Developmental changes in the distribution ofcorticosteroid-binding globulin in fetal and newborn sheep tissues
A. G. Reznikov, J. R. G. Challis, E. T. M. Berdusco
MRC Group in Fetal and Neonatal Health and Development,Lawson Research Institute, and Departments of Physiologyand Obstetrics and Gynecology, University of WesternOntario,St. Joseph, s Health Centre, London, Ontario N6A 4V2,Canada.
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Abstract
Developmental changes in immunohistochemical localization
of corticosteroid-binding globulin (CBG) in fetal and newborn
sheep tissues were studied. Tissue samples have been harvested
at days 63.64, 100.103, 125.128 and 142.144 of gestation
or 2 postnatal days. In the liver, immunoreactive CBG (irCBG)
has been identified in hepatocytes. The intensity of CBG
staining was highest at 63.64th gestation days and then was
lowered gradually down to negligible level in newborn lambs.
Within kidney, irCBG was selectively localized to the epithelium
of proximal and distal convoluted tubules. Its amount in
the course of development followed a pattern similar to that in
the liver. By contrast, fetal sheep lung and pancreas demonstrated
noticeable rise of irCBG late in gestation. irCBG has
been detected in respiratory epithelium of tertiary bronchi,
bronchioles and terminal bronchioles, meanwhile alveoli and
other lung tissues were CBG-immunonegative. In the pancreas,
irCBG staining was associated with acinary cells,
whereas Langerhans islets contained no irCBG at all examined
stages of pregnancy. Developmental changes in irCBG did not
follow reported triphasic profile of fetal sheep plasma CBG
concentrations thereby showing the existence of independent
cellular mechanisms regulating CBG level in the tissues. Peculiarities
of intraorgan distribution and developmental changes
in irCBG suggest that intracellular CBG may regulate
bioavailable cortisol concentrations in the sheep tissues during
fetal and early postnatal life.
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