Allelic polymorphism of largemultifunctional proteases and itsfunctional meaning
V.E. Dosenko, D.A. Mikhalchuk, V.Yu. Zagoriy,N.V. Haitovich, A.A. Moibenko.
O.O.Bogomoletz Institute of Physiology National Academy ofScience of Ukraine, KyivKyiv State University
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Abstract
Proteasomal activity in isolated monocytes from subjects with
different variants of large multifunctional proteases genes -
LMP2 (Arg60 His allelic polymorphism) and LMP7
(Lys145 Gln allelic polymorphism) was determined.
Trypsin-like activity of proteasome was 1.6-times (P=0.19)
higher at Arg/Arg genotype comparing to His/His genotype,
and 2-fold lower than at Arg/His genotype. The highest
chymotrypsin-like activity of proteasome was observed in
heterozygotes (on 29.7% higher comparing to Arg/Arg geno-
type, Р=0.43) and lowest in homozygotes His/His (on 29.7%
less comparing to Arg/Arg genotype, Р=0.40). Level of RNA
expression in isolated monocytes detetermined by use of RT-
PCR did not differed significantly in subjects with different
genotypes. Data obtained indicate that LMP2 allelic poly-
morphism impact peptidase activity of immunoproteasome.
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