Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(4): 73-78


A-subunit composition of neuronal nicotinic acetylcholinereceptors in the submucosal plexus of the guinea-pig

O.O.Grigorov, M.V.Skok, V.I.Skok

    A.A.Bogomoletz Institute of Physiology National Academy ofSciences of Ukraine, Kiev.


Abstract

The a-subunit composition of nicotinic acetylcholine receptors (nAChRs) in the submucosal plexus of the guinea-pig ileum were studied using the whole-cell patch-clamp technique and affinity-purified antibodies (Abs) against a3-, a4, a5 and a7- subunits of nAChRs. The independent addition of anti-a3 and anti-a5 Abs to extracellular bath solution induced depression of the acetylcholine (ACh)-evoked currents by 60%±1,56% and 65%±1,62% correspondingly. Successive application of anti-a5 Abs in the presence of anti-a3 Abs did not have any additional blocking effect on ACh-evoked currents. Anti-a7 Abs evoked depression of Ach-induced currents by 24%±1,51% in 80% of investigated neurons and by 67%±1,5% in 20% of neurons. The addition of anti-a4 Abs to extracellular bath solution did not have effect on membrane currents of the investigated neurons. Our data provide evidence of participation of a3-, a5 and a7-containing receptors in the response to ACh. a3- and a5-subunits are associated in the same functional nAChRs that provide greater part of response to ACh in the most of submucosal neurons. However, in some neurones a7-containing nAChRs provide greater part of response to agonist.

References

  1. Коваль О.М., Скок М.В., Скок В.І. а-субодиничний склад нікотинових холінорецепторів нейронів нижнього ближового ганглія морської свинки: електрофізіологічне дослідження // Нейрофизио- логия/Neurophysiology. – 2003. – 35, № 1. – С. 9–19.
  2. Costa M., Furness J.B., Gibbins I.L. Chemical coding in en- teric neurons // Prog. Brain Res. – 1986. – 68. – Р. 217–239.
  3. Evans R.J., Jiang M.M., Surprenant A. Morphological properties and projections of electrophysiologically characterized neurons in the guinea-pig submucosal plexus // Neuroscience – 1994. – 59. – Р. 1093–1110.
  4. Galligan J.J., LePard K.J., Schneider D.A., Zhou X. Multiple mechanisms of fast excitatory synaptic transmission in the enteric nervous system // JАNS. – 2000. – 81. – Р. 97–103.
  5. Glushakov A.V., Glushakova H.Y., Skok V.I. Modula- tion of nicotinic acetylcholine receptor activity in submucous neurons by intracellular messengers // JANS. – 1999. – 75. – Р. 16–22.
  6. Glushakov A.V., Voitenko L.P., Skok M.V., Skok V.I. Distribution of neuronal nicotinic acetylcholine receptors containing different alpha-subunits in the submucosal plexus of the guinea-pig // Auton Neurosci. – 2004. – 110, №1. – Р. 19–26.
  7. Hamill O.P., Marty A., Neher E. et al. Improved patch- clamp techniques for high resolution current recording from cell and cell-free membrane patches // Pflugers Arch. – 1981. – 391. – Р. 85–100.
  8. Kirchgessner A.L., Liu M.T. Immunohistochemical localization of nicotinic acethylcholine receptors in the guinea-pig bowel and pancreas // J.Comp. Neurol. – 1998. – 390. – Р. 497–514.
  9. 9. Lindstrom J. Neuronal nicotinic acetylcholine recep- tors. – In: Narahashi T. (ed.). Ion Channels. New York: Plenum pres. – 1996. – 4. – Р. 377–390.
  10. 10. Lindstrom J. Nicotinic acetylcholine receptors in health and disease // Molec. Neurobiol. – 1997. – 15, №2. – Р. 193–222.
  11. Lukas Q.J., Changeux J.P., Le Novere N. et al. International Union of Pharmacology XX. Current status of the nomenclature for nicotinic acetylcholine receptors and their subunits // Pharmacol. Rev. – 1999. – 51, №2. – Р. 397–401.
  12. Obaid A.L., Koyano T., Lindstrom J. et al. Spatio- temporal patterns of activity in an intact mammalian network with single-cell resolution: optical studies of nicotinic activity in an enteric plexus // J. Neurosci. – 1999. –19. – Р. 3073–3093.
  13. Purnyn E., Skok M., Rikhalsky O., Skok V. Subunit composition of nicotinic acetylcholine receptors in neurons of the rat intracardiac ganglia // Нейрофи- зиология/Neurophysiology. – 2002. – 35, № 1. – Р. 203–206.
  14. Ramirez-Latorre J., Yu C.R., Qu X. et al. Functional contribution of alpha 5 subunit to neuronal acetyl- choline receptor channels // Nature. – 1996. – 380. – Р. 347–351.
  15. Salamone F., Zhou M. Aberrations in nicotinic acetyl- choline receptor structure, function, and expression: implications in disease // McGill J. of Med. – 2000. – 5, №2. – Р. 90–97.
  16. Sha L., Love A., Ma R.C., Szurszewski J.C. Cholin- ergic transmissionin pancreatic ganglia of the cat // Pan- creas. – 1997. – 14. – Р. 83–93.
  17. Skok M.V., Voitenko L.P., Voitenko S.V. et al. Alpha subunit composition of nicotinic acetylcholine receptors in the rat autonomic ganglia neurones as de- termined with subunit-specific anti-a(181–192) pep- tide antibodies // Neuroscience – 1999. – 93. – Р. 1427–1436.
  18. Skok M.V., Lykhmus E., Bobrovnik S. et al. Structure of epitopes recognized by the antibodies to a(181– 192) peptides of neuronal nicotinic acetylcholine receptors: extrapolation to the structure of acetyl- choline-binding domaine // J. Neuroimmunol. – 2001. – 121. – Р. 59–66.
  19. 19. Zhou X., Galligan J.J. Nicotinic cholinergic receptors in cultured myenteric neurons of guinea-pig ileum // Soc. Neurosci. Abs. – 1996. – 22. – Р. 335.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2018.