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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(4): 10-15

Chronic treatment with gabaa receptor antagonist bicuculline increasesefficacy of gabaergic synaptic transmission in rat hippocampal cell cultures

S.Y. Ivanova, P.G.Kostyuk

    International Center of Molecular Physiology, NationalAcademy of Sciences of Ukraine, Kiev.



Abstract

It has been proposed to consider plasticity in neuronal net- work as occurring in two forms: use-dependent plasticity which modifies the network properties, and homeostatic plasticity which may counteract use-dependent changes. Сhronic block of some of transmitter receptors is often used as a model for studying homeostatic plasticity. We studied whether chronic block of GABAA receptors can affect GABAergic transmis- sion. Using whole-cell voltage clamp recording and local extra- cellular stimulation we investigated evoked inhibitory postsyn- aptic currents (IPSCs) in cultured rat hippocampal neurons grown in the presence of GABAA receptor antagonist – bicuculline (20мM) and in control conditions. Cell for both control and pretreated cultures were obtained from same dis- section and were grown in parallel. We compared the ampli- tudes of the evoked IPSC, the reversal potentials of the responses IPSC coefficient of variation and depression evoked by paired stimulation. Chronic bicuculline treatment did not significantly affect the paired-pulse depression (PPD) and IPSC reversal potentials. In contrast we found that amplitude of evoked IPSCs was increased about two times in cultures treated with bicuculline. However, IPSC coefficients of varia- tion were not significantly different. We conclude that chronic block of GABAA receptors enhances efficacy of GABAergic synaptic transmission in rat hippocampal cell cultures and this effect is likely to postsynaptic mechanism(s) because IPSC increase was not accompanied with changes of IPSC coefficient of variation. A possibility that the effect of chronic block of GABAA receptors on GABAergic transmission is mediated by neurotrophin BDNF is discussed.

Full text: (PDF, in Ukrainian)

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