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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2005; 51(1): 13-18

Attenuation of acetylcholine-inducedhyperpolarization of aorticendothelial cells in rats fedby high-salt diet and effect of enalapril

A.I. Bondarenko, V.F. Sagach

    О.О. Bogomolets Institute of Physiology National Academyof Sciences of Ukraine, Kyiv



Abstract

High-salt diet is known to result in attenuation of endothe- lium-dependent relaxation in rat aorta. We investigated the possible alterations in electrical responses of rat aortic endot- helial cells to acetylcholine as one of the eventual mechanisms that lead to inhibition of endothelium-dependent relaxation induced by high-salt diet. It was shown that endothelial resting membrane potential in rats fed by high-salt diet did not differ significantly from that observed in the control group (–40,6±2,3 mV and –41,2±0,9 mV, respectively). The amplitude of ace- tylcholine-induced hyperpolarization of endothelial cells in rats fed by high-salt diet was significantly attenuated (9,0±1,5 mV vs 22,7±1,3 mV in the control group). The amplitude of hyperpolarization increased to 25,7±1,3 mV when high-salt diet was administered with enalapril, the angiotensin-converting enzyme inhibitor. We conclude that high-salt diet leads to at- tenuation of hyperpolarization of rat aortic endothelial cells in response to acetylcholine, that may mediate inhibition of NO synthesis and endothelium-dependent relaxation. Angiotensin- converting enzyme inhibitor enalapril prevents the observed alterations in endothelial electrical responses induced by high- salt diet.

Full text: (PDF, in Ukrainian)

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