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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2005; 51(2): 24-38


Central neuronal circuits of thebezold-jarish reflex in the rats

V.A. Maisky, A.I. Pilyavskii, A.V. Maznychenko,V.V. Datsenko, V.B. Pavlyuchenko,A.I. Kostyukov, А.А. Moibenko

    O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv


Abstract

The distribution of the Fos-immunoreactive (Fos-ir) and NADPH-diaphorase-positive/NOS-containing neurons in the medulla and thoracic spinal cord in rats was studied after the veratrine injection into v. jugularis ext. followed by reproduction of the Bezold-Jarisch reflex. Fos-ir neurons were found in the nucleus tractus solitarius (Sol), coudoventrolateral (CVL) and rostroventrolateral (RVL) areas, intermediate reticular nucleus (IRt), and lateral paragiganrocellular nucleus (LPGi) of the medulla. The intensity of c-fos expression was as follows: Sol > CVL > RVL > LPGi. The intensity of NADPH-diapho- rase reactivity was manifested in the following sequence: Sol > IRt > LPGi > RVL. In comparison to sham-operated animals, application of veratrine is associated with a statistically sig- nificant increase (P < 0.01) of the mean number of Fos-ir neurons in the Sol and CVL, but not in the RVL. Double- labelling (Fos + NADPH) was found in a small number (about 2%) of neurones in the Sol, IRt and LPGi. In the thoracic spinal cord Fos-ir neurons were predominantly detected in the superficial layers of the dorsal horn, area around the central canal and the intermediate lateral or medial nuclei (IMl and IMM), where NOS-containing neurons in the thoracic spinal cord were accumulated. Received data show that signals arising from the veratrine-activated cardiopulmonary afferents acti- vate neurons of the Sol and CVL that togеther with the RVL are incorporated into neuronal circuits for generation of the Bezold-Jarisch reflex. In the medulla NOS-containing neurons activated by input from cardiopulmonary receptors can con- tribute to the enhancement of cardioprotective depressor reflexes in the rats.

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