Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2005; 51(1): 13-18


Attenuation of acetylcholine-inducedhyperpolarization of aorticendothelial cells in rats fedby high-salt diet and effect of enalapril

A.I. Bondarenko, V.F. Sagach

    О.О. Bogomolets Institute of Physiology National Academyof Sciences of Ukraine, Kyiv


Abstract

High-salt diet is known to result in attenuation of endothe- lium-dependent relaxation in rat aorta. We investigated the possible alterations in electrical responses of rat aortic endot- helial cells to acetylcholine as one of the eventual mechanisms that lead to inhibition of endothelium-dependent relaxation induced by high-salt diet. It was shown that endothelial resting membrane potential in rats fed by high-salt diet did not differ significantly from that observed in the control group (–40,6±2,3 mV and –41,2±0,9 mV, respectively). The amplitude of ace- tylcholine-induced hyperpolarization of endothelial cells in rats fed by high-salt diet was significantly attenuated (9,0±1,5 mV vs 22,7±1,3 mV in the control group). The amplitude of hyperpolarization increased to 25,7±1,3 mV when high-salt diet was administered with enalapril, the angiotensin-converting enzyme inhibitor. We conclude that high-salt diet leads to at- tenuation of hyperpolarization of rat aortic endothelial cells in response to acetylcholine, that may mediate inhibition of NO synthesis and endothelium-dependent relaxation. Angiotensin- converting enzyme inhibitor enalapril prevents the observed alterations in endothelial electrical responses induced by high- salt diet.

References

  1. Бондаренко О.І., Сагач В.Ф. Електричні реакціїендотелію аорти щурів із спонтанною гіпертензією//Фізіол. журн. – 2002. – 48, №4. – С. 75–79.
  2. Бондаренко О.І., Присяжна О.Д., Сагач В.Ф.Електричні реакції інтактного ендотелію аортищурів при експериментальному діабеті // Там само. –2004. – 50, №6. – С. 3–9.
  3. Яроцкий В.В., Ткаченко М.Н., Сагач В.Ф. Электри-ческие реакции эндотелия аорты крыс при действииацетилхолина и АТФ в условиях старения // Бюл.эксперим. биологии и медицины. – 2003. – 135, №3. –С. 257–260.
  4. Baluchnejadmojarad T., Roghani M., Imani A. Protec-tive effect of enalapril on vascular reactivity of the rataorta //Vasc. Pharmacol. – 2004. – 40. – Р.301–307.
  5. Berkenboom G., Brekine D., Unger P. et al. Chronicangiotensin-converting enzyme inhibition and endot-helial function in rat aorta // Hypertension. – 1995. –№26. – P.738–743.
  6. Bossaller C., Auch-Schwelk W., Weber F. et al. Endot-helium-dependent relaxations are augmented in ratschronically treated with the angiotensin – convertingenzyme inhibitor enalapril // J. Cardiovasc. Pharmacol. 1992. – 20 (suppl. 9). – S91–95.
  7. Clozel M., Kuhn H., Hefti F. Effects of angiotensinenzyme inhibitors and of hydralazine onendothelial function in hypertensive rats // Hyperten-sion. – 1990. – 16. – P.532–540.
  8. Creager M.A, Roddy M.A. Effect of captopril andenalapril on endothelial function in hypertensive pa-tients // Ibid. – 1994. – 24. – P.499–505.
  9. 9. Dohi Y., Criscione L., Pfeiffer K., Lьscher T.F. Angio-tensin blockade or calcium antagonists improve endot-helial dysfunction in hypertension: studies in perfusedmesenteric resistance arteries //J. Cardiovasc.Pharmacol. – 1994. – 24. – P.372–379.
  10. 10. Ferrario C.M., Richmond R.S., Smith R. et al. Renin-angiotensin system as a therapeutic target in managingatherosclerosis // Amer. J. Therap. – 2004. – 11(1). –P.44–53.
  11. Frisbee J.C., Sylvester F.A., Lombard J.H. High-saltdiet impairs hypoxia-induced cAMP production andhyperpolarization in rat skeletal muscle arteries // Ibid. –2001. – 281(4). – H1808–1815.
  12. Fujii K., Tominaga M., Ohmori S. et al. Decreased en-dothelium-dependent hyperpolarization to acetylcho-line in smooth muscle of the mesenteric artery ofsponstaneously-hypertensive rats // Circulat. Res. –1992. – 70. – P.660–669
  13. Fujii K., Ohmori S, Tominaga M. et al. Age-relatedchanges in endothelium-dependent hyperpolarizationin the rat mesenteric artery // Amer. J. Physiol. – 1993. –265. – H509–516.
  14. Fukao M., Hattori Y., Kanno M. et al. A. Alterationsin endothelium-dependent hyperpolarization andrelaxation in mesenteric arteries from stretozotocin-induced diabeic rats // Brit. J. Pharmacol. – 1997. –121. – P.1383–1391.
  15. Goto K., Fujii K., Onaka U., Abe I., Fujishima M.Renin-angiotensin system blockade improves endot-helial dysfunction in hypertension // Hypertension. –2000. – 36(4). – P.575–580.
  16. Goto K., Fujii K., Onaka U. et al. Angiotensin-converting enzyme inhibitor prevents age-related en-dothelium disfunction // Ibid. – 2000. – 36. – P.581–587.
  17. Hatta T., Nakata T., Harada S. et al. Lowering of bloodpressure improves endothelial dysfunction by increaseof nitric oxide production in hypertensive rats //Hypertens. Res. – 2002. – 25. – P.455–460.
  18. Hayakawa H., Coffee K., Raij L. Endothelial Dysfunc-tion and Cardiorenal Injury in Experimental Salt-Sen-sitive Hypertension. Effects of AntihypertensiveTherapy //Circulation. – 1997. – 96. – P.2407–2413.
  19. 19. Lenda D.M., Boegehold M.A. Effect of a high salt dieton oxidant enzyme activity in skeletal muscle micro-circulation // Amer. J. Physiol. – 2002. – 282. – H395–H402.
  20. 20. Lenda D.M., Sauls B.A., Boegehold M.A. Reactiveoxygen species may contribute to reduced endothe-lium-dependent dilation in rats fed high salt // Ibid. –2000. – 279. – H7–14.
  21. Lombard J.H., Sylvester F.A., Phillips S.A., FrisbeeJ.C. High-salt diet impairs vascular relaxation mecha-nisms in rats middle cerebral arteries // Ibid. – 2003. –284. – H1124–1133.
  22. McCarty M.F. Endothelial membrane potentialregulates production of both nitric oxide and superox-ide-a fundamental determinant of vascular health //Med. Hypotheses – 1999. – 53(4). – P.277–289.
  23. Mukai Y., Shimokawa H., Higashi M. et al. Inhibitionof renin-angiotensin system ameliorates endothelialdysfunction associated with aging in rats // Arterioscler.Thromb. Vasc. Biol. – 2002 . – 22(9). – P.1445–1450.
  24. Onaka U., Fujii K., Abe I., Fujishima M. Antihyper-tensive treatment improves endothelium-dependenthyperpolarization in the mesenteric artery of spon-taneously hypertensive rats // Circulation. – 1998. – 98. –P.175–182.
  25. Quaschning T., d’Uscio L.V., Shaw S., Luscher T.F.Vasopeptidase inhibition exhibits endothelial protec-tion in salt-induced hypertension // Hypertension. –2001. – 34. – P.943–1113.
  26. Shimoni Y. Inhibition of the formation or action ofangiotensin II reverses attenuated K+ currents in type1 and type 2 diabetes // J.Physiol. – 2001. – 537, №.1. –P. 83–92.
  27. Sohn H.-Y., Keller M., Gloe T. et al. The small G-protein Rac mediates depolarization-induced superox-ide formation in human endothelial cells // J. Biol.Chem. – 2000. – 275. – P.18745–18750.
  28. Sylvester F.A., Stepp D.W., Frisbee J.C., Lombard J.H.High salt diet depresses acetylcholine reactivity proxi-mal to NOS activation in cerebral arteries // Amer. J Physiol. – 2002. – 283. – H353–363.
  29. 29. Takase H., Moreau P., Kьng C.F. et al. Antihyperten-sive therapy prevents endothelial dysfunction inchronic nitric oxide deficiency: effect of verapamil andtrandolapril // Hypertension – 1996. – 27. – Р.25–31.
  30. 30. Van de Voorde J., Vanheel B., Leusen I. Endothelium-dependent relaxation and hyperpolarization in aortafrom control and renal hypertensive rats // Circulat.Res. 1992. – 70(1). – Р.1–8.
  31. Wojakowski W., Gminski J., Siemianowicz K. et al.The influence of angiotensin converting enzyme in-hibitors on lipid peroxidation in sera and aorta of rab-bits in diet-induced hypercholesterolemia // Int. J. Mol.Med. – 2000. – 6. – Р. 591–594.
  32. Zharikov S.I., Herrera H., Block E.R. Role of membranepotential in hypoxic inhibition of L-arginine uptakeby lung endothelial cells // Amer. J. Physiol. – 1997. –272. – L78–84.
  33. Zhu Z., Tepel M., Neusser M. et al. Effect of captoprilon vasoconstriction and Ca2+ fluxes in aortic smoothmuscle // Hypertension. – 1993. – 22(6). – Р.806–811.
  34. Zhu J., Mori T., Tianjian H., Lombard J.H. Effect ofhigh salt diet on NO release and superoxide produc-tion in rat aorta // Amer. J. Physiol. – 2004. – 286. –H575–583.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2018.