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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(6): 48-55


Possible role of ammonium in paracrine regulations of uterus functions

IU. V. Danylovych

    Palladin Institute of Biochemistry, National Academy ofSciences of Ukraine, Kyiv.


Abstract

An opportunity of formation of ammonia (NH3) in utera en- dometrium and its influence on exchange of Са2+ and Н+ in plasmalemma of myometrium was investigated. Dissociation of endometrium stroma cells and myocytes suspension was carried from utera of pigs and rats in accordance with the traditional techniques. In suspension of stroma cells a rather high АМР-deaminase activity (53±2 mmol IМР/hour on 1 mg of protein) was determined. It was demonstrated that ammo- nia release in extracellular space (measured by the changes of colouring of trinitrobenzolsulfonate acid) was significantly amplified by 1 mM acetylcholine and decreased by 0,1 mM fluoride ions, nonspecific АМР-deaminase inhibitor. It en- ables to assume a role of АМР-deaminase in formation of NH3 by endometrium stroma cells and its release into extracellular space during acetylcholine stimulation. The addition of am- monia (4mМ) to suspension of myocytes is accompanied by significant increase in рН (measured by the change in BCECF fluorescence) in extracellular and intracellular space, and the last parameter is inhibited by the blockers of passive Н+ trans- port across the membrane: 0,1 mM 4-aminopyridine and tet- raethylammonium. It is possible that addition of ammonia- containing solution results in increase in proton gradient on myocyte membrane and in amplification of Н+ efflux. The opportunity of stimulation of acetylcholine-activated passive Са2+ transport in myocytes by 4 mM NH4+ that was sup- pressed by 1 mM cadmium and 1 nМ nifedipine was also shown using fluorescent probe FURA-2AM. The increase in Са2+concentration in cytoplasm in the given conditions is in- tensively oppressed by protonophore (0,04 % 2,4-dinitro- phenol) and is effectively amplified by Na+/Н+-exchange in- hibitor 0,1 mM amyloride. It is possible to assume an amplification of lygand-activated passive Са2+ transport caused by dispersion of transmembrane proton gradient which exists on plasmalemma and can be increased by ammonia formation in endometrium. The role of diffused from endometrium NH3 in regulation of utera functional activity requires further investigation, however already at this stage it is possible to assume, that NH3 molecules (or ion NH4+) can carry out a role of paracrine regulator in the system endometrium-myometrium.

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