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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(6): 27-31

Role of nitric oxide in the corpuscavernosum reactions evoked by hydrogen peroxide

A.V.Gurkovska, K.Yu.Sukhanova, V.A.Bouryi, V.F.Sagach

    A.A.Bogomoletz Institute of Physiology, National Academy ofScienses of the Ukraine, KievInstitute of Applied Problems of Physics and Biophysics,National Academy of Sciences of the Ukraine, Kiev.



Abstract

Norpus cavernosum smooth muscle spends the majority of its time in the contracted state consisting of tonic and tetanic components. Tetanic component is a result of superposition of phasic contrations ocuring spontaneously with a frequency of 5 - 27 /minute. Hydrogen peroxide (H2O2) in concentration of 10-3 mol/l causes a transient increase in tetanic contraction lasting 5 to 8 minutes, followed by either recovery of spontaneous activity or inhibition of its frequency and muscle strip relaxation. 30 minutes pretreatment with the specific blocker of NO syntase, L-NAME, does not affect the intrinsic spontaneous activity parameters but enhances H2O2 evoked reaction, increasing both the amplitude and duration of the transient contraction by 41,2 ± 14,5 and 52,5 % ± 22,8 % (n=9) correspondingly. By contrast, the exogenous NO donors nitroglycerin and sodium nitroprusside, cause spontaneous activity inhibition and muscle strip relaxation resulting in the decrease of I2I2 evoked contraction and duration by 8,7 ± 3,7 and 24,5 % ± 10,6 % (n=7) correspondingly. These results suggest that tetanic contraction is an important component of corpus cavernosum smooth muscle tone which is modulated by free radical oxygen species and controlled by NO dependent mechanism.

Full text: (PDF, in Ukrainian)

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