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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(4): 79-84


Functional nicotinic acetylcholine receptors inthe neurons of rat intracardiac ganglia

H.E. Purnyn, O.V. Rikhalsky, M.V. Skok, V.I. Skok

    A.A.Bogomoletz Institute of Physiology National Academy ofSciences, Kiev;Palladin Institute of Biochemistry National Academy ofSciences, Kiev.


Abstract

The alpha subunit composition of nicotinic acetylcholine receptors (nAChRs) expressed in the neurons of intracardiac ganglia of the rat was investigated using monoclonal and polyclonal antibodies against a3, a4, a5 and a7 nAChR subunits. The acetylcholine-induced membrane potentials in the neurons of the subepicardial plexus isolated from the left atrium of the heart were studied by intracellular recording performed in the presence of subunit-specific nAChR-blocking antibodies applied either separately or in various combinations. It was found that intracardiac ganglia neurons express a3a5; a7; a7(a5) and a4-containing nAChRs. The neurons were heterogeneous as to the nAChR subtypes expressed that possibly indicated their functional differences.

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