T-[sup]786[/sup] c promoter allelicpolymorphism of endothelialno-synthase gene as genetic predictorof acute coronary syndrome
V.E.Dosenko, V.Yu.Zagoriy, Ya.M.Lutay, A.N.Parkhomenko, A.A.Moibenko
О.О. Bogomolets Institute of Physiology National Academyof Sciences of Ukraine, Kyiv
Abstract
Frequency of promoter endothelial NO-synthase gene allelic
polymorphism by using polymerase chain reaction and
restriction fragment length polymorphism (RFLP-PCR) was
determined in 221 patients with acute coronary syndrome
(ACS) and in 83 almost healthy subjects. Data obtained indi-
cate that different promoter allelic variant frequency differs
significantly in patients with ACS and in control group.
Correlation of normal homozygotes (Т/Т), heterozygotes
(Т/С) and pathologic homozysotes (С/С) was 48%, 36% and
16% respectively in patients, and in control it was 48%, 46%,
6% (Р<0.05 by c2-test). Thus, in patients with ACS in Ukrai-
nian population pathologic С/С variants of 5’- flanking region
of eNOS gene were found 2.7-times more often in ACS pa-
tients, than in control. This allows us to suggest, that this
allelic polymorphism can be considered as one of genetic risk
factors of ACS development.
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