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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(4): 50-56


Activation ofryanodine receptors influences the pair-pulsed depression in cultured rat hippocampalneurons

M.A. Kravchenko, A.O. Moskalyuk, Y.O. Kolodin, N.S. Veselovsky, S.A. Fedulova

    International Center for Molecular Physiology, Kiev;A.A. Bogomoletz Institute of Physiology National Academy ofSciences of Ukraine, Kiev.


Abstract

Role of intraterminal calcium stores in modulation of shortterm plasticity of evoked inhibitory postsynaptic currents (IPSCs) was studied in synaptically connected cultured hippocampal neurons using patch-clamp technique in whole-cell configuration. Currents were induced by voltage stimulation which were applied externally to presynaptic fiber. Paired stimuli resulted in paired-pulse depression (n=18) or facilitation (n=7) of the second IPSC at interpulse intervals 150 and 500 ms. Calcium release from intracellular calcium stores was activated by local application of caffeine and ryanodine, ryanodine receptor agonists. One of the characteristics of shortterm plasticity, the pair-pulsed ratio (ratio of amplitudes of second IPSC to first IPSC), decreased during addition of ryanodine (50 nM) from 0.79±0.02 to 0.71±0.04 (n=10). This change was observed only for cells that demonstrated pairpulsed depression. We also studied the influence of caffeine and ryanodine on spontaneous currents. Attenuation of the mean amplitude to 0.71±0,06 and frequency to 0.42±0.08 (n=7) of spontaneous IPSCs was observed during application of caffeine (10 mM). Upon ryanodine application the mean amplitude did not change but frequency of spontaneous events decreased to 0.74±0.09 (n=12). The amplitudes of currents evoked by fast local application of g-aminobutyric acid (GABA 100 mM) were diminished in the presence of caffeine (10 mM) to 0.59±0.03 (n=5) and in the presence of ryanodine (50 nM) to 0.56±0.11 (n=7). Thus we conclude that endoplasmic reticular calcium stores are able to modulate synaptic transmission from both presynaptic (in assumption that short term plasticity and spontaneous activity are believed to have presynaptic nature) and postsynaptic (since GABA-receptors are situated on postsynaptic cell) sides.

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