Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(4): 108-116


Expression of c-fos in the spinalcord after electrical stimulation of the low-threshold afferent fibers n. gastrocnemius-soleusof the rat

A.V. Maznychenko, V.A. Maisky, A.I. Pilyavskii, A.I. Kostyukov

    A.A. Bogomoletz Institute of Physiology, National Academy ofSciences of Ukraine, Kiev.


Abstract

The specific pattern of c-fos gene expression was evaluated within the lumbo-sacral spinal cord during 30 min non-noxious stimulation n. gastrocnemius-soleus of male Wistar rats. A lot of small and large Fos-immunoreactive (Fos-ir) nuclei were located ipsilaterally, mainly in the middle of the neck and base of the dorsal horn (layers 5 and 6). Traditionally, these layers are considered to be the region of termination of the low-threshold afferent fibers. A few Fos-ir neurons were recorded in the intermediate zone (layer 7) and the area around central canal (layer 10). The scarce labeled cells were also revealed within the marginal zone and substantia gelatinosa of the dorsal horn, i. e. the zones of termination of the highthreshold muscle afferents. In conclusion, the revealed pattern of c-fos expression in the spinal cord following non-noxious stimulation differs from that induced by noxious stimulation. Thus, the pattern of c-fos expression in the spinal cord is dependent on the stimulus modality.

References

  1. Костюк П.Г. Двухнейронная рефлекторная дуга. – М.: Медгиз, 1959. – 256 с.
  2. Abercrombie M. Estimation of nuclear population from microtome section // Anat. Res. – 1946. – 94. – P. 239–247.
  3. Brown A.G. Organization of the Spinal Cord. The Anatomy and Physiology of Identified Neurons. – New York: Springer, 1981. – 238 p.
  4. Buritova J., Honore P., Chapman V., Besson J.-M. Carrageenan oedema and spinal Fos-LI neurons are re- duced by piroxicam in the rat // Neuroreport. – 1995. – 6. – P. 1257–1262.
  5. Eccles J.C., Kostyuk P.G., Schmidt R.F. Central path- ways responsible for depolarization of primary afferentfibres // J. Physiol. (Lond.) – 1962. – 161. – P. 237–257.
  6. Eccles J.C., Kostyuk P.G., Schmidt R.F. The effect of electric polarization of the spinal cord on central affer- ent fibres and on their excitatory synaptic action // Ibid. – 162. – P. 138–150.
  7. Herdegen T., Rudiger S., Mayer B. et al. Expression of nitric oxide syntase and colocalization with Jun, Fos and Krox transcription factors in spinal cord neurons following noxious stimulation of the rat hindpaw // Brain. Res. Mol. Brain. Res. – 1994. – 22. – P. 245–258.
  8. Hsu S.-M., Raine L., Fanger H. Use of avidin-biotin- peroxidase complex (ABC) in immunoperoxidase tech- niques: a comparison between ABC and unlabelled anti- body (PAP) procedures // J. Histochem. Cytochem. – 1981. – 29. – P. 577–580.
  9. 9. Jasmin L., Gogas K.R., Ahlgren S.C. et al. Walking evokes a distinctive pattern of Fos-like immunoreac- tivity in the caudal brainstem and spinal cord of the rat // Neurosci. – 1994. – 58, №2. – P. 27–286.
  10. 10. Kalueff A.V., Maisky V.A., Pilyavskii A.I., Makarchuk N.E. Persistent c-fos expression and NADPH-d reactivity in the medulla and the lumbar spinal cord in rat with short-term peripheral anosmia // Neurosci. Lett. – 2001. – 301. – P. 13–134.
  11. Lima D., Avelino A. Spinal expression is differentially induced by brief and persistent noxious stimulation // Neuroreport. – 1994. – 5. – P. 1853–1856. 12. McLaughlin B.J., Barber R., Saito K. et al. Immuno-cytochemical localization of glutamate decarboxylase in rat spinal cord // J. Comp. Neurol. – 1975. – 164. – P. 305–322.
  12. Menetrey D., Gannon A., Levine J.D., Basbaum A.I. Expression of c-fos protein in interneurons and pro- jection neurons of the rat spinal cord in response to noxious somatic, articular and visceral stimulation // Ibid. – 1989. – 285. – P. 177–195.
  13. Mense S., Craig A.D., Jr. Spinal and supraspinal ter- minations of primary afferent fibers from the gastroc- nemius-soleus muscle in the cat // Neurosci. – 1988. – 26, №3. – P. 1023–1035.
  14. Molander C., Hongpaisan J., Grant G. Changing pat- tern of c-fos expression in spinal cord neurons after electrical stimulation of the chronically injured sciatic nerve in the rat // Ibid. – 1992. – 50, №1. – P. 223–236.
  15. Paxinos G., Watson C. The Rat Brain in Stereotaxic Coordinates. – San Diego: Acad. Press. – 1997.
  16. Pilyavskii A.I., Maisky V.A., Kalezic I. et al. c-fosexpression and NADPH-diaphorase reactivity in spi- nal neurons after fatiguing stimulation of hindlimb mus- cles in the rat // Brain. Res. – 2001. – 923. – P. 91–102.
  17. Todd A.J., Spice R.C., Brodbelt A.R. et al. Some in- hibitory neurons in spinal cord develop c-fos immunoreactivity after noxious stimulation // Neurosci. – 1994. – 63. – P. 805–816.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2018.