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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(6): 62-75

Effect of muscle pain on c-fos expression and nadph-diaphorase activityin the spinal cord

A.V. Maznychenko

    A.A. Bogomoletz Institute of Physiology, National Academy ofSciences of Ukraine, Kiev.



Abstract

Capsaicin (Sigma, 5 mg/1 ml) was unilaterally injected into dorsal neck muscles (mm. trapezius, splenius) of the cat (n = 6) anesthetized with ?-chloralose. The laminar distribution of Fos- immunoreactive (Fos-ir) and NADPH-d-reactive neurons was studied in the sections of the cervical and lumbar seg- ments. In comparison to sham operated animals (n = 2), 2 h after capsaicin injection the significant c-fos expression was found throughout the cervical cord (C1 – C8) ipsilaterally and lumbar cord bilaterally (L1 – L7), while the predominant mean number of Fos-ir neurons was detected in the C1 – C4 and L3 – L6 segments. In cervical spinal cord, c-fos expression was three times more than in lumbar spinal cord. Dorsal neck nox- ious stimulation produced widespread c-fos expression in the large and small labeled cells within lamina I, at the neck of the dorsal horn (lamina V) and also in the medial part of the inter- mediate zone (lamina VII) where cells of origin of the long crossed and uncrossed descending propriospinal pathways to the lumbar cord were known to be localized. The main accu- mulations of NADPH-d-reactive cells were registered within the marginal zone (laminae I and II) and medial part of the intermediate zone (lamina VII). Fos-ir neurons were co-dis- tributed with NADPH-d-reactive cells within the marginal zone, and the neck of the dorsal horn, however, double-stain- ing spinal neurons were not registered. These results strongly suggest that the revealed c-fos expression in cervical and lumbar segments depends on the activity of vanilloid receptors of the cervical muscles and confirmed the anatomical coupling of both regions through long propriospinal pathways which may be underlying in the cervico-lumbar nociceptive processing. An influence of the spino-cerebro-spinal loop on modulation c-fos expression in these regions is not excluded also.

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