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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(1): 49-61


Identification of Е-4031-sensitive potassium current component (IKr)in cardiodifferentiated murine P19 embrionic carcinoma cells

A.V. Sotkis, Lazarenko R.M., Boldyrev O.I., Voitychuk O.I., Dosenko V.Je., Shuba Ia.M.

    О.О. Bogomolets Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv


Abstract

Pluripotent mouse P19 embryonic carcinoma cells represent a convenient in vitro model for studying various aspects of cardiac differentiation. Here by using whole-cell patch-clamp recording we have identified the rapid delayed rectifier K+ current, IKr, in P19 cell induced to differentiate into cardiac phenotype by DMSO (1%). Cardiac differentiation was confirmed by the appearance of spontaneously beating cells, their morphological features, ultrastructural clusterization of mitochondria around contraction elements, expression of cardiac actin mRNAs and 06ISSN 0201-8489 Фізіол. журн., 2006, Т. 52, № 1 MLC2v, and by the presence of inward sodium and calcium currents. IKr was isolated based on the sensitivity to the spe- cific blocker, Е-4031, which at concentration of 1 мM blocked more than 50% of the total outward K+ current. However, in contrast to IKr in native cardiac myocytes and in heterologous systems expressing IKr-carrying ERG1 potassium channel, Е-4031-sensitive K+ current in cardiac-like P19 cells lacked characteristic inward rectification, suggesting specific regulation and/or subunit composition of endogenous ERG1-based channel in these cells. Establishing the reason(s) for this phe- nomenon will advance the understanding of the mechanisms of IKr-channel rectification. Cardiac-differentiated P19 cells might also be useful for studying pharmacological modulation of IKr, which is recognized target for cardiotoxic side effects of numerous drugs

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