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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(5): 29-37


Sensitivity ofphenylarsineoxide-induced mitochondrial permeability transition pore opening in old ratheart and under condition of intermittent hypoxic training

Rudyk I.V., Vavilova G.L., Strutynska N.A. Kotsuruba A.V., Sagach V.F.

    A.A.Bogomoletz Institute of Physiology, National Academy ofScience of Ukraine, Kiev


Abstract

We studied the sensitivity of mitochondrial permeability tran- sition pore (MPTP) opening to its inductor – phenylarsineoxide (PAO) in adult (6 months), old rat heart (24 months) and in old rat heart under conditions of intermittent hypoxic training (IHT). We defined the sensitivity of MPTP opening on two parameters: the alterations in mitochondrial swelling and the release of mitochondrial substances (mitochondrial factor). It was shown that mitochondria of old rat heart are more sensi- tive to PAO which caused opening of cyclosporin-sensitive MPTP and MPTP-dependent factor release, in comparison with those of adult rat heart mitochondria. One of the causes of increased sensitivity of MPTP opening to PAO is develop- ment of an oxidative stress with age that was accompanied by increase of an active metabolite of oxygen: hydrogen peroxide (Н2О2) and hydroxyl radical (.ОН) in old rat heart mitochon- dria. It was observed corrective effect of IHT on PAO-induced MPTP-opening and MPTP-dependent factor release from old rat heart mitochondria. IHT also reduced the content of Н2О2 and .ОН in old rat hearts. IHT can be used as protective procedure preventing MPTP opening in aging and, probably, in a numerous chronic pathological states of organism under oxidative stress.

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