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The comparative study of the newfluorine-containing guanidine-derivate compounds and pinacidil’sinfluence on the contractile func-tion of a urinary bladder in vitro
I. Limarenko
Institute of Pharmacology and Toxi
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Abstract
The effects of the new fluorine-containing guanidine-derivate
compounds on bladder contractile function were examined on
isolated bladder strips in vitro. Female rats (250-350 g) were
used. The bladder was removed and placed in the room
temperature PSS of the following composition (mM): NaCl
(118.4), KCl (4.7), CaCl 2 (2.5), MgSO4 (1.2), KH 2PO 4(1.2),
NaHCO3 (24.9), and D-glucose (11.1) gassed with O2-CO2,
95%-5%. The base of the bladder was then cut into strips 4-to
5-mm wide and 10-mm long. Then strips were pretensioned
(0.25-0.5 g) and after 30min of equilibration were contracted
with an additional 60mM KCl and again were allowed to
equilibrate. Compounds were administered directly into the
tissue bath. All compounds produced a concentration-
dependent relaxation of isolated rat bladder strips.
References
- Беленький М.Л. Элементы количественной оценкифармакологического эффекта. – Л.: Лениздат, 1963. –С. 130–135.
- Горовий В.І., Головенко В.П., Проценко О.О. та ін.Стресове нетримання сечі у жінок. – Вінобл-друкарня, 2003. – C. 44–47.
- Клебанов Б.М., Малетін І.І., Петко К.І., Шаваран С.С.,Ягупольський Л.М. Пат. України, №17071А опубл.18.03.1997. N-1?2?2? – триметилпропіл-N`-ціано-N``-арілгуанідини з фторвмісними замісниками в арильномуядрі, які проявляють гіпотензивні та кардіотонні дії.
- Лопаткин Н.А., Сивков А.В., Толстова С.С. “Импера-тивное недержание мочи” http://www.uro.ru/society/plenum/detrus.php3 – Сайт Рос. об-ва урологов.
- Лоран О.Б. Недержание мочи у женщин, этиология,патогенез, діагностика // http://www.uro.ru/society/plenum/detrus.php3 – Сайт Рос. об-ва урологов.
- Мохорт Н.А., Самарская И.В. Фармакодинамическаяактивность активаторов калиевіх каналов// Укр. мед.часопис. – 2003. – № 6(38). – С.26–37.
- Переверзев А.С. Недержание мочи у женщин//Здоров’я України. – 2001. – №7. – С. 9–12.
- Bonev A.D., Nelson M.T. ATP-sensitive potassium channelsin smooth muscle cells from guinea pig urinary bladder //Amer. J. Physiol. – 1993. – 264. – Р. C1190–C1200.
- 9. Buckner S.A., Milicic I., Daza A. Pharmacological andmolecular analysis of ATP-sensitive K+ channels in thepig and human detrusor// Eur. J. Pharmacol. – 2000. –400. – Р. 287–295.
- 10. Fry C.H. Experimental model to study the physiol-ogy, pathophysiology, and pharmacology of the lowerurinary tract// J. Pharmacol. Toxicol. Methods. – 2004. –49. – P.201–210.
- Gopalakrishnan M., Whiteaker K.L., Molinari E.J. et al.Characterization of the ATP-sensitive potassium channels(KATP) expressed in guinea pig bladder smooth muscle cells//J. Pharmacol. Exp. Ther. – 1999. – 289. – Р. 551–558.
- Hedlund H., Mattiasson A., Andersson K.E. Effects ofpinacidil on detrusor instability in men with bladder out-let obstruction// J. Urol. – 1991. – 146. –Р.1345–1347.
- Komersova K., Rogerson J.W., Conway E.L. et al. Theeffect of levcromakalim (BRL 38227) on bladder func-tion in patients with high spinal cord lesions// Brit. J.Clin. Pharmacol. – 1995. – 39. – Р. 207–209.
- Lawson K. Potassium channel activation: A potentialtherapeutic approach?// Pharmacol. Ther. – 1996. –70. – Р. 39–63.
- Nielsen-Kudsk J.E. Potassium channel modulation: anew drug principle for regulation of smooth muscle con-tractility// Dan. Med. Bull. – 1996. – 43. – Р. 429–447.
- Nurse D.E., Restorick J.M., Mundy A.R. The effect ofcromakalim on the normal and hyper-reflexic human detru-sor muscle// Brit. J. Urol. – 1991. – 68(Suppl 1). – Р.27–31.
- Petkov G.V., Heppner T.J., Bonev A.D. Low levels ofKATP channel activation decrease excitability and contrac-tility of urinary bladder // Amer. J. Physiol. Regul. Integr.Comp. Physiol. – 2001. – 280. – №5. – P.1427–R1433.
- Quayle J.M., Nelson M.T., Standen N.B. ATP-sensitiveand inwardly rectifying potassium channels in smoothmuscle// Physiol. Rev. – 1997. – 77. – Р.1165–1232.
- 19. Wammack R.U., Nawrath H., Hohenfellner R. Mechanicaland electrophysiological effects of cromakalim on the hu-mam urinary bladder// Eur.Urol. – 1994. – 26. – Р.176–181.
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