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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(5): 3-10


The role of the mitochondrial permeabilitytransition pore in the development of a dog.s skeletal muscle fatigue

Boguslavskiy A.Y., Dmitrieva A.V., Sagach V.F.

    A.A.Bogomoletz Institute of Physiology, National Academy ofScience of Ukraine, Kiev


Abstract

On anaesthetized dogs the role of the mitochondrial permeability transition .ira (m.O.) in the development of the m. gastrocnemius fatigue was investigated. In a control series of experiments it was shown, that 10 short-term (30'.) electrical stimulation (8 Hz, 5 ms, 20 V) with 5" interval resulted in significant reduction of the muscle contractions force (more than 40%) and considerably increased oxygen cost of a m.gastercnemicus work (more than 130%), comparison with initial parameters. The registered inhibition of the muscle contraction force pointed on the development of m. gastrocnemius fatigue, that was accompanied by an appearance in blood from v. femoralis mitochondrial factor (MF), which, as shown by us earlier, is a marker of the mPTP opening. Preliminary injection of selective inhibitor of the mPTP opening cyclosporine A (0,012 mg/kg, i.v.) resulted in the pronounced fall of the MF concentration in blood from v. femoralis, in comparison with the parameters registered under control conditions. Pretreatment with the another inhibitor of the mPTP opening melatonin (0,75 mg/kg, i.v.) prevented inhibition of the muscle contractions force and reduction of the efficiency of oxygen using by m.gastercnemicus under conditions similar control. Thus, m.gastercnemius fatigue didn.t develop, and the MF concentration in blood from v. femoralis was much lower, than in the control, that testified to absence of the mPTP opening. So then, fatigue development was accompanied by inhibition of the muscle contractions force, marked reduction of the efficiency of oxygen using by m.gastercnemicus that was prevented by the mPTP opening inhibitors. Thus, the mPTP opening can underlie the development of the working skeletal muscle fatigue.

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