Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(6): 118-127


Influenceof plant-originated gastroprotective and antiulcer substances on gastric mucosal repair

O.S. Zayachkivska, S.J. Konturek, D. Drozdowicz, T. Brzozowski, M.R. Gzhegotsky

    DepartmLviv National Medical University, Ukraine; Jagiellonian University College of Medicine, Cracow, Poland



Abstract

Fundamental basis of cellular and molecular mechanisms involved in mucosal injury and repair in gastrointestinal tract helps to develop new therapeutic approaches to various gut mucosal injury- related diseases. The study was aimed to as- sess the relations between plant-originated substances and their bioactivity measured in terms of antioxidant, cyto- protective and antiulceric activities and to determinate if these effects are capable of affecting the gastric mucosal lesions induced by absolute ethanol applied intragastrically. The fol- lowing plant-originated substances were considered: Solon, capsaicin, grapefruit-seed extract and amaranth. The area of gastric mucosa lesions and gastric blood flow were measured in rats with ethanol-induced lesions without (control) and with one of the tested substances without and with capsaicin dene- rvation of afferent nerves or administration of L-nitro-arginine (L-NNA), an inhibitor of nitric oxide synthase (NOS). Material/Methods: male Wistar rats, weighing 180-220 g fasted for 24 h before the study, 100% ethanol was applied ig to induced gastric lesions, whose area was determined by planimetry. Gastric blood flow was assessed using electro- lytic regional blood flowmeter. Results: All tested plant-originated substances afforded gastroprotection against ethanol-induced damage and this was accompanied by an increase in gastric microcirculation, both changes being reversed by pretreatment with neurotoxic dose of capsaicin or by pretreatment with L-NNA. Conclusions: Plant-originated substances are highly gastroprotective probably due to enhancement of the expres- sion of NOS I, NO release and an increase in gastric microcir- culation.

References

  1. Alarcon de la Lastra A.C., Martin M.J., MotilvaV. et al. Gastroprotection induced by silymarin, the hepatoprotective principle of Silybum marianum in ishemia-reperfusion mucosal injury: role of neutro- phils// Planta Med. – 1995. – 61 (20). – P.116–119.
  2. Bae E A , Han M J , Kim D H. In vitro anti-Helicobacter pylori activity of some flavonoids and their metabolites. – Planta Med. – 1999. – 65. – P. 441–443.
  3. Bagchi D. , Bagchi M. , Stohs S. et al. Cellular protec- tion with proanthocyanidins derived from grape seeds // Ann N. Y. Acad. Sci. – 2002. – 957. – P. 260–270.
  4. Bagchi D., Ray S.D., Bagchi M., Preuss H.G., Stohs S.J. Mechanistic pathways of antioxidant cyto- protection by a novel IH636 grape seed proantho- cyanidin extract // Indian J. Exp. Biol. – 2002.– 40(6). – P. 717–726.
  5. Bagchi D., Sen C.K., Ray S.D. et al. Molecular mecha- nisms of cardioprotection by novel grape seed proanthoceanidin extract // Mutat Res. – 2003. – 523– 524. – P. 87–97.92.
  6. Bandyopadhyay U., Biswas K., Chatterjee R. et al. Gastroprotective effects of Neem (Azadirachta indica) bark extract: possible involment of H(+)–K(+)–AT- Pase inhibition and scavenging of hydroxyl radical // Life Sci.– 2002.– 71(24).–P. 2845–2865.
  7. Bickel D., Roder T., Bestman H.J., Brune K. Identifi- cation and characterization of inhibitiors of peptido- leukotrienes syntesis from Petasites hybridus// Planta Med. – 1994. – 60(4). – P. 318–322.
  8. Blankson H., Grotterod E.M., Seglen P.O. Prevention of toxin-induced cytoskeletal disruption and apoptotic liver cell death by the grapefruit flavonoid, naringin // Cell Death Differ. – 2000. – 7. – P. 739–746.
  9. 9. Brzozowski T., Konturek P.C., Іliwowski Z. et al. Importance of nitric oxide and capsaicin-sensitive af- ferent nerves in healing of stress lesions induced by epidermal growth factor // J. Clin Gastroenterol. – 1997. – 25 (1). – P. 28–38.
  10. 10. Chao J.C., Hung H.C., Chen S.H., Fang C.L. Effects of Ginkgo biloba extract on cytoprotective factors in rats with duodenal ulcer // World J. Gastroenterol. – 2004. – 10(4). – P. 560–566.
  11. Dorababu M., Prabha T., Priyambada S., Agrawal V.K., Aryya N.C., Goel R.K. Effect of Bacopa monniera and Azadirachta indica on gastric ulceration and heal- ing in experimental NIDDM rats // Indian J. Exp. Biol. – 2004. – 42(4). – P.389–397.
  12. Farombi E.O., Alabi M.C., Akuru T.O. Kolaviron modulates cellular redox status and impairment of membrane protein activities induced by potassium bro- mate (KbrO(3)) in rats // Pharmacol Res. – 2002. – 45(1). – P. 63–68.
  13. Farombi E.O., Hansen M., Ravn-Haren G. et al. Commonly consumed and naturally occurring dietary substances affect biomarkers of oxidative stress and DNA damage in healthy rats // Food Chem. Toxicol. – 2004. – 42(8). – P. 1315–1322.
  14. Farombi E.O., Moller P., Dragsted L.O. Ex vivo and in vitro protective effects of kolaviron against oxygen derived radical induced DNA damage and oxidative stress in human lymphocytes and rat liver cells // Cell Biol Toxicol. – 2004. – 20(2). – P. 71–82.
  15. Fitzpatrick D.F., Bing B., Maggi D.A. et al. Vasodilating procyanidins derived from grape seeds // Ann N. Y. Acad. Sci. – 2002. – 957. – P. 78–89.
  16. Gastointestinal mucosal repair and experimental thera- peutics (Cho C.-H., Wang J.-Y.,eds). Frontiers of Gas- trointestinal Res – Basel. Karger – 2002, 25. – 251 p.
  17. Giamperi L., Fraternale D., Bucchini A., Rocco D. Antioxidant activity of Citrus paradisi seeds glyceric extract // Fitoterapia. – 2004. – 75(20). – P. 221–224.
  18. Havsteen B. Flavonoids with, a class of natural prod- ucts of high pharmacological potency // Biochem. Pharmacol. – 1983. – 32. – P. 1141–1149.
  19. 19. Heggers J.P., Cottingham J., Gusman J. et al. The effectiveness of processed grapefruit-seed extract as an antibacterial agent: II. Mechanism of action and in vitro toxicity // J. Altern Complement Med. – 2002. – 8. –P. 333–340.
  20. 20. Horoz L.M., Zubachyk W.M., Lukavetsky O.V. et al. Influence of thyroid gland hypofunction on apoptosis in oral mucous and salivatory glands// AML. – 2001. – 1. – P.70–78.
  21. Ito M., Suzuki Y., Ishihara M., Suzuki Y. Anti-ulcer effects of antioxidants: effect of probucol // Eur J. Pharmacol. – 1998. – 354(2–3). – P.189–196.
  22. Kim S.C., Byun S.H., Yang C.H. et al. Cytoprotective effects of Glycyrrhizae radix extract and its active component liquiritigenin against cadmium-induced tox- icity (effects on bad translocation and cytochrome c- mediated PARP cleavage)// Toxicology. – 2004. – 197(3). – P. 239–251.
  23. Konturek S.J., Brzozowski T., Drozdowicz D. et al. Gastroprotective and ulcer healing effects of solon, a synthetic flavonoid derivative of Sophoradin // Hepato- Gastroenterol.– 1987.– 34.– P.164–170.
  24. Konturek S.J., Kitler M.E., Brzozowski T., Radecki T. Gastric protection by meciadanol. A new synthetic flavonoid inhibiting histidine decarboxylase // Dig. Dis. Sci. – 1986. – 31. – P.847–852.
  25. Konturek S.J., Radecki T., Piastucki I. et al. Antiulcer and gastroprotective effect of Solon, a synthetic fla- vonoids derivative of sophoradin. Role of endogenous prostaglandins // Eur. J. Pharmacol. – 1986. – 21. – P. 6–10.
  26. Kyogoku K., Hatayma K., Yokomori S. et al. Anti- ulcer effect of isoprenyl flavonoids. Synthesis and anti-ulcer activity of a nea chalone related to sophoradin // Chem. Pharm. Bull. – 1979. – 27. – P. 2943–2952.
  27. Laurent C., Besancon P., Anger C. et al. Grape-seed extract affects proliferation and differentiation of 37 human intestinal Caco-2 cells // J. Agric. Food. Chem. – 2004. – 52(11). – P. 3301–3308.
  28. Liu C.F., Lin C.C., Lin M.H. et al. Cytoprotection by propolis ethanol extract of acute absolute ethanol-in- duced gastric mucosal lesions// Amer. J. Chin. Med. – 2002. – 30(2–3). – P. 35–254.
  29. 29. Llopiz N., Puiggros F., Cespedes E. et al. Antigenotoxic effects of grape seed procyanidin extract in Fao cells submitted to oxidative stress // J. Agric. Food. Chem. – 2004. – 52(5). – P. 1083–1087.
  30. 30. Moreno D.A., Ilic M., Poulev A. et al. Inhibitory ef- fects of grape seed extract on lipases. – Nutrition. – 2003. – 19(10). – P. 876–879.
  31. Motilva V., Alarcon de la Lastra C., Martin M.J. Ul- cer-protecting effects of naringenin on gastric lesions induced by ethanol in rat: role of endogenous pros- taglandins // J. Pharm. Pharmacol. – 1994. – 46. – P. 91–94.
  32. Natella F., Belelli F., Gentili V., Ursini F., Scaccini C. Grape seed proanthocyanidins prevent plasma post- prandial oxidative stress in humans // J. Agric. Food. Chem. – 2002. – 50(26). – P. 7720–7725.
  33. Navarrete A., Trejo-Miranda J.L., Reyaes-Trjo L. Prin- ciples of root bark of Hippocratea excelasa (Hippocra- taceae) with gastroprotective activity// J. Ethnophar- macol. – 2002. – 79(3). – P. 383–388.
  34. Pastrada-Bonilla E., Akoh C.C., Sellappan S., Krewer G. Phenolic content and antioxidant capacity of muscadine grapes // J. Agric. Food. Chem. – 2003. – 51(18). –P. 5497–5503.
  35. Preuss H.G, Bagchi D., Bagchi M. Protective effects of a novel niacin-bound chromium complex and a grape seed proanthocyanidin extract on advancing age and various aspects of syndrome X.-Ann N. Y. Acad. Sci. – 2002. – 957. – P. 250–259.
  36. Raji Y., Ogunwande I.A., Osadebe C.A., John G. Ef- fects of Azadirachta indica extract on gastric ulcer- ation and acid secretion in rats // J. Ethnopharmacol. – 2004. – 90(1). – P. 167–170.
  37. Rang H.P., Dale M.M., Ritter J.M. Pharmacology. – Churchill Livingstone, 1995. – P. 615.
  38. Rao V.S., Santos F.A., Solreira T.T., Souza M.F., Melo C.L. et al. Investigations on the gastroprotective and antidiarrhocal properties of ternatin, a tetrametho- xyflavone from Egletes viscose //Planta Med. – 1997. – 63 (20). – P. 146–149.
  39. 39. Reagor L., Gusman J., McCoy L., Carino E., Heggers J.P. The effectiveness of processed grapefruit-seed extract as an antibacterial agent: I. An in vitro agar assay // J. Altern. Complement Med. – 2002. – 8. – P. 325–332.
  40. 40. Reyes M., Martin C., Alarcon de la Lastra C., Trujillo J., Toro M.V. et al. Antiulcerogenicity of the flavonoid fraction from Erica andevalensis Cabezudo-Rivera// Z. Naturforsch. – 1996. – 51 (7–8). – P. 563–569.
  41. Saeki T/, Ohno T/, Boku K/ et al. Mechanism of pre- vention by capsaicin of ethanol–induced gastric mu- cosal injury – a study in the rat using intravital micros- copy // Aliment Pharmacol Ther. – 2000. –143. – P. 135–144.
  42. Sano A., Ymakoshi J., Tokutake S., Tobe K. et al. Pro- cyanidin B1 is detected in human serum after intake of proanthocyanidin-rich grape seed extract // Biosci Biotechnol Biochem. – 2003. – 67(5). – P. 1140–1143.
  43. Shirwaikar A., Bhilegaonkar P.M., Malini S., Kumar J.S. The gastroprotection activity of the ethanol ex- tract of Ageratum conyzoides // J. Ethonopharmacol. – 2003. – 86(1). – P.117–121.
  44. So F.V., Guthrie N., Chambers A.F., Moussa M., Carroll K.K. Inhibition of human breast cancer cell proliferation and delay of mammary tumorogenesis by flavonoids and citrus juices // Nutr. Cancer. – 1996. – 26. – P.167–181.
  45. Szocsanyi J., Bartho L. Capsaicin-sensitive afferents and their role in gastroprotection: an update. – J. Physiol. Pharmacol. – 2001. – 95. – P. 181–188.
  46. Tsybina T., Dunaevsky Y., Musolyamov A., Egorov T. et al. New protease inhibitors from buckwheat seeds: properties, partial aminoacid sequences and possible biological role // Biol. Chem. – 2004. – 385(5). – P. 429–434.
  47. Uchiyama T., Kamikawa H., Ogita Z., Yakugaku Zasshi. Anti-ulcer effect of extract from phellodendri cortex// Eur J. Pharmacol. – 1989. – 109(9). – P. 672–676.
  48. Yimaz Y., Toledo R.T. Major flavonoids in grape seeds and skins: antioxidant capacity of catechin, epicatechin, and gallic acid // J. Agric. Food. Chem. – 2004. – 52(2). – P. 255–260.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.