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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(3): 3-8


Fluorine-containing analog of diasozide prevented of neonatal cardiomyocytesapoptosis during anoxia-reoxygenation

Nagibin V.S., V.E. DossenkO., Pivovar S.N., Moibenko A.A., Yagupolskiy L.N.

    A.A.Bogomoletz Institute of Physiology, National Academy ofSciences, Ukraine, Kiev


Abstract

In experiments on the primary culture of isolated neonatal rat cardiomyocytes it was established that anoxia-reoxygenation activated the process of programmed cell death, apoptosis. The amount of apoptotic cells (defined by fragmentation of a nucleus with Hoeсhst 33342 staining) during anoxia-reoxy- genation was increased in 2.1 fold (Р < 0.05). The amount of living and necrotic cells was not changed significantly. Apoptosis of neonatal cardiomyocytes during anoxia-reoxy- genation was prevented by activation of ATP-dependent po- tassium (кATP) channels with diazoxide. Synthesized by us the fluorine-containing analogue of diazoxide had the similar ef- fect: the amount of apoptotic cells was decreased to 3.7% that was similar to control meaning. Application of glybenclamide, which completely abrogated the action of diazoxide and its fluorine-containing analogue, allows us to assert that antiapoptotic effect of the substances mentioned above de- pends on КАТP channels opening.

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