Electrical responses of intact aortic endothelialcells in streptozotocin-induced diabetic rats
O.I. Bondarenko, O.D. Prysyazhna, V.F. Sagach
Bogomoletz Institute of Physiology National Academy ofSciences of Ukraine.
![](/images/cite.png)
Abstract
The changes in electrical properties of intact aortic endothelial
cells from streptozotocin-induced diabetic rats were
investigated. The mean resting membrane potential of
unstimulated endothelium was significantly less negative
(-32.7±0.8 mV) as compared with the aged-matched control
group (-41.2 ±0.9 iA). In diabetic rat aortic strips, acetylcholine
(2 µM) hyperpolarized endothelial cells to -57.6±1.1 mV,
whereas in control group the hyperpolarization reached -
64.4±0.9 mV. The amplitude of the hyperpolarization in diabetic
rats, however, was not significantly different from the
control group (24.9±1.1 mV and 23.2±0.9 mV, respectively).
It was concluded that endothelial cells of diabetic rat aorta
have less negative membrane potential values at rest and during
acetylcholine stimulation that may partially account for
the suppressed endothelium-dependent relaxation in
streptozotocin-induced diabetic rats.
References
- Бондаренко О.І., Сагач В.Ф. Електричні реакції ендотелію аорти щурів із спонтанною гіпертензією //Фізіол. журн. – 2002. – 48, №4. С. 75–79.
- Присяжная А.Д., Ткаченко М.Н., Коцюруба А.В., Сагач В.Ф. Нарушение NO-зависимого механизма сосудистой реактивности при экспериментальном сахарном диабете. – В кн.: Труды III международной научно-практической конференции «Дисфункция эндотелия: экспериментальные и клинические исследования». 18–20 мая 2004 г. – Витебск, 2004 г. – С. 113–116.
- Сагач В.Ф., Ткаченко М.М., Присяжна О.Д. та ін. Зміни вазодилататорних реакцій судинних гладеньких м’язів та системи оксиду азоту за умов експери- ментального цукрового діабету// Фізіол. журн. – 2003. – 49, №4. – С. 24–32.
- De Vriese A., Verbeuren T., Van de Voorde J., Lameire N., Vanhoutte P. Endothelial dysfunction in diabetes // Brit. J.Pharmacol. – 2000. – 130. – Р. 963– 974.
- Hattori Y., Kawasaki H., Abe K., Kanno M. Superox- ide dismutase recovers altered endothelium-relaxation in diabetic rat aorta // Amer. J. Physiol. – 1991. – 261. – P. H1086–H1094.
- Fukao M., Hattori Y., Kanno M. et al. Alterations in endothelium-dependent hyperpolarization and rela- xation in mesenteric arteries from stretozotocin-in- duced diabeic rats // Brit. J. Pharmacol. – 1997. – 121. – P. 1383–1391.
- Kamata K, Miyata N, Kasuya Y. Functional changes in potassium channels in aortas from rats with strep- tozotocin-induced diabetes // Eur. J. Pharmacol. – 1989. – 18. – №166. – P. 319–323.
- Kamata K, Miyata N, Kasuya Y. Impairment of endot- helium-dependent relaxation and changes in levels of cyclic GMP in aorta from streptozotocin-induced diabe- tic rats // Brit. J. Pharmacol. – 1989. – 97. – P. 614–618.
- 9. Luckhoff A., Busse R. Calcium influx into endothelial cells and formation of endothelium-derived relaxing factor is controlled by the membrane potential // Pflug. Arch. – 1990. – 416. – P. 305–311.
- 10. Nilius, B., Droogmans, G. Ion channels and their func- tional role in vascular endothelium // Physiol. Rev. – 2001. – 81. – P. 415–459.
- Oyama Y., Kawasaki H., Hattori Y., Kanno M. At- tenuation of endothelium-dependent relaxation in aorta from diabetic rats // Eur. J. Pharmacol. – 1986. – 132. – P.75–78.
- Pieper G.M. Enhanced, unaltered and impaired nitric oxide-mediated endothelium-dependent relaxation in experimental diabetes mellitus: importance of disease duration // Diabetologia. – 1999. – 42. – P.204–213.
- Shimizu K., Muramatsu M., Kakegawa Y., Asano H., Toki Y., Miyazaki Y., Okumura K., Hashimoto H., Ito T. Role of prostaglandin H2 as an endothelial-derived contracting factor in diabetic state // Diabetes. – 1993. – Електричні реакції інтактного ендотелію 42. – P. 1246–1252.
- Takeda Y., Miyamori I., Yoneda T. Takeda R., Produc- tion of endothelin-1 from the mesenteric arteries of streptozotocin-induced diabetic rats // Life Sci. – 1991. – 48. – P. 2553–2556.
- Tare M., Coleman H.A., Parkington H.C. Regional dif- ferences in the regulation of vascular smooth muscle relaxation by the endothelium in health and in diabetes // Neurophysiology. – 2003. – 35(3– 4). – P. 372.
- Wigg S.J., Tare M., Tonta, M.A. et al. Comparison of effects of diabetes mellitus on an EDHF-dependent and EDHF-independent artery // Amer. J. Physiol. – 2001. – 281. – P. H232–H240.
- Zharikov S.I., Herrera H., Block E.R. Role of membrane potential in hypoxic inhibition of L-arginine uptake by lung endothelial cells // Amer. J. Physiol. – 1997. – 272, №1, Pt 1. – P. L78–84.
|