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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(3): 16-23

Regulation of intracellular calciumreleasedchannels in secretory cells of chironomus plumosus larvae by cyclicnucleotides

S.V.Bychkova, V.V. Man’ko, M.Yu.Klevets

    I.Franko Univercity, Lviv



Abstract

We investigate action of cAMP and cGMP on calcium con- tent in tissue of salivary gland of Chironomus plumosus larvae L. using arsenaso III. In order to permeabilize cells we incu- bate it in medium with saponine (0,1 mg/ml during 10 min) with next incubation in necessary solution. The internal incu- bation solution contents (in mM): 15.3 NaCl, 129.94 KCl, 0.35 Na2HPO4, 0.44 KH2PO4, 5.55 glucose, pH 7.0. It was shown that cAMP (1mkM) didn’t cause release calcium through IP3-channels, because IP3 didn’t intensify affect of cAMP, which has been established before. We observed that cAMP (100mkM) intensified ryanodine’s action to increase calcium content in saponin-treated gland tissue. We concluded that cAMP (100mkM) activated calcium releasing though ry- anodine-sensitive channels and inhibited IP3-dependent calcium releasing without any stimulation. Different concentration of cGMP didn’t cause change in calcium con- tent in saponin-treated gland tissue. But at presence of tapsigargine in incubation medium cGMP (100mkM) inhib- ited Са2+-released channels.

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