Developmental changes in the distribution ofcorticosteroid-binding globulin in fetal and newborn sheep tissues
A. G. Reznikov, J. R. G. Challis, E. T. M. Berdusco
MRC Group in Fetal and Neonatal Health and Development,Lawson Research Institute, and Departments of Physiologyand Obstetrics and Gynecology, University of WesternOntario,St. Joseph, s Health Centre, London, Ontario N6A 4V2,Canada.
Developmental changes in immunohistochemical localization
of corticosteroid-binding globulin (CBG) in fetal and newborn
sheep tissues were studied. Tissue samples have been harvested
at days 63.64, 100.103, 125.128 and 142.144 of gestation
or 2 postnatal days. In the liver, immunoreactive CBG (irCBG)
has been identified in hepatocytes. The intensity of CBG
staining was highest at 63.64th gestation days and then was
lowered gradually down to negligible level in newborn lambs.
Within kidney, irCBG was selectively localized to the epithelium
of proximal and distal convoluted tubules. Its amount in
the course of development followed a pattern similar to that in
the liver. By contrast, fetal sheep lung and pancreas demonstrated
noticeable rise of irCBG late in gestation. irCBG has
been detected in respiratory epithelium of tertiary bronchi,
bronchioles and terminal bronchioles, meanwhile alveoli and
other lung tissues were CBG-immunonegative. In the pancreas,
irCBG staining was associated with acinary cells,
whereas Langerhans islets contained no irCBG at all examined
stages of pregnancy. Developmental changes in irCBG did not
follow reported triphasic profile of fetal sheep plasma CBG
concentrations thereby showing the existence of independent
cellular mechanisms regulating CBG level in the tissues. Peculiarities
of intraorgan distribution and developmental changes
in irCBG suggest that intracellular CBG may regulate
bioavailable cortisol concentrations in the sheep tissues during
fetal and early postnatal life.
- Ali S., Basset J.R., Jones M.R., Wynn P.C. The devel- opment of corticosteroid binding globulin-like activity in fetal sheep blood // J. Develop. Physiol. – 1992. – 18 – P. 13–18.
- Ballard P.L., Kitterman J.A., Bland R.D. et al. Ontog- eny and regulation of corticosteroid binding globulin capacity in plasma of fetal and newborn lambs // Endo- crinology. – 1982. – 110. – P. 359–366.
- Berdusco E.T.M., Hammond G.L., Jacobs R.A. et al. Glucocorticoid-induced increase in plasma cortico- steroid-binding globulin levels in fetal sheep is associ- ated with increased biosynthesis and alterations in glycosylation // Endocrinology. – 1993. – 132. – P. 2001–2008.
- Berdusco E.T.M., Milne W.K., Challis J.R.G. Low- dose cortisol infusion increases plasma corticosteroid- binding globulin (CBG) and the amount of hepatic CBG mRNA in fetal sheep on day 100 of gestation // J. Endocrinol. – 1994. – 140. – P. 425–430.
- Berdusco E.T.M., Yang K., Hammond G.L., Challis J.R.G. Corticosteroid-binding globulin (CBG) produc- tion by hepatic and extra-hepatic sites in the ovine fetus; effects of CBG on glucocorticoid negative feed- back on pituitary cells in vitro // J. Endocrinol. – 1995. – 146. – P. 121–130.
- Challis J.R.G., Berdusco E.T., Jeffray T.M. et al. Corticosteroid-binding globulin (CBG) in fetal deve- lopment // J. Steroid Biochem. Molec. Biol. – 1995. – 53. – P. 523–527.
- Dobrovic-Jenik D., Milkovic S. Regulation of fetal Na+/ K+-ATPase in rat kidney by corticosteroids // Biochim. Biophys. Acta. – 1988. – 942. – P. 227–235.
- Funder J.W., Pearce P.T., Smith R., Ian Smith A.Mineralocorticoid action: Target tissue specificity is enzime, not receptor, mediated // Science. – 1988. – 242. – P. 583–585.
- 9. Hammond G.L. Molecular properties of corticosteroid binding globulin and the sex-steroid binding proteins / / Endocr. Rev. – 1990. – 11. – P. 65–79.
- 10. Hammond G.L., Smith C.L., Goping I.S. et al. Primary structure of human corticosteroid binding globulin, deduced from hepatic and pulmonary cDNAs, exhibits homology with serine protease inhibitors // Proc. Natl. Acad. Sci. USA. – 1987. – 84. – P. 5153–5157.
- Hammond G.L., Smith C.L., Underhill D.A. Molecu- lar studies of corticosteroid binding globulin structure, biosynthesis and function // J. Steroid Biochem. Molec. Biol. – 1991. – 40. – P. 755–762.
- Heo J., Kattesh H.G., Roberts M.P., Schneider J.F. Plasma levels of cortisol and corticosteroid-binding globulin and hepatic CBG mRNA expression in pre- and postnatal pigs // Domest. Animal Endocrinol. – 2003. – 25. – P. 263–273.
- Hill K.J., Lumbers E.R., Elbourne I. The actions of cortisol on fetal renal function // J. Develop. Physiol. – 1988. – 10. – P. 85–96.
- Hsu B.R.-S., Siiteri P.K., Kuhn R.W. Interactions be- tween corticosteroid-binding globulin (CBG) and tar- get tissues / Binding proteins of steroid hormones. Forest M.G., Pugeaut M. (eds). London: John Libbey Eurotext, 1986. – P. 577–591.
- Langlois D.A., Matthews S.G., Yang K. Differential expression of the 11b-hydroxysteroid dehydrogenase 1 and 2 in the developing ovine fetal liver and kidney / / J. Endocrinol. – 1995. – 147. – P. 405–411.
- Liggins G.C., Fairclough R.J., Crieves S.A. et al. The mechanism of initiation of parturition in the ewe // Rec. Progr. Horm. Res. – 1973. – 29. – P. 111–150.
- Rall L., Pictet R., Githens S., Rutter W.J. Glucocorti- coids modulate the in vitro development of the embryonic rat pancreas // J. Cell Biol. – 1977. – 75. – P. 398–409.
- Rosner W. The functions of corticosteroid-binding globulin and sex hormone-binding globulin: recent ad- vances // Endocrind. Rev. – 1990. – 11. – P. 80–91.
- 19. Scrocchi L.A., Orava M., Smith C.L. et al. Spatial and temporal distribution of corticosteroid-binding globu- lin and its messenger ribonucleic acid in embryonic and fetal mice // Endocrinology. – 1993a. – 132. – P. 903–909.
- 20. Scrocchi L.A., Hearn S.A., Han V.K.M., Hammond G.L. Corticosteroid-binding globulin biosynthesis in the mouse liver and kidney during postnatal development // Ibid. – 1993b. – 132. – P. 910–916.
- Seralini G.-E., Smith C.L., Hammond G.L. Rabbit corticosteroid-binding globulin: primary structure and biosynthesis during pregnancy // Molec. Endocrinol. – 1990. – 4. – P. 1166–1172.
- Siiteri P.K., Murai J.T., Hammond G.L. et al. The se- rum transport of steroid hormones // Rec. Progr. Horm. Res. – 1982. – 38. – P. 457–510.
- Sloboda D.M., Newnham J.P., Challis J.R. Repeated maternal glucocorticoid administration and the devel- oping liver in fetal sheep // J. Endocrinol. – 2002. – 175. – P. 535–543.
- Thorburn G.D., Challis J.R.G. Endocrine control of parturition // Physiol. Rev. – 1979. – 59. – P. 863–918.
- Wintour E.M., Coghlan J.P., Towstoless M. Cortisolis natriuretic in the immature ovine fetus // J. Endocrinol. – 1985. – 106. - R13–R15.
- Yang K., Smith C.L., Dales D., Hammond G.L., Challis J.R.G. Cloning of an ovine 11в-hydroxysteroid dehy- drogenase complementary deoxyribonucleic acid; tis- sue and temporal distribution of its messenger ribonucleic acid during fetal and neonatal development // Endocrinology. – 1992. – 131. – P. 2120–2126.