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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2005; 51(3): 57-66


Alterations in atp-dependence ofswelling- activated cl- currentassociated with neuroendocrinedifferentiation of lncap prostatecancer epithelial cells

R.M. Lazarenko, A.P. KondratskyN.Ch. Pogorela, Y.M. Shuba

    A.A.Bogomoletz Institute of Physiology, National Academy ofScience of Ukraine, Kyiv


Abstract

Increasing population of malignant, apoptosis resistant neuroendocrine (NE) cells due to differentiation of prostate epithelial/basal cells is a hallmark of advanced, androgen-inde- pendent prostate cancer, for which there is no successful therapy. Acquisition of apoptosis resistance involves alter- ations in the mechanisms of cell volume homeostasis, of which volume-regulate anion channels (VRAC) that carry swelling- activated Cl- current (ICl,swell) represent one of the key determi- nants. Given that VRAC function is generally known to be ATP-dependent, here we investigated how such dependence may evolve during NE differentiation of LNCaP prostate cancer epithelial cells. In the whole-cell patch-clamp recording mode ICl,swell could be activated in response to hypotonicity-induced cell swelling in control and NE-differentiated (by incubation in membrane-permeable cAMP analogs) LNCaP cells even fol- lowing total depletion of intracellular ATP using a cocktail of metabolic inhibitors. However, this basal ICl,swell had about 30% higher density and was less inactivating in NE-differenti- ated cells. Inclusion of 5 mM Mg-ATP in the patch pipette caused ICl,swell augmentation in both cell types. The augmenta- tion in the control cells was more prominent and occurred mostly at the expense of a non-inactivating current component. We conclude that ICl,swell in LNCaP cells consists of a non- inactivating, ATP-dependent and inactivating, ATP-indepen- dent components. NE differentiation promotes the increase of non-inactivating component and partial loss of its ATP sensi- tivity making the whole ICl,swell less ATP-sensitive as well. By largely avoiding the ATP metabolic control ICl,swell may contrib- ute to better control of cell volume under metabolic stress and thus enhance the survival rates of apoptosis-resistant NE cells.

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