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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2005; 51(1): 62-71


Тhapsigargin- sensitive and insensitiveintracellular са[sup]2+[/sup] stores in ratsubmandibular salivary gland acinarcells

О.V. Коpach, І.А. Kruglikov,N.V. Voitenko, N.V. Fedirko

    Ivan Franko National University of LvivA.A. Bogomolets Institute of Physiology National Academy ofSciences of Ukraine, Kyiv


Abstract

Acinar cells of rat submandibular salivary gland are characterized by heterogeneity of intracellular Ca2+ stores. In the present work we have studied this heterogeneity using Arsenazo III dye to measure a cellular total calcium content and Fura-2/AM, to determine free cytosolic calcium concentration ([Ca2+]i). We have found that the amount of Ca2+ released by inhibition of Ca2+ ATPase of the ER with thapsigargin comprises ~ 30 % of total ER calcium. This result was obtained in experiments on both intact and permeabilized acinar cells. We have also shown that both Ca2+ ATPase inhibi- tion with thapsigargin and emptying the stores with acetyl- choline (ACh) leaded to activation of store-operated Ca2+ influx (an increase in total calcium content of ~ 14 %). In permeabilized cells application of ACh after preincubation with thapsigargin leaded to a further decrease in total cellular calcium content (~ 38%). At the same time in intact cells it resulted in generation of [Ca2+]i transients with gradually de- creasing amplitudes. Thus, ACh is capable of producing an additional release of Ca2+ from thapsigargin-insensitive stores. This additional release is IP3 -dependent since it was completely blocked by heparin. We conclude that in acinar cells of rat submandibular gland thapsigargin-sensitive and thapsigargin- insensitive Ca2+ stores could exist.

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