The specific pattern of c-fos gene expression was evaluated within the lumbo-sacral spinal cord during 30 min non-noxious stimulation n. gastrocnemius-soleus of male Wistar rats. A lot of small and large Fos-immunoreactive (Fos-ir) nuclei were located ipsilaterally, mainly in the middle of the neck and base of the dorsal horn (layers 5 and 6). Traditionally, these layers are considered to be the region of termination of the low-threshold afferent fibers. A few Fos-ir neurons were recorded in the intermediate zone (layer 7) and the area around central canal (layer 10). The scarce labeled cells were also revealed within the marginal zone and substantia gelatinosa of the dorsal horn, i. e. the zones of termination of the highthreshold muscle afferents. In conclusion, the revealed pattern of c-fos expression in the spinal cord following non-noxious stimulation differs from that induced by noxious stimulation. Thus, the pattern of c-fos expression in the spinal cord is dependent on the stimulus modality.