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Possible role of ammonium in paracrine regulations of uterus functions
IU. V. Danylovych
Palladin Institute of Biochemistry, National Academy ofSciences of Ukraine, Kyiv.
Abstract
An opportunity of formation of ammonia (NH3) in utera en-
dometrium and its influence on exchange of Са2+ and Н+ in
plasmalemma of myometrium was investigated. Dissociation
of endometrium stroma cells and myocytes suspension was
carried from utera of pigs and rats in accordance with the
traditional techniques. In suspension of stroma cells a rather
high АМР-deaminase activity (53±2 mmol IМР/hour on 1 mg
of protein) was determined. It was demonstrated that ammo-
nia release in extracellular space (measured by the changes of
colouring of trinitrobenzolsulfonate acid) was significantly
amplified by 1 mM acetylcholine and decreased by 0,1 mM
fluoride ions, nonspecific АМР-deaminase inhibitor. It en-
ables to assume a role of АМР-deaminase in formation of NH3
by endometrium stroma cells and its release into extracellular
space during acetylcholine stimulation. The addition of am-
monia (4mМ) to suspension of myocytes is accompanied by
significant increase in рН (measured by the change in BCECF
fluorescence) in extracellular and intracellular space, and the
last parameter is inhibited by the blockers of passive Н+ trans-
port across the membrane: 0,1 mM 4-aminopyridine and tet-
raethylammonium. It is possible that addition of ammonia-
containing solution results in increase in proton gradient on
myocyte membrane and in amplification of Н+ efflux. The
opportunity of stimulation of acetylcholine-activated passive
Са2+ transport in myocytes by 4 mM NH4+ that was sup-
pressed by 1 mM cadmium and 1 nМ nifedipine was also
shown using fluorescent probe FURA-2AM. The increase in
Са2+concentration in cytoplasm in the given conditions is in-
tensively oppressed by protonophore (0,04 % 2,4-dinitro-
phenol) and is effectively amplified by Na+/Н+-exchange in-
hibitor 0,1 mM amyloride. It is possible to assume an
amplification of lygand-activated passive Са2+ transport caused
by dispersion of transmembrane proton gradient which exists
on plasmalemma and can be increased by ammonia formation
in endometrium. The role of diffused from endometrium NH3 in
regulation of utera functional activity requires further
investigation, however already at this stage it is possible to
assume, that NH3 molecules (or ion NH4+) can carry out a role of
paracrine regulator in the system endometrium-myometrium.
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