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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(6): 3-8


Electrical responses of intact aortic endothelialcells in streptozotocin-induced diabetic rats

O.I. Bondarenko, O.D. Prysyazhna, V.F. Sagach

    Bogomoletz Institute of Physiology National Academy ofSciences of Ukraine.


Abstract

The changes in electrical properties of intact aortic endothelial cells from streptozotocin-induced diabetic rats were investigated. The mean resting membrane potential of unstimulated endothelium was significantly less negative (-32.7±0.8 mV) as compared with the aged-matched control group (-41.2 ±0.9 iA). In diabetic rat aortic strips, acetylcholine (2 µM) hyperpolarized endothelial cells to -57.6±1.1 mV, whereas in control group the hyperpolarization reached - 64.4±0.9 mV. The amplitude of the hyperpolarization in diabetic rats, however, was not significantly different from the control group (24.9±1.1 mV and 23.2±0.9 mV, respectively). It was concluded that endothelial cells of diabetic rat aorta have less negative membrane potential values at rest and during acetylcholine stimulation that may partially account for the suppressed endothelium-dependent relaxation in streptozotocin-induced diabetic rats.

References

  1. Бондаренко О.І., Сагач В.Ф. Електричні реакції ендотелію аорти щурів із спонтанною гіпертензією //Фізіол. журн. – 2002. – 48, №4. С. 75–79.
  2. Присяжная А.Д., Ткаченко М.Н., Коцюруба А.В., Сагач В.Ф. Нарушение NO-зависимого механизма сосудистой реактивности при экспериментальном сахарном диабете. – В кн.: Труды III международной научно-практической конференции «Дисфункция эндотелия: экспериментальные и клинические исследования». 18–20 мая 2004 г. – Витебск, 2004 г. – С. 113–116.
  3. Сагач В.Ф., Ткаченко М.М., Присяжна О.Д. та ін. Зміни вазодилататорних реакцій судинних гладеньких м’язів та системи оксиду азоту за умов експери- ментального цукрового діабету// Фізіол. журн. – 2003. – 49, №4. – С. 24–32.
  4. De Vriese A., Verbeuren T., Van de Voorde J., Lameire N., Vanhoutte P. Endothelial dysfunction in diabetes // Brit. J.Pharmacol. – 2000. – 130. – Р. 963– 974.
  5. Hattori Y., Kawasaki H., Abe K., Kanno M. Superox- ide dismutase recovers altered endothelium-relaxation in diabetic rat aorta // Amer. J. Physiol. – 1991. – 261. – P. H1086–H1094.
  6. Fukao M., Hattori Y., Kanno M. et al. Alterations in endothelium-dependent hyperpolarization and rela- xation in mesenteric arteries from stretozotocin-in- duced diabeic rats // Brit. J. Pharmacol. – 1997. – 121. – P. 1383–1391.
  7. Kamata K, Miyata N, Kasuya Y. Functional changes in potassium channels in aortas from rats with strep- tozotocin-induced diabetes // Eur. J. Pharmacol. – 1989. – 18. – №166. – P. 319–323.
  8. Kamata K, Miyata N, Kasuya Y. Impairment of endot- helium-dependent relaxation and changes in levels of cyclic GMP in aorta from streptozotocin-induced diabe- tic rats // Brit. J. Pharmacol. – 1989. – 97. – P. 614–618.
  9. 9. Luckhoff A., Busse R. Calcium influx into endothelial cells and formation of endothelium-derived relaxing factor is controlled by the membrane potential // Pflug. Arch. – 1990. – 416. – P. 305–311.
  10. 10. Nilius, B., Droogmans, G. Ion channels and their func- tional role in vascular endothelium // Physiol. Rev. – 2001. – 81. – P. 415–459.
  11. Oyama Y., Kawasaki H., Hattori Y., Kanno M. At- tenuation of endothelium-dependent relaxation in aorta from diabetic rats // Eur. J. Pharmacol. – 1986. – 132. – P.75–78.
  12. Pieper G.M. Enhanced, unaltered and impaired nitric oxide-mediated endothelium-dependent relaxation in experimental diabetes mellitus: importance of disease duration // Diabetologia. – 1999. – 42. – P.204–213.
  13. Shimizu K., Muramatsu M., Kakegawa Y., Asano H., Toki Y., Miyazaki Y., Okumura K., Hashimoto H., Ito T. Role of prostaglandin H2 as an endothelial-derived contracting factor in diabetic state // Diabetes. – 1993. – Електричні реакції інтактного ендотелію 42. – P. 1246–1252.
  14. Takeda Y., Miyamori I., Yoneda T. Takeda R., Produc- tion of endothelin-1 from the mesenteric arteries of streptozotocin-induced diabetic rats // Life Sci. – 1991. – 48. – P. 2553–2556.
  15. Tare M., Coleman H.A., Parkington H.C. Regional dif- ferences in the regulation of vascular smooth muscle relaxation by the endothelium in health and in diabetes // Neurophysiology. – 2003. – 35(3– 4). – P. 372.
  16. Wigg S.J., Tare M., Tonta, M.A. et al. Comparison of effects of diabetes mellitus on an EDHF-dependent and EDHF-independent artery // Amer. J. Physiol. – 2001. – 281. – P. H232–H240.
  17. Zharikov S.I., Herrera H., Block E.R. Role of membrane potential in hypoxic inhibition of L-arginine uptake by lung endothelial cells // Amer. J. Physiol. – 1997. – 272, №1, Pt 1. – P. L78–84.

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