Electrical responses of intact aortic endothelialcells in streptozotocin-induced diabetic rats
O.I. Bondarenko, O.D. Prysyazhna, V.F. Sagach
Bogomoletz Institute of Physiology National Academy ofSciences of Ukraine.
Abstract
The changes in electrical properties of intact aortic endothelial
cells from streptozotocin-induced diabetic rats were
investigated. The mean resting membrane potential of
unstimulated endothelium was significantly less negative
(-32.7±0.8 mV) as compared with the aged-matched control
group (-41.2 ±0.9 iA). In diabetic rat aortic strips, acetylcholine
(2 µM) hyperpolarized endothelial cells to -57.6±1.1 mV,
whereas in control group the hyperpolarization reached -
64.4±0.9 mV. The amplitude of the hyperpolarization in diabetic
rats, however, was not significantly different from the
control group (24.9±1.1 mV and 23.2±0.9 mV, respectively).
It was concluded that endothelial cells of diabetic rat aorta
have less negative membrane potential values at rest and during
acetylcholine stimulation that may partially account for
the suppressed endothelium-dependent relaxation in
streptozotocin-induced diabetic rats.
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