Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(1): 17-21

New cation channel of the T-lymphocytenuclear membrane

O.A. Fedorenko, T.M. Volkova, S.M. Marchenko

    О.О. Bogomolets Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv


There are little data on the properties of ion channels in the inner nuclear membrane of lymphocytes, though the transport system between cytoplasm and karyoplasm is of a great importance for a complex genetic regulation in these cells. Using the patch-clamp technique we have investigated ion channels of the inner membrane of nuclei isolated from cultured Т-lymphoblasts. Our research has shown that there are anionic (370 рS) and cationic (152 рS) channels on the inner nuclear membrane. The latter were characterized by fast kinetics and rapid fluctuations; they had high open probability and were inactivated at large negative potentials. These channels were permeable for K+ and Na+, but impermeable for Cl-. The physi- ological role of the channels is not clear, but they may play an important role in the ion balance between the cytoplasm and the lumen of the endoplasmatic reticulume of the cell.


  1. Bortner C.D., Cidlowski J.A. Uncoupling cell shrinkage from apoptosis reveals that Na+ influx is required for volume loss during programmed cell death // J. Biol.Chem. – 2003. – 278. – P. 39176–39184.
  2. Сlements L, Manilal S, Love DR, Morris GE. Direct interaction between emerin and lamin A // Biochem. Biophys. Res. Commun. – 2000. – 267. – P. 709–714.
  3. Franco-Obergon A., Wang H., Clapham D.E. Distinct ion channel classes are expressed on the outer nuclear envelope of T- and B-lymphocyte cell lines // Biophys.J. –2000. – 79. – P. 202–214.
  4. Gerasimenko O.V., Gerasimenko J.V., Tepikin A.V., Petersen O.H. ATP-dependent accumulation and inositol trisphosphate- or cyclic ADP-ribose-mediated release of Са2+ from the nuclear envelope // Cell. –1995. – 80. – P. 439–444.
  5. Marchenko S.M., Yarotsky V.V., Kovalenko T.N. еt al. Spontaneously active and InsP 3-activated ion channels in cell nuclei from rat cerebellar Purkinje and granule neurons // J Physiol. – 2005. – Mar 17.
  6. Mazzanti M., Bustamante J.O., Oberleithener H. Elec- trical dimension of the nuclear envelope // Physiol. Reviews. – 2001. – 81, №1. – Р. 1–19.
  7. Paul W.E., Seder R.A. Lymphocyte response and Cytokines // Cell. – 1994. – 76. – P. 241–251.
  8. Picard L., Cote K., Teijeira J. et al. Sarcoplasmic reticulum K(+) channels from human and sheep atrial cells display a specific electro-pharmacological pro-file// J. Mol. Cell. Cardiol. – 2002. – 34. – P.1163–1172.
  9. 9. Worman H.J. Components of the nuclear envelope and their role in human disease // Novartis Found Symp. –2005. – 264. – Р. 35–50.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.