Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(1): 71-77


Anti-idiotypic antibodies as a phisiological mechanism ofcofactor- independent antiphospolipid antibodies supression in women undergoing IVF

B.V. Donskoy, V.P. Chernyshov

    Institute of Pediatrics, Obstetrics and Gynecology, AcademyMedical Sciences of Ukraine, Kyiv


Abstract

Antiphospholipid antibodies (АPА) and anti-idiotypic antiphospholipid antibodies (AiAPA) were studied in 148 women subjected to IVF. АPА (IgG аCL, аPS,) levels in serum have been defined. Sera IgG fraction was also examined for the presence of AiAPA by three different methods: 1) binding of AiAPA with mouse monoclonal cofactor-independent APA (mAPA) immobilized on plate; 2) AiAPA neutralization of human affinity isolated APA and 3) mAPA binding with phos- pholipids. Significant difference in AiAPA levels between APA+ and APA- women subjected to IVF have been found. The mean level of AiAPA was lower in APA+ women subjected to IVF than in APA- women from the same group (p<0,05). IV infusion of Ig decreased the APA level significantly as well as increased the AiAPA level in APA+ women subjected to IVF. Ig applica- tion to incubation in vitro results in decrease of APA levels in serum. Results of this study confirmed possibility of AiAPA participation in down regulation of cofactor-independent APA production in women undergoing to IVF.

References

  1. Чернышов В.П., Дахно Ф.В., Донской Б.В., Сиренко В.Ю. Антифосфолипидные антитела и результаты оплодотворения in vitro // Здоровье женщины. – 2004. – 2, №18. – С. 137–139.
  2. Caruso A., Carolis S.D., Simone N.D. Antophospholipid antibodies in obstetrics: new complexites and sites of ac- tion // Hum. Reprod. Update. – 1999. – 5. – P. 267–276.
  3. Donskoy B.V., Chernyshov V.P., Vodyanic M.A. Antiphospholipid antibodies and antiidiotypic antiphos- pholipid antibodies in women undergoing IVF/ET // Amer.J. Reprod. Immunol. – 2004. – 51, №6 – Р. 492.
  4. Geva E., Yaron Y., Lessing J.B. Circulating autoim- mune antibodies may be responsible for implantation failure in in vitro fertilisation // Fertil. and Steril. –1994. – 62. – P. 802–806.
  5. Gleicher N. Antiphospholipid antibobies and reproduc- tive failure // Hum. Reprod. – 1997. – 12. – P. 13–16.
  6. Greaves M. Antiphospholipid antibodies and throm- bosis // Lancet. – 1999. – 353. – P. 1348–1353.
  7. Hojnik M., Gilburt B., Ziporen L. ACA in infection are heterogenous in their dependency on b2GP1 // Lu- pus. – 1994. – 3. – P. 515–521.
  8. Kandiah D. A., Sali A., Sheng E.J. Current Insights into “Antiphospholipid” syndrome: Clinical, Immuno- logical, and Molecular Aspects // Adv. Immunol. – 1998. –70. – P. 507–562.
  9. 9. Krause M., Blank Y., Levi T., Koike I. Anti-idiotype immunomodulation of experimental anti-phospholipid syndrome via effect on Th1/Th2 expression // Clin.Exp. Immunol. – 1999. – 117. – P. 190–197.
  10. 10. Kutteh W. H.., Rote N.S., Silver R. Antiphospholipid antibodies and reproduction: the Antiphospholipid antibody syndrome // Amer. J.Reprod. Immunol. –1999. – 41. – P. 133–151.
  11. Landenberg C., Lackner K.J., Landenberg P. et al. Antiphospholipid antibodies, Isolation and charac- terization of two human monoclonal APL IgG frompatients with autoimune disease // J.Autoimmun. –1999. – 13. – P. 215–223.
  12. Larranaga G.F., Forastiero R.R., Carreras L.O. Differ- ent types of APA in AIDA a comparison with siphilis and APS // Thromb Res. – 1999. – 96. – P. 19–25.
  13. Levine J.S., Subang R., Koh J.S., Rauch J. Induction of APA by b2-glicoprotein I bound to apoptic thimocites // J.Autoimmun. – 1999. – 4. – Р. 124–126.
  14. McIntyre J.A., Wagenknecht D.R., Triplett D.A. De- tection of APA in heat inactiveted normal human sera // Thromb. Res. – 1993. – 69. – P. 489–490.
  15. Shan H., Goldman J., Cunto G. Heterogeneity of APA and anti-endothelial cell antibobies // J.Autoimmun. –1998. – 11. – P. 651–660.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.