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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(4): 16-21


Spontaneous neuronal activity in rathippocampal cell cultures: effect of glucose deprivation

S.Y. Ivanova, M.V. Storozhuk, E.P. Kostyuk, P.G. Kostyuk

    International Center of Molecular Physiology,A.A. Bogomopletz Institute, National Academy of Sciences ofUkraine, Kiev


Abstract

Spontaneous neuronal activity was studied in rat hippocampal cell cultures using patch clamp technique in cell-attached or loose-patch configuration. It was found that in spite of relatively low average frequency (1-2 Hz) of neuronal activity in the cell cultures, neurons often fire doublets or triplets of action potentials with interspike interval of 60 ms and less. Interspike interval histograms were substantially better fitted by double exponential decay functions than by single exponential ones. On average, estimated decay time constant for the fits were t1 ~ 36, ms and t2 ~ 1000 ms respectively. Spontaneous neuronal firing to a large extent depended on glutamatergic excitation and GABAAergic inhibition: a blocker of AMPA/kainate receptor CNQX (10 mM) either substantially decreased or completely blocked spontaneous action potentials; a blocker of GABAA receptors bicuculine (10 mM) increased neuronal firing. Effect of glucose deprivation on action potential frequency was also studied. It was found that glucose deprivation reduces AP frequency to 25% of control. Taken together, these results support an idea that hypoglycaemia alters synaptic transmission in hippocampus.

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