapoptosis and the mitochondrial permeability transition: shared pathways to necrosis and apoptosis // Amer. J.Physiol. – 1999. – 276, №1. – P.G1–G6.
- 9. Marinovich M., Flaminio L., Papagni M., Galli C. Stimu- lation of arachidonic acid metabolism by CCl4 in iso- lated rat hepatocytes // Prostaglandins. – 1989. –37, № 1. – P.23–30.
- 10. Nanji A.A., Khettry U., Sadrzadeh S.M., Yamanaka T. Severity of liver injury in experimental alcoholic liver disease. Correlation with plasma endotoxin, prostag- landin E2 leukotriene B2, and thromboxane B2 // Amer. J. Pathology. – 1993. – 142, №2. –P.367–373.
- Okada M., Sagawa T., Tominaga A. et al. Two mecha- nisms for platelet-mediated killing of tumour cells: one cyclo-oxygenase dependent and the other nitric oxide dependent // Immunology. – 1996. – 89, № 1. – Р.158–164.
- Pieper A., Verma A., Zhang J., Snyder S. Poly (ADP- ribose) polymerase, nitric oxide and cell death// TiPS. – 1999. – 20. – P.171–181.
- Seglen P. Preparation of rat liver cells // Methods Cell Biol. – 1976. – 13. –P.29–83.
- Shi J., Aisaki K., Ikawa Y., Wake K. Evidence of hepato- cyte apoptosis after the administration of carbon tetra- chloride // Amer. J.Pathol. – 1998. – 153, №2. – P.515–525.
- Shimizu S., Eguchi Y., Kamiike W. et al. Involvement of ICE family proteases in apoptosis induced by re- oxygenation of hypoxic hepatocytes// Amer. J.Phisiol. – 1996. – 271, № 6. – P. 949–958.
- Tolman K. G. Eicosanoids and the liver // Prostaglandins and other Lipid Mediators. – 2000. – 61. – P.163–174.
- Totsuka E., Sasaki M., Takahashi K. et al. The effects of intraportal prostaglandin E1 administration on he- patic ischemia and reperfusion injury in dogs // Sur- gery Today. – 1995. –25, №5. – P.421–428.
|