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The comparative study of the newfluorine-containing guanidine-derivate compounds and pinacidil’sinfluence on the contractile func-tion of a urinary bladder in vitro
I. Limarenko
Institute of Pharmacology and Toxi

Abstract
The effects of the new fluorine-containing guanidine-derivate
compounds on bladder contractile function were examined on
isolated bladder strips in vitro. Female rats (250-350 g) were
used. The bladder was removed and placed in the room
temperature PSS of the following composition (mM): NaCl
(118.4), KCl (4.7), CaCl 2 (2.5), MgSO4 (1.2), KH 2PO 4(1.2),
NaHCO3 (24.9), and D-glucose (11.1) gassed with O2-CO2,
95%-5%. The base of the bladder was then cut into strips 4-to
5-mm wide and 10-mm long. Then strips were pretensioned
(0.25-0.5 g) and after 30min of equilibration were contracted
with an additional 60mM KCl and again were allowed to
equilibrate. Compounds were administered directly into the
tissue bath. All compounds produced a concentration-
dependent relaxation of isolated rat bladder strips.
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© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2025.
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