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Estimating transmitter release rates and quantal amplitudes in central synapsesfrom postsynaptic current fluctuations
A.R. Stepanyuk, Y.A. Boychuk, T.N. Tsugorka, Y.I. Drebot, I.V. Lushnikova, T.A. Pivneva,P.V. Belan
A. A. Bogomoletz Institute of Physiology, NationalAcademy of Sciences of Ukraine, Kiev.
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Abstract
Several approaches recently introduced to analyze release rates
in central synapses advanced our understanding of synaptic
neurotransmission, however, leaving many questions still unresolved.
In this work we present evidence that a new method
recently developed by Sakaba and Neher to study neurotransmission
in calyx of Held, a giant glutamatergic synapse,
could be also applied for estimating release rate functions and
averaged quantal sizes in small central synapses. By means of
different simulation approaches applied to reproduce
GABAergic neurotransmission in the hippocampus we have
shown that possible problems with a spatial voltage clamp
which can occur in synaptic connections distributed over a
large area of dendritic tree are not crucial for applicability of
the method when synapses are compactly distributed or located
proximally and when release rates are below 1 ms-1. In another
set of simulations we have also shown that at above mentioned
release rates desensitization and/or saturation of postsynaptic
GABAA receptors does not prevent accurate estimates of
release rate and averaged quantal size. Thus, we conclude that
the new approach based on analysis of fluctuations of postsynaptic
currents under conditions of stationary release or moderately
nonstationary conditions might be applicable to studies
of small central synapses.
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© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.
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