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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2005; 51(1): 88-94


Gastric secretion and mucosal bloodflow during simultaneous inhibitionof m1-cholinergic receptors andl-са[sup]2+[/sup]-channels in anaesthetized rats

A. Sklyarov Y. Mandryk

    Lviv National Medical University named after DanyloGalytsky


Abstract

The gastric secretion and gastric mucosal blood flow (GMBF) under simultaneous action of M1-cholinergic antagonist gastrocepine (3 mg/kg i.v.) and L-Са2+-channels blocker verapamile (1.25 mg/kg i.v.) were investigated in urethane- anaesthetized rats. The stomach was perfused with saline, and GMBF was measured by using hydrogen gas clearance tech- nique. The acid production under combined action of both blockers was lower by 57.3 % and 18.6 %, than in case of separate action of gastrocepine and verapamile accordingly. Pepsin concentration decreased by 32 %, which did not differ from verapamile action alone. The above changes of gastric secretion was accompanied by significant decrease of GMBF. Inhibition of M1-cholinergic receptors by gastrocepine did not show their key role in basal gastric secretion in rats, but they exacerbate L-Са2+-channels blocker action. We conclude, that enhance of acid output inhibition is related to the simulta- neous depression of nervous impulses in ganglionic neurons and acethylcholine release from parasympathetic postgangli- onic neurons. Synchronous block of M1-cholinergic receptors and L-Са2+-channels of smooth muscles stomach vessels resulted in reducing of gastric mucosal blood flow.

References

  1. Берегова Т.В. Роль блукаючих нервів у цент-ральному гальмуванні шлункової секреції: Авто-реф. … дис. д-ра біол.наук. – К., 2001. – 35 с.
  2. Гройсман С.Д., Губкин В.А., Береговая Т.В.Полуавтоматическая электрометрическая установкадля титрования желудочного сока //Лаб. дело. –1988. – №9. – С.66–67.
  3. Золотарев В.А., Ноздрачев А.Д. Капсаицинчувст-вительные эффекты блуждающего нерва // Рос.физиол. журн. им. И.М. Сеченова. – 2001. – 87, №2. –С.182–196.
  4. Al-Dohayan A.D. al-Tuwaijri A.S. The potential syn-ergic effect of calcium channel blockers and alpha-to-copherol on gastric mucosal injury induced by is-chaemia-reperfusion // Eur. J. Gastroenterol. andHepatol. – 1996. – 8, №11. – P.1107–1110.
  5. Ali A.T., Montgomery W.D., Santamore W.P., SpenceP.A. Preventing gastroepiploic artery spasm: papav-erine vs calcium channel blockade // J. Surg. Res. –1997. – 71, №1. – P.41–48.
  6. Del Tacca M., Danesi R., Blandizzi C., BernardiniM.C. A selective antimuscarinic agent: pirenzepine.Review of its pharmacologic and clinical properties //Minerva Dietol. Gastroenterol. – 1989. – 35, №3. –P.175–189.
  7. Glavin G.B. Verapamile and nifedipine effects on gas-tric acid secretion and ulcer formation in rats // J. Pharm.Pharmacol. – 1988. – 40, №7. – P.514–515.
  8. Herling A.W., Ljungstrom M. Effects of verapamil ongastric acid secretion in vitro and vivo // Eur. J.Pharmacol. – 1988. – 156, №3. – P.341–350.
  9. 9. Hulme E.C., Birdsall N.J.M, Buckley N.J. Muscarinicreceptor subtypes // Ann. Rev. Physiol. – 1990. – 30. –P.633–673.
  10. 10. Im W.B., Blakeman D.P., Mendlein J., Sachs G. Inhibi-tion of (H+, K+)-ATPase and H+ accumulation in hoggastric membranes by trifluoperazine, verapamil and8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate// Biochim. and Biophys. Acta. – 1984. – 770, №1. –P.65–72.
  11. Kajimura M., Reuben M.A., Sachs G. The muscarinicreceptor gene expressed in rabbit parietal cells is theM3 subtype // Gastroenterology. – 1992. – 23. P.1049–1056.
  12. Kato S., Okabe S., Takeuchi K. Pathways mediatingpentagastrin-induced mucosal blood flow response inrat stomachs // Dig. Diseases and Sci. – 1996. – 41,№3. – P.485–491.
  13. Ko J.K., Cho C.H., Ogle C.W. The vagus nerve and itsnon-cholinergic mechanism in the modulation of etha-nol-induced gastric mucosal damage in rats // J. Pharm.Pharmacol. – 1994. – 46, №1. – P.29–33.
  14. KojimaY., Ishihara K., Komuro Y. et al. Effects of themuscarinic receptor agonist carbachol and/or antago-nist pirenzepine on gastric mucus secretion in rats //Scand J. Gastroenterol. – 1993. – 28, №7. – P.647–651.
  15. Lindstrom E., Eliasson L., Bjorkqvist M., HakansonR. Gastric and neuropeptide PACAP evoke secretionfrom rat stomach histamine-containing (ECL) cells bystimulating influx through different Са2+ channels // J.Physiol. – 2001. – 535, №3. – P.663–677.
  16. Lio X.G., Cho C.H., Ko J.K. Felodipine- and ethanol-induced gastric mucosal damage in rats // Pharmacol-ogy. – 1995. – 51, №6. – P.391–397.
  17. Malcom P., Caulfield M.P., Birdsall N.J.M. Interna-tional Union of Pharmacology. XVII. Classification ofmuscarinic acetylcholine receptors // Pharmacol. Rev. –1998. – 50. – P.279–290.
  18. Murakami M., Moriga M., Miyake T., Uchino H.Contact Electrode Method in Hydrogen Gas Clearan-ceTechnique: A new Method for Determination ofRegional Gastric Mucosal Blood Flow in Animals andHumans // Gastroentorology. –1982. – 82, №3. –P.458–466.
  19. 19. Muto N. Changes in cytosolic free Ca2+ by histaminestimulation in isolated guinea-pig parieyal cells (sec-ond report) // Nippon Shokakibyo Gakkai Zasshi. –1989. – 86, №6. – P.1246–1253.
  20. 20. Oda M., Nakamura, Honda K. et al. Alterations in gas-tric mucosal microvascular endothelium in a stressedcondition-relevance to gastric ulcerogenesis // Adv. Exp.Med. Biol. – 1998. – 242. – P.161–175.
  21. Parkman H.P., Pagano A.P., Martin J.S., Ryan J.P. Elec-tric field stimulation-induced guinea pig gallbladdercontractions: role of calcium channels in acetylcholinerelease // Dig. Dis. Sci. – 1997. – 42. – P.1919–1925.
  22. Partosoedarso E.R., Blackshaw L.A. Roles of centralglutamate, acetylcholine and CGRP receptors in gas-trointestinal afferent inputs to vagal preganglionicneurones // Auton Neurosci. – 2000. – 83, №1–2. –P.37–48.
  23. Sandor A., Кidd M., Lawton G. et al. Neurohormonalmodulation of rat enterochromaffin-like cell histaminesecretion // Gastroenterology. – 1996. – 110. – P.1084–1092.
  24. Sigman H.H., Poleski M.H., Gillich A. Effect ofpirenzepine on acute mucosal erosions, gastric acidand mucosal blood flow in the spinal rat stomach //Digestion. – 1991. – 49, №4. – P.185–191.
  25. Sims S.M. Calcium and potassium currents in caninegastric smooth muscle cells // Amer. J. Physiol. – 1992. –262. – P.859–867.
  26. Tari A., Kuruhara Y., Yamanuchi R., Okahara S., SumiiK., Kajiyama G. Effects of omeprazole and pirenzepineon enterochromaffi-like cells and parietal cells in ratstomach // J. Gastroenterol. – 2001. – 36, № 6. –Р. 436–437.
  27. Urakawa T., Nagahata Y., Kawakita N. Effect ofpienzepine on postoperative gastric secretion // Kobe.J. Med. Sci. – 1993. – 39, №2. – P.69–79.
  28. Vanner S., Surprenant A. Neural reflexes controllingintestinal microcirculation // Amer. J. Physiol. – 1996. –271, №2, Pt 1. – P.223–230.
  29. 29. Yokotani K., DelValle J., Park J., Yamada T. MuscarinicM3 receptor-mediated release of gastrin from canineantral G cells in primary culture // Digestion. – 1995. –56, №1. – P.31–34.
  30. 30. Zeng N., Athmann C., Kang T. et al. Role ofneuropeptide – sensitive L-type Ca (2+) channels inhistamine release in gastric enterochromaffin-like cells// Amer. J. Physiol. – 1999. – 277, №6, Pt 1. – P.1268–1280.

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