Spontaneous neuronal activity in rathippocampal cell cultures: effect of glucose deprivation
S.Y. Ivanova, M.V. Storozhuk, E.P. Kostyuk, P.G. Kostyuk
International Center of Molecular Physiology,A.A. Bogomopletz Institute, National Academy of Sciences ofUkraine, Kiev
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Abstract
Spontaneous neuronal activity was studied in rat hippocampal
cell cultures using patch clamp technique in cell-attached
or loose-patch configuration. It was found that in spite of
relatively low average frequency (1-2 Hz) of neuronal activity
in the cell cultures, neurons often fire doublets or triplets of
action potentials with interspike interval of 60 ms and less.
Interspike interval histograms were substantially better fitted
by double exponential decay functions than by single exponential
ones. On average, estimated decay time constant for
the fits were t1 ~ 36, ms and t2 ~ 1000 ms respectively. Spontaneous
neuronal firing to a large extent depended on
glutamatergic excitation and GABAAergic inhibition: a blocker
of AMPA/kainate receptor CNQX (10 mM) either substantially
decreased or completely blocked spontaneous action
potentials; a blocker of GABAA receptors bicuculine (10 mM)
increased neuronal firing. Effect of glucose deprivation on action
potential frequency was also studied. It was found that
glucose deprivation reduces AP frequency to 25% of control.
Taken together, these results support an idea that hypoglycaemia
alters synaptic transmission in hippocampus.
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